FastQCFastQC Report
Thu 26 May 2016
SRR1546077_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546077_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences795169
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135041.6982553394309887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC1100.044.025
GTATTCG207.855016E-444.09
TAGCCGT207.855016E-444.044
TTATACG254.4415985E-544.01
CCGTAGA207.855016E-444.013
TATTACG302.526729E-644.01
TATCGCG254.4415985E-544.01
TGTCGCG207.855016E-444.01
CGTTTTT102750.043.1435551
CGACGGT1100.042.028
CGGTCTA1100.042.031
CGTAAGG1450.040.9655152
TCGACGG557.8216544E-1140.02
AGGGCGC1550.039.7419366
TCGACAG501.3460522E-939.61
GCGATAT950.039.368429
ACGTAAG452.3494067E-839.111111
GGCGATA3000.038.8666658
GTTTTTT117000.038.829062
ATGCGAG850.038.8235321
Produced by FastQC (version 0.11.5)