##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546077_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	795169
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.028343660278505	31.0	28.0	33.0	23.0	33.0
2	28.763609999886818	31.0	28.0	33.0	16.0	33.0
3	28.20122389077039	31.0	27.0	33.0	16.0	34.0
4	32.14254076806314	35.0	32.0	35.0	25.0	37.0
5	33.65778847012396	35.0	33.0	35.0	28.0	37.0
6	33.46115479853968	35.0	33.0	37.0	28.0	37.0
7	34.40339097726395	35.0	35.0	37.0	31.0	37.0
8	34.86336489475822	35.0	35.0	37.0	32.0	37.0
9	36.681018751988574	39.0	37.0	39.0	32.0	39.0
10	36.264175288523575	38.0	35.0	39.0	31.0	39.0
11	36.3036624918728	38.0	35.0	39.0	31.0	39.0
12	35.189161046268154	37.0	34.0	39.0	29.0	39.0
13	35.59800117962345	37.0	35.0	39.0	30.0	39.0
14	36.314758246360206	38.0	35.0	40.0	30.0	41.0
15	36.825403153291944	38.0	35.0	40.0	31.0	41.0
16	36.443865392136765	38.0	35.0	40.0	31.0	41.0
17	36.04648445802087	38.0	34.0	40.0	30.0	41.0
18	35.63570008388154	38.0	34.0	39.0	29.0	41.0
19	35.13611823398548	37.0	34.0	39.0	27.0	41.0
20	35.52111060667607	36.0	34.0	39.0	30.0	41.0
21	35.74867606760324	37.0	34.0	39.0	31.0	41.0
22	36.05970554687117	37.0	35.0	40.0	31.0	41.0
23	36.36802239523925	37.0	35.0	40.0	32.0	41.0
24	36.04856326139475	37.0	35.0	40.0	31.0	41.0
25	35.673104711074	36.0	34.0	40.0	30.0	41.0
26	35.80493706369338	37.0	34.0	40.0	31.0	41.0
27	36.087674444049	37.0	35.0	40.0	31.0	41.0
28	36.02834366027851	37.0	35.0	40.0	31.0	41.0
29	36.15761806609664	37.0	35.0	40.0	31.0	41.0
30	35.78525068255931	37.0	35.0	40.0	30.0	41.0
31	35.07908255980804	36.0	34.0	40.0	29.0	41.0
32	35.002897497261586	37.0	34.0	40.0	27.0	41.0
33	34.67937130345876	37.0	34.0	40.0	24.0	41.0
34	34.34671749024421	37.0	34.0	40.0	22.0	41.0
35	34.04963598933057	37.0	34.0	40.0	20.0	41.0
36	33.64196038829481	37.0	33.0	40.0	18.0	41.0
37	33.55645026403192	37.0	33.0	40.0	17.0	41.0
38	33.38892612765337	37.0	33.0	40.0	16.0	41.0
39	33.19111912058946	36.0	33.0	40.0	15.0	41.0
40	33.03145872135357	36.0	33.0	40.0	15.0	41.0
41	32.72308276605351	36.0	32.0	40.0	15.0	40.0
42	32.88210304979193	36.0	33.0	40.0	14.0	40.0
43	32.67117807661013	36.0	32.0	40.0	12.0	40.0
44	32.43129070675542	35.0	32.0	39.0	10.0	40.0
45	32.41644606366697	35.0	32.0	39.0	10.0	40.0
46	32.2498060160796	35.0	31.0	39.0	10.0	40.0
47	32.23000770905304	35.0	31.0	39.0	10.0	40.0
48	32.251683604365866	35.0	32.0	39.0	10.0	40.0
49	32.2287928729616	35.0	31.0	39.0	10.0	40.0
50	32.434355464058584	35.0	32.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	7.0
12	5.0
13	16.0
14	32.0
15	106.0
16	216.0
17	396.0
18	914.0
19	1570.0
20	2374.0
21	3590.0
22	5125.0
23	7314.0
24	10629.0
25	15920.0
26	22575.0
27	24950.0
28	23206.0
29	22153.0
30	23576.0
31	28858.0
32	37868.0
33	56403.0
34	87480.0
35	71277.0
36	79225.0
37	112804.0
38	128748.0
39	27829.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.303000997272274	22.642608049358063	27.4556729449966	22.598718008373062
2	37.598925511432164	20.48709142333265	29.500395513406584	12.413587551828604
3	27.416436002912587	18.743562689189343	41.14295200139845	12.697049306499625
4	24.719525031785697	24.694498905264165	37.157006875268024	13.428969187682116
5	20.023290646390894	29.54051277149889	37.612130251556586	12.824066330553629
6	18.509901668701872	36.62856575143146	34.804928260533295	10.056604319333374
7	77.2443342232909	4.310907492621065	16.289744695781653	2.1550135883063852
8	78.97478397673953	2.8690756304634615	16.0017556016394	2.1543847911576033
9	75.20552234807947	4.804890532704369	16.89175508602574	3.0978320331904285
10	40.099274493849734	26.847877620983716	22.81691061900049	10.23593726616606
11	33.460811475296445	21.038672282244402	32.50051246967626	13.000003772782891
12	30.824642308741918	20.97730168052326	34.06860679930933	14.129449211425497
13	20.02832102358115	30.485846404978062	34.612516333005935	14.873316238434848
14	16.33853935452715	32.31250212219038	35.92393566650611	15.425022856776357
15	15.062961458507562	22.58903453228182	46.894936799598575	15.453067209612046
16	17.631849330142398	19.90847228702326	45.25465655728531	17.205021825549036
17	17.873307435274764	19.49107673966163	36.38056815595176	26.255047669111846
18	19.97990364312492	22.086499850975073	39.51436738605253	18.41922911984748
19	24.21535547789212	22.651159690581498	34.721298239745266	18.41218659178112
20	25.51885196731764	22.65040513400296	33.811051487168136	18.019691411511264
21	20.877574452726403	24.927405369173094	36.428608258118715	17.76641191998179
22	20.546324114747936	21.316852140865652	35.31576306420396	22.821060680182452
23	18.231344531791354	25.57292852211291	34.999226579506995	21.19650036658874
24	17.794833551106745	22.826971373381006	42.381556625069635	16.99663845044261
25	17.53991918699044	23.510599633537023	40.052114707691075	18.89736647178147
26	17.091838338768238	28.78658499010902	35.151395489512296	18.97018118161045
27	16.969851691904488	28.24016026781728	36.439046290788504	18.35094174948973
28	15.660947546999443	25.44666605463744	41.530542563907794	17.361843834455318
29	16.4703352369119	23.365221732738576	40.526982314451395	19.63746071589813
30	18.579572392786943	26.103633315685094	37.629862331152246	17.686931960375716
31	24.724303890116442	24.115502490665506	33.69648464666002	17.463708972558035
32	25.08510769408767	24.34928927058273	33.28411947648864	17.28148355884095
33	24.348031676285167	24.828055419665503	32.41600213287993	18.4079107711694
34	19.77856279608486	24.95582700029805	33.46458425818914	21.801025945427956
35	19.56791575124282	25.47923774694436	34.43884256051229	20.51400394130053
36	25.72170192751478	25.226712811993423	30.51703474355766	18.534550516934136
37	20.16049418425517	29.521900375894933	31.337615022718442	18.979990417131454
38	20.967492445002257	29.388846899212623	29.614082037906407	20.029578617878716
39	20.688054992083444	28.206582500072315	29.949985474785862	21.155377033058382
40	23.37943254830105	25.39900323075975	29.958411356579546	21.263152864359654
41	19.59231308061557	24.29634455065527	30.853189699296628	25.258152669432537
42	21.229449337184924	25.457607125026254	28.50903392863655	24.80390960915227
43	21.018425014053616	25.504766911184916	29.572832944946292	23.903975129815173
44	19.633687933005437	28.008134119916644	30.877838547528892	21.480339399549024
45	18.37144053654003	31.598817358322574	28.17702903407955	21.85271307105785
46	20.910900701611858	29.28459233194453	29.407207775957062	20.39729919048655
47	20.89731868319816	26.0481734071625	30.461826353894583	22.592681555744754
48	21.01402343401214	25.353729836047435	32.39185632236669	21.240390407573738
49	20.227775479174866	24.91545822334623	33.0099639196196	21.846802377859298
50	19.519246851927075	29.018359619150143	30.822504398436056	20.639889130486726
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	253.0
1	644.0
2	1035.0
3	10977.5
4	20920.0
5	15188.0
6	9456.0
7	9544.5
8	9633.0
9	10102.5
10	10572.0
11	10582.0
12	10592.0
13	10212.0
14	9832.0
15	9176.5
16	8521.0
17	7984.0
18	7447.0
19	6956.5
20	6466.0
21	6115.0
22	5764.0
23	5681.0
24	5598.0
25	5784.5
26	5971.0
27	7100.0
28	8229.0
29	9079.0
30	9929.0
31	11818.0
32	13707.0
33	16403.5
34	19100.0
35	21236.5
36	23373.0
37	25804.5
38	28236.0
39	33056.5
40	37877.0
41	45872.5
42	53868.0
43	61170.0
44	68472.0
45	69588.0
46	70704.0
47	68649.5
48	66595.0
49	64290.5
50	61986.0
51	56557.5
52	51129.0
53	45903.0
54	40677.0
55	36666.5
56	32656.0
57	29686.0
58	26716.0
59	23997.0
60	21278.0
61	18325.5
62	15373.0
63	13225.5
64	11078.0
65	9579.0
66	8080.0
67	6551.5
68	5023.0
69	4250.0
70	3477.0
71	2898.0
72	2319.0
73	1870.0
74	1421.0
75	1143.0
76	865.0
77	653.5
78	442.0
79	334.5
80	227.0
81	172.5
82	118.0
83	98.0
84	78.0
85	57.0
86	36.0
87	27.0
88	18.0
89	13.0
90	8.0
91	6.5
92	5.0
93	3.5
94	2.0
95	4.5
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	795169.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.11077336161261
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.53450751973844	24.254714519473445
2	7.420297136064187	4.765429592239605
3	3.4991748481358846	3.370836315034398
4	2.1639641600918234	2.779462508294437
5	1.5233136794358728	2.4457390159504757
6	1.1096109555119735	2.1378279547204433
7	0.8745673920109982	1.965812472002524
8	0.7411890179187084	1.90401220580031
9	0.621443629242583	1.7959531982063939
>10	5.980317987430279	40.770675370747924
>50	0.4890530544228273	9.987713062485115
>100	0.041378380552105416	1.9252111471317896
>500	7.881596295639125E-4	0.18778844106372916
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	3.9407981478195624E-4	1.7088241968494597
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13504	1.6982553394309887	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024648848232262575	0.0
2	0.0	0.0	0.0	0.10350001068955153	0.0
3	0.0	0.0	0.0	0.14600669794722884	0.0
4	0.0	0.0	0.0	0.22033052093328587	0.0
5	1.2575942975644172E-4	0.0	0.0	0.4000407460552411	0.0
6	1.2575942975644172E-4	0.0	0.0	0.5667977499122828	0.0
7	1.2575942975644172E-4	0.0	0.0	0.6900419910735957	0.0
8	1.2575942975644172E-4	0.0	0.0	0.9664612176782545	0.0
9	1.2575942975644172E-4	0.0	0.0	1.0951131143190944	0.0
10	1.2575942975644172E-4	0.0	0.0	1.3149405975333546	0.0
11	3.772782892693251E-4	0.0	0.0	1.511502586242673	0.0
12	3.772782892693251E-4	0.0	0.0	1.6815293352733822	0.0
13	3.772782892693251E-4	0.0	0.0	1.748433351903809	0.0
14	3.772782892693251E-4	0.0	0.0	1.7753458698716877	0.0
15	3.772782892693251E-4	0.0	0.0	1.8168464816913135	0.0
16	3.772782892693251E-4	0.0	0.0	1.911794851157427	0.0
17	3.772782892693251E-4	0.0	0.0	2.033404219731906	0.0
18	3.772782892693251E-4	0.0	0.0	2.1981490727128445	0.0
19	3.772782892693251E-4	0.0	0.0	2.2821563717901476	0.0
20	3.772782892693251E-4	0.0	0.0	2.3669182274459897	0.0
21	3.772782892693251E-4	0.0	0.0	2.477838044491171	0.0
22	3.772782892693251E-4	0.0	0.0	2.6057353845534723	0.0
23	3.772782892693251E-4	0.0	0.0	2.7379085452274925	0.0
24	5.030377190257669E-4	0.0	0.0	2.844929819950224	0.0
25	5.030377190257669E-4	0.0	0.0	2.927553765300207	0.0
26	5.030377190257669E-4	0.0	0.0	3.015711125559472	0.0
27	5.030377190257669E-4	0.0	0.0	3.1068867121328925	0.0
28	6.287971487822085E-4	0.0	0.0	3.2008290061609546	0.0
29	6.287971487822085E-4	0.0	0.0	3.3068442054456346	0.0
30	6.287971487822085E-4	0.0	0.0	3.441532554714784	0.0
31	6.287971487822085E-4	0.0	0.0	3.5617585695619423	0.0
32	6.287971487822085E-4	0.0	0.0	3.671420792309559	0.0
33	6.287971487822085E-4	0.0	0.0	3.7890058591318323	0.0
34	7.545565785386502E-4	0.0	0.0	3.910740987136068	0.0
35	8.80316008295092E-4	0.0	0.0	4.059514392537938	0.0
36	8.80316008295092E-4	0.0	0.0	4.18565110058365	0.0
37	8.80316008295092E-4	0.0	0.0	4.313296921786438	0.0
38	8.80316008295092E-4	0.0	0.0	4.46307640262636	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	110	0.0	44.0	25
GTATTCG	20	7.855016E-4	44.0	9
TAGCCGT	20	7.855016E-4	44.0	44
TTATACG	25	4.4415985E-5	44.0	1
CCGTAGA	20	7.855016E-4	44.0	13
TATTACG	30	2.526729E-6	44.0	1
TATCGCG	25	4.4415985E-5	44.0	1
TGTCGCG	20	7.855016E-4	44.0	1
CGTTTTT	10275	0.0	43.143555	1
CGACGGT	110	0.0	42.0	28
CGGTCTA	110	0.0	42.0	31
CGTAAGG	145	0.0	40.965515	2
TCGACGG	55	7.8216544E-11	40.0	2
AGGGCGC	155	0.0	39.741936	6
TCGACAG	50	1.3460522E-9	39.6	1
GCGATAT	95	0.0	39.36842	9
ACGTAAG	45	2.3494067E-8	39.11111	1
GGCGATA	300	0.0	38.866665	8
GTTTTTT	11700	0.0	38.82906	2
ATGCGAG	85	0.0	38.823532	1
>>END_MODULE