FastQCFastQC Report
Thu 26 May 2016
SRR1546076_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546076_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences795374
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137431.727866387385054No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTCG207.855018E-444.027
ATCGTAG207.855018E-444.01
CCGTAGG207.855018E-444.02
CGGTATC207.855018E-444.024
ATACCGG207.855018E-444.02
GTAGACG351.4453326E-743.9999961
CGTTTTT100900.043.3458861
CGGTCTA1050.041.90476231
TTGTACG700.040.857141
GTAGGGT2350.040.2553184
ACGGTCT1100.040.00000430
GGCGATA2500.039.5999958
GCGATAT950.039.368429
GTTTTTT114100.039.218232
CGACCCG1600.038.532
TATGGGC2250.038.133334
GCGGGTA750.038.133335
AAGGGAT10200.037.7450945
ACCCGCT1700.037.5294134
ATAAGCG1000.037.39999833
Produced by FastQC (version 0.11.5)