##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546076_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	795374
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.017579151443222	31.0	28.0	33.0	23.0	33.0
2	28.731333938499372	31.0	28.0	33.0	16.0	33.0
3	28.211119548790883	31.0	27.0	33.0	16.0	34.0
4	32.13409037760852	35.0	32.0	35.0	25.0	37.0
5	33.6966936309208	35.0	33.0	35.0	28.0	37.0
6	33.568447045037935	35.0	33.0	37.0	28.0	37.0
7	34.46648872102935	35.0	35.0	37.0	32.0	37.0
8	34.90387918136625	35.0	35.0	37.0	32.0	37.0
9	36.802911586247475	39.0	37.0	39.0	32.0	39.0
10	36.26315167455813	38.0	35.0	39.0	31.0	39.0
11	36.36308327906117	38.0	35.0	39.0	32.0	39.0
12	35.14287869605997	37.0	34.0	39.0	29.0	39.0
13	35.59767857636785	37.0	35.0	39.0	30.0	39.0
14	36.328023043247576	38.0	35.0	40.0	30.0	41.0
15	36.85706598405279	38.0	35.0	40.0	32.0	41.0
16	36.4814326342073	38.0	35.0	40.0	31.0	41.0
17	36.10205513381126	38.0	34.0	40.0	30.0	41.0
18	35.72816310314393	38.0	34.0	39.0	29.0	41.0
19	35.213915717637235	37.0	34.0	39.0	28.0	41.0
20	35.50363351077606	36.0	34.0	39.0	30.0	41.0
21	35.65431734001866	36.0	34.0	39.0	30.0	40.0
22	36.048624923620835	37.0	35.0	40.0	31.0	41.0
23	36.4024622378906	37.0	35.0	40.0	32.0	41.0
24	35.97004302378504	37.0	35.0	40.0	31.0	41.0
25	35.60915493843148	36.0	34.0	40.0	30.0	41.0
26	35.78600381707222	37.0	34.0	40.0	31.0	41.0
27	36.119713493274865	37.0	35.0	40.0	31.0	41.0
28	36.05267710536175	37.0	35.0	40.0	31.0	41.0
29	36.201154425465255	37.0	35.0	40.0	31.0	41.0
30	35.84164808002273	37.0	35.0	40.0	31.0	41.0
31	35.128958200796106	36.0	34.0	40.0	29.0	41.0
32	35.091561957016445	37.0	34.0	40.0	28.0	41.0
33	34.7888238740517	37.0	34.0	40.0	25.0	41.0
34	34.37190554380707	37.0	34.0	40.0	22.0	41.0
35	34.10572007634144	37.0	34.0	40.0	20.0	41.0
36	33.68638024375954	37.0	33.0	40.0	18.0	41.0
37	33.59906785989987	37.0	33.0	40.0	18.0	41.0
38	33.474028570207224	37.0	33.0	40.0	17.0	41.0
39	33.234426823104606	36.0	33.0	40.0	15.0	41.0
40	33.049615652510646	36.0	33.0	40.0	15.0	41.0
41	32.74455916336214	36.0	32.0	40.0	15.0	40.0
42	32.920222436237545	36.0	33.0	40.0	15.0	40.0
43	32.72246515475739	36.0	32.0	40.0	14.0	40.0
44	32.472511045118395	35.0	32.0	39.0	10.0	40.0
45	32.44729146288413	35.0	32.0	39.0	11.0	40.0
46	32.26521862670894	35.0	31.0	39.0	10.0	40.0
47	32.24804431625876	35.0	31.0	39.0	10.0	40.0
48	32.26836808847159	35.0	32.0	39.0	10.0	40.0
49	32.30268653488799	35.0	32.0	39.0	11.0	40.0
50	32.53514070110413	35.0	32.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	6.0
13	11.0
14	17.0
15	69.0
16	174.0
17	431.0
18	832.0
19	1430.0
20	2170.0
21	3454.0
22	4818.0
23	7079.0
24	10475.0
25	16082.0
26	22413.0
27	24982.0
28	23178.0
29	21940.0
30	23070.0
31	28197.0
32	37521.0
33	56945.0
34	88184.0
35	71112.0
36	79560.0
37	116319.0
38	130028.0
39	24874.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.299735721811373	22.597545305730385	27.499138769937158	22.60358020252108
2	37.655367160606204	20.479296532197434	29.406040428779416	12.459295878416945
3	27.50806538810673	18.653111617930684	41.23720413289848	12.601618861064104
4	24.56869346999022	24.703724285681954	37.18803984037698	13.539542403950847
5	19.9884582598878	29.717340521565955	37.533034773578215	12.761166444968028
6	18.456474564167298	36.707888364467536	34.81154777500899	10.02408929635618
7	77.30841088594799	4.295086336742212	16.26555557511309	2.1309472021967024
8	79.06846338955008	2.8537518198985636	15.967582546072665	2.110202244478698
9	75.37586091574529	4.740662883121651	16.891550390130934	2.99192581100212
10	40.16248959608939	26.519222403548522	22.753069625107184	10.565218375254911
11	33.498077633918136	20.97742697146248	32.4715165444181	13.052978850201288
12	30.836436695189935	20.561144819921196	33.959872965422555	14.642545519466314
13	19.959289592066124	30.487418497461572	34.60875009743844	14.944541813033869
14	16.364754191110094	32.27500521767118	35.84590393953033	15.514336651688387
15	15.105095213069575	22.52713817650564	46.91792791818691	15.449838692237867
16	17.55212013467878	20.009454671638753	45.20829194819041	17.23013324549206
17	17.953315044243336	19.448586451153798	36.338125209021165	26.259973295581702
18	20.087153967818914	22.0677316583142	39.378958829431184	18.4661555444357
19	24.16196657170086	22.723900957285505	34.68657512063507	18.427557350378564
20	25.515543631046526	22.37098522204648	33.826979509010854	18.286491637896134
21	20.834098172683543	24.890303178127525	36.44939361859955	17.826205030589385
22	20.49840703870129	21.360265736622015	35.336332341766266	22.804994882910428
23	18.169062604510582	25.555650549301333	34.99888102955339	21.276405816634693
24	17.823564763243454	22.791542092147846	42.28539026923183	17.099502875376867
25	17.543319243525687	23.458901095585222	40.08328157571155	18.914498085177538
26	16.99150336822677	28.80443665495729	35.19187199983907	19.01218797697687
27	16.995275178720952	28.227098195314404	36.44713053230304	18.330496093661598
28	15.617307078179573	25.449034039332442	41.587479600791575	17.346179281696408
29	16.533354120200055	23.384219247800406	40.51980577690495	19.562620855094583
30	18.534928222446297	26.13173173877949	37.743753253186554	17.58958678558766
31	24.692283127182936	24.179442626990575	33.58897826682794	17.53929597899856
32	24.96875683640652	24.29888329263969	33.31376182776907	17.418598043184716
33	24.325285966098967	24.89005172409458	32.368168936877495	18.41649337292896
34	19.86700596197512	24.902750152758323	33.43621994181354	21.794023943453016
35	19.73914158622233	25.390948157722026	34.46240385026415	20.40750640579149
36	25.738206177219773	25.21530751570959	30.494082029334628	18.55240427773601
37	20.17201970393802	29.58507570023662	31.386618119274708	18.856286476550654
38	20.98019296582488	29.45218224382492	29.677987965409987	19.889636824940215
39	20.789967989901605	28.146632904771845	29.978852715829284	21.08454638949727
40	23.28175172937511	25.422882819906107	29.97684108356572	21.318524367153064
41	19.669111638047006	24.286059136959466	30.81481164835662	25.230017576636904
42	21.27628008961822	25.42388863603789	28.5044519936533	24.795379280690593
43	21.052988908362607	25.514286360881798	29.6280743398703	23.804650390885293
44	19.560232041781603	28.012482178195413	30.868748538423436	21.55853724159955
45	18.299441520592826	31.807929351474908	28.147261539854206	21.74536758807806
46	20.893064143409266	29.355623895173842	29.291000208706848	20.460311752710044
47	20.855848946533328	26.08911028019523	30.488172859560407	22.566867913711032
48	21.088318199991452	25.287097642115537	32.41695101926892	21.207633138624093
49	20.28429392964819	24.88967454304516	33.10467779937489	21.721353727931767
50	19.54049290019538	29.09008843638339	30.85391275047965	20.515505912941585
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	265.0
1	622.0
2	979.0
3	11211.0
4	21443.0
5	15462.5
6	9482.0
7	9475.0
8	9468.0
9	9904.0
10	10340.0
11	10357.0
12	10374.0
13	10007.0
14	9640.0
15	9070.5
16	8501.0
17	7942.5
18	7384.0
19	7033.5
20	6683.0
21	6170.5
22	5658.0
23	5570.5
24	5483.0
25	5745.0
26	6007.0
27	7034.5
28	8062.0
29	8911.5
30	9761.0
31	11880.5
32	14000.0
33	16622.5
34	19245.0
35	21242.0
36	23239.0
37	25719.0
38	28199.0
39	32903.0
40	37607.0
41	45601.5
42	53596.0
43	61333.0
44	69070.0
45	70052.0
46	71034.0
47	68616.0
48	66198.0
49	64193.5
50	62189.0
51	56678.5
52	51168.0
53	46028.5
54	40889.0
55	36897.5
56	32906.0
57	29768.5
58	26631.0
59	23750.5
60	20870.0
61	18212.0
62	15554.0
63	13418.0
64	11282.0
65	9611.5
66	7941.0
67	6551.5
68	5162.0
69	4248.0
70	3334.0
71	2850.5
72	2367.0
73	1940.5
74	1514.0
75	1192.0
76	870.0
77	672.5
78	475.0
79	360.5
80	246.0
81	182.0
82	118.0
83	101.0
84	84.0
85	53.5
86	23.0
87	20.0
88	17.0
89	10.0
90	3.0
91	5.5
92	8.0
93	5.0
94	2.0
95	1.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	795374.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.96197945353075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.38427673525021	24.09430704131343
2	7.508852235125831	4.799955617173804
3	3.4587243017965577	3.3164302520834723
4	2.1830482416655665	2.7909817218472512
5	1.5079032673039263	2.409778662373999
6	1.154832252185925	2.2146434829984707
7	0.9443557649940161	2.1128435690293443
8	0.7111191702529651	1.8183021046909684
9	0.5975136813236251	1.7187948005102303
>10	6.0138725591493385	40.86722625031804
>50	0.49342381510395683	10.014141962820286
>100	0.040887089739151526	1.9105127288394725
>500	7.939240726048839E-4	0.18841223285473607
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	3.9696203630244195E-4	1.7436695731465575
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13743	1.727866387385054	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02577403837691451	0.0
2	0.0	0.0	0.0	0.09391808130514702	0.0
3	0.0	0.0	0.0	0.13239054834580966	0.0
4	0.0	0.0	0.0	0.20996411750949867	0.0
5	0.0	0.0	0.0	0.38824502686786344	0.0
6	0.0	0.0	0.0	0.5636342148473549	0.0
7	0.0	0.0	0.0	0.6745254433763236	0.0
8	0.0	0.0	0.0	0.953513692929364	0.0
9	0.0	0.0	0.0	1.077606258187972	0.0
10	0.0	0.0	0.0	1.2912164591751805	0.0
11	1.257270164727537E-4	0.0	0.0	1.4825729782467116	0.0
12	1.257270164727537E-4	0.0	0.0	1.6538131746826021	0.0
13	1.257270164727537E-4	0.0	0.0	1.7159223208201424	0.0
14	2.514540329455074E-4	0.0	0.0	1.740690543065275	0.0
15	3.771810494182611E-4	0.0	0.0	1.7777800129247372	0.0
16	3.771810494182611E-4	0.0	0.0	1.8694350079333748	0.0
17	5.029080658910148E-4	0.0	0.0	1.987744130434236	0.0
18	5.029080658910148E-4	0.0	0.0	2.157098421623035	0.0
19	5.029080658910148E-4	0.0	0.0	2.2359292609514516	0.0
20	6.286350823637685E-4	0.0	0.0	2.327835709993035	0.0
21	6.286350823637685E-4	0.0	0.0	2.436841033274912	0.0
22	7.543620988365221E-4	0.0	0.0	2.5601792364346836	0.0
23	7.543620988365221E-4	0.0	0.0	2.69470714406053	0.0
24	7.543620988365221E-4	0.0	0.0	2.8001921108811705	0.0
25	7.543620988365221E-4	0.0	0.0	2.883926303852024	0.0
26	7.543620988365221E-4	0.0	0.0	2.9670318617405145	0.0
27	7.543620988365221E-4	0.0	0.0	3.0542864111726056	0.0
28	8.800891153092759E-4	0.0	0.0	3.149084581593062	0.0
29	8.800891153092759E-4	0.0	0.0	3.2529350971995563	0.0
30	0.0011315431482547833	0.0	0.0	3.382936832232384	0.0
31	0.0011315431482547833	0.0	0.0	3.5004915926344085	0.0
32	0.0011315431482547833	0.0	0.0	3.604342108240903	0.0
33	0.0011315431482547833	0.0	0.0	3.726297314219474	0.0
34	0.0011315431482547833	0.0	0.0	3.8472467040662632	0.0
35	0.0011315431482547833	0.0	0.0	3.9997535750477136	0.0
36	0.0011315431482547833	0.0	0.0	4.116302519317956	0.0
37	0.0013829971812002906	0.0	0.0	4.238760633362419	0.0
38	0.0013829971812002906	0.0	0.0	4.38674133175085	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTCTCG	20	7.855018E-4	44.0	27
ATCGTAG	20	7.855018E-4	44.0	1
CCGTAGG	20	7.855018E-4	44.0	2
CGGTATC	20	7.855018E-4	44.0	24
ATACCGG	20	7.855018E-4	44.0	2
GTAGACG	35	1.4453326E-7	43.999996	1
CGTTTTT	10090	0.0	43.345886	1
CGGTCTA	105	0.0	41.904762	31
TTGTACG	70	0.0	40.85714	1
GTAGGGT	235	0.0	40.255318	4
ACGGTCT	110	0.0	40.000004	30
GGCGATA	250	0.0	39.599995	8
GCGATAT	95	0.0	39.36842	9
GTTTTTT	11410	0.0	39.21823	2
CGACCCG	160	0.0	38.5	32
TATGGGC	225	0.0	38.13333	4
GCGGGTA	75	0.0	38.13333	5
AAGGGAT	1020	0.0	37.745094	5
ACCCGCT	170	0.0	37.52941	34
ATAAGCG	100	0.0	37.399998	33
>>END_MODULE