FastQCFastQC Report
Thu 26 May 2016
SRR1546075_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546075_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences781731
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135051.7275763657831147No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAG207.8549347E-444.01
CGACGTC302.5266745E-644.018
CGCATCG302.5266745E-644.021
CGTTCGA351.4452962E-744.014
ATCGTAG408.307325E-944.01
AGACGTC207.8549347E-444.026
CATAGCG207.8549347E-444.01
ACTACGT207.8549347E-444.026
CGTAATC207.8549347E-444.042
TCGACAG351.4452962E-744.01
AATACGG408.307325E-944.02
GTACGTA254.4415294E-544.011
TACCGGG600.044.03
CGTTTTT98900.043.488371
CGGTCTA1150.042.08695231
TACGGGA2250.042.044444
ACGGTCT1100.042.00000430
GTTAGCG603.6379788E-1240.3333321
GCACCGA1150.040.173919
ACACGCG501.3460522E-939.636
Produced by FastQC (version 0.11.5)