##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546075_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	781731
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.89418482828492	31.0	28.0	33.0	19.0	33.0
2	28.62804084781082	31.0	28.0	33.0	16.0	33.0
3	28.094664277097877	31.0	26.0	33.0	16.0	34.0
4	32.03302158926792	35.0	32.0	35.0	25.0	37.0
5	33.63508419136506	35.0	33.0	35.0	28.0	37.0
6	33.504851413081994	35.0	33.0	37.0	28.0	37.0
7	34.390003722508126	35.0	35.0	37.0	32.0	37.0
8	34.86685701347395	35.0	35.0	37.0	32.0	37.0
9	36.77284513470747	39.0	37.0	39.0	32.0	39.0
10	36.333412644503035	38.0	35.0	39.0	32.0	39.0
11	36.36439644839465	38.0	35.0	39.0	32.0	39.0
12	35.300013687572836	37.0	34.0	39.0	30.0	39.0
13	35.667394538530516	37.0	35.0	39.0	30.0	39.0
14	36.38439565528296	38.0	35.0	40.0	30.0	41.0
15	36.814468148250484	38.0	35.0	40.0	31.0	41.0
16	36.43215888841558	38.0	35.0	40.0	31.0	41.0
17	36.05150876708228	38.0	34.0	40.0	30.0	41.0
18	35.65170116062942	38.0	34.0	39.0	29.0	40.0
19	35.09596779454825	37.0	33.0	39.0	27.0	41.0
20	35.58344366540408	36.0	34.0	39.0	30.0	41.0
21	35.742053212678016	37.0	34.0	39.0	31.0	41.0
22	36.06549695483485	37.0	35.0	40.0	31.0	41.0
23	36.39043865472906	37.0	35.0	40.0	32.0	41.0
24	35.97074441208037	37.0	35.0	40.0	31.0	41.0
25	35.619454774084694	36.0	34.0	40.0	30.0	41.0
26	35.78287927688681	37.0	34.0	40.0	31.0	41.0
27	36.11384350882849	37.0	35.0	40.0	31.0	41.0
28	36.05617021712072	37.0	35.0	40.0	31.0	41.0
29	36.18840112519524	37.0	35.0	40.0	31.0	41.0
30	35.829848119110025	37.0	35.0	40.0	30.0	41.0
31	35.100450154848666	36.0	34.0	40.0	29.0	41.0
32	35.070154567236045	37.0	34.0	40.0	28.0	41.0
33	34.782907419559926	37.0	34.0	40.0	25.0	41.0
34	34.37770665356753	37.0	34.0	40.0	22.0	41.0
35	34.08096135371375	37.0	34.0	40.0	20.0	41.0
36	33.69395994274245	37.0	33.0	40.0	18.0	41.0
37	33.640403156584554	37.0	33.0	40.0	18.0	41.0
38	33.49580482288665	37.0	33.0	40.0	17.0	41.0
39	33.224866354282995	36.0	33.0	40.0	15.0	41.0
40	33.080688881469456	36.0	33.0	40.0	15.0	41.0
41	32.75893369969977	36.0	32.0	40.0	15.0	40.0
42	32.95782820433116	36.0	33.0	40.0	15.0	41.0
43	32.77531017703021	36.0	33.0	40.0	14.0	40.0
44	32.544188474040304	36.0	32.0	40.0	10.0	40.0
45	32.52846951189092	35.0	32.0	40.0	11.0	40.0
46	32.351600486612405	35.0	31.0	39.0	10.0	40.0
47	32.33410981526894	35.0	32.0	39.0	10.0	40.0
48	32.33056383845594	35.0	32.0	39.0	10.0	40.0
49	32.36755354463364	35.0	32.0	39.0	10.0	40.0
50	32.525737114173545	35.0	32.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	5.0
13	8.0
14	30.0
15	63.0
16	176.0
17	450.0
18	837.0
19	1420.0
20	2292.0
21	3393.0
22	4922.0
23	7072.0
24	10358.0
25	15912.0
26	22335.0
27	24400.0
28	22451.0
29	21675.0
30	23078.0
31	27642.0
32	36638.0
33	55493.0
34	86743.0
35	69023.0
36	77440.0
37	111379.0
38	128580.0
39	27915.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.30824286103532	22.519255344869272	27.469295704020947	22.703206090074463
2	37.69276132071007	20.33653520200683	29.510662875081067	12.460040602202037
3	27.582250160221356	18.765662357000043	41.078836581893256	12.573250900885343
4	24.602580683125012	24.71502345435962	37.06390049774156	13.618495364773816
5	19.935118346336527	29.582298770293107	37.61217093859652	12.87041194477384
6	18.43677684523193	36.61643199514923	34.90932302799813	10.037468131620724
7	77.13638067314716	4.354439058960179	16.315075134541164	2.1941051333514983
8	78.9074502610233	2.91724391126871	16.004994045266212	2.170311782441786
9	75.17304545937158	4.827363888601067	16.95723976662049	3.0423508854068726
10	40.09768065997127	26.828026520631777	22.782773102256403	10.291519717140552
11	33.477884336171904	21.01387817548492	32.439419698080286	13.06881779026289
12	30.862534554725347	20.933031950888477	34.00172181990992	14.202711674476259
13	20.01762754707182	30.517275124051622	34.52735020102823	14.937747127848327
14	16.357161222978238	32.37968047832311	35.80208537207812	15.46107292662054
15	15.066947581712892	22.53857145232823	46.91051013711878	15.4839708288401
16	17.54324697370323	19.955585744968538	45.23090423687944	17.270263044448793
17	17.859852046292136	19.553657204332435	36.310188543117775	26.27630220625765
18	20.119452855266072	22.052214892335087	39.419186395320125	18.409145857078713
19	24.204617700973866	22.672121228402098	34.655911048685546	18.467350021938493
20	25.479992478231	22.46488881725299	33.92010806786478	18.135010636651227
21	20.79794711991721	25.01781303287192	36.455634994646495	17.728604852564374
22	20.485947212020502	21.36195187347054	35.30293668793997	22.849164226568984
23	18.168398080669693	25.631067464383527	34.8650622784564	21.335472176490377
24	17.819940618959716	22.83893052725298	42.31442785305943	17.026701000727872
25	17.5840538497258	23.531751970946527	40.012101349441174	18.872092829886494
26	16.94457556371693	28.89894349846686	35.191773129119866	18.96470780869634
27	16.9835915423592	28.18821308097031	36.50296585398302	18.325229522687472
28	15.60524016573476	25.52821878625768	41.59435918493702	17.272181863070546
29	16.455916421377687	23.364175144646943	40.63891543254649	19.54099300142888
30	18.596294633320156	26.19468845421251	37.669223812283256	17.539793100184077
31	24.730374003333626	24.030516891360325	33.681918716284756	17.557190389021287
32	24.967923748706397	24.448051823453337	33.23764824472868	17.34637618311158
33	24.41824617419547	24.82976880794033	32.366888354178094	18.385096663686102
34	19.794021217017107	24.926733108959475	33.473535013962604	21.805710660060814
35	19.736072894640227	25.438162232276834	34.38906733902071	20.43669753406223
36	25.649232280669438	25.349256969469035	30.537614601442186	18.46389614841934
37	20.145420867280432	29.614662844379975	31.38534866853176	18.854567619807835
38	20.924461227711323	29.468448865402546	29.656748932817045	19.950340974069086
39	20.800761387229112	28.15482563695184	29.99177466417476	21.052638311644287
40	23.363919302164042	25.570816559660546	29.969260525679548	21.09600361249586
41	19.61928080119632	24.29902357716401	30.811238136903874	25.270457484735797
42	21.335472176490377	25.43125448523853	28.43881079297098	24.794462545300107
43	20.99481791050886	25.54817449992389	29.567843670009246	23.88916391955801
44	19.525258688730524	28.121182350450475	31.02588998005708	21.327668980761924
45	18.38599211237625	31.695685600289615	28.176956011722705	21.741366275611433
46	20.911413261083418	29.399243473778064	29.439282822351938	20.250060442786584
47	20.90348214411351	26.23869336127133	30.337673701055735	22.52015079355942
48	21.046881855779034	25.457734182218694	32.39183299626086	21.103550965741412
49	20.275516769835146	25.043397281161933	33.05113907469449	21.629946874308427
50	19.553273440608088	29.168345632960698	30.818145883942172	20.460235042489042
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	252.0
1	589.5
2	927.0
3	10978.5
4	21030.0
5	15169.5
6	9309.0
7	9448.5
8	9588.0
9	10017.0
10	10446.0
11	10378.5
12	10311.0
13	9904.5
14	9498.0
15	8909.5
16	8321.0
17	7914.5
18	7508.0
19	6878.5
20	6249.0
21	5845.0
22	5441.0
23	5441.0
24	5441.0
25	5603.5
26	5766.0
27	6829.5
28	7893.0
29	8752.0
30	9611.0
31	11574.5
32	13538.0
33	16374.5
34	19211.0
35	21216.5
36	23222.0
37	25447.0
38	27672.0
39	32361.5
40	37051.0
41	44979.0
42	52907.0
43	60490.0
44	68073.0
45	69052.5
46	70032.0
47	67549.0
48	65066.0
49	63045.5
50	61025.0
51	55529.5
52	50034.0
53	44855.0
54	39676.0
55	35904.0
56	32132.0
57	29182.5
58	26233.0
59	23271.0
60	20309.0
61	17863.0
62	15417.0
63	13184.5
64	10952.0
65	9457.5
66	7963.0
67	6387.5
68	4812.0
69	4062.5
70	3313.0
71	2805.0
72	2297.0
73	1844.5
74	1392.0
75	1145.0
76	898.0
77	667.5
78	437.0
79	325.5
80	214.0
81	163.0
82	112.0
83	93.5
84	75.0
85	59.0
86	43.0
87	29.5
88	16.0
89	11.0
90	6.0
91	4.0
92	2.0
93	4.0
94	6.0
95	4.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	781731.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.587205924151835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.86491738736687	23.647735620093723
2	7.535225901708769	4.760334644845551
3	3.5870580928314757	3.3991542792048954
4	2.2517123118328075	2.8450120190324353
5	1.5916260179023662	2.513750939085991
6	1.199251241734043	2.2728597556468806
7	0.8917504549306904	1.9717533676996257
8	0.7652086619077612	1.9336642862900104
9	0.6488815833346423	1.8446720573862934
>10	6.130323103899795	41.10487397848167
>50	0.49035805544413047	9.859206551714363
>100	0.04246231270178861	1.91233552977602
>500	8.16582936572858E-4	0.19293592882013258
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.08291468286429E-4	1.7417110419223867
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13505	1.7275763657831147	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.025840090772912935	0.0
2	0.0	0.0	0.0	0.09581300984609796	0.0
3	0.0	0.0	0.0	0.13393353979821704	0.0
4	0.0	0.0	0.0	0.20237140397400127	0.0
5	0.0	0.0	0.0	0.3713553639295359	0.0
6	0.0	0.0	0.0	0.5326640493980666	0.0
7	0.0	0.0	0.0	0.6389666010430698	0.0
8	0.0	0.0	0.0	0.9187303560943598	0.0
9	0.0	0.0	0.0	1.0373133469185691	0.0
10	1.2792124145006401E-4	0.0	0.0	1.2513255838645263	0.0
11	1.2792124145006401E-4	0.0	0.0	1.4412886274178713	0.0
12	1.2792124145006401E-4	0.0	0.0	1.6028531553693022	0.0
13	1.2792124145006401E-4	0.0	0.0	1.6693722009233356	0.0
14	1.2792124145006401E-4	0.0	0.0	1.6959798191449489	0.0
15	1.2792124145006401E-4	0.0	0.0	1.7319256879924168	0.0
16	1.2792124145006401E-4	0.0	0.0	1.8253081942509635	0.0
17	1.2792124145006401E-4	0.0	0.0	1.9395418628658707	0.0
18	1.2792124145006401E-4	0.0	0.0	2.108014137855605	0.0
19	1.2792124145006401E-4	0.0	0.0	2.1810571667235914	0.0
20	3.837637243501921E-4	0.0	0.0	2.263310524975983	0.0
21	3.837637243501921E-4	0.0	0.0	2.3742182413131885	0.0
22	5.116849658002561E-4	0.0	0.0	2.4848701151674937	0.0
23	5.116849658002561E-4	0.0	0.0	2.6088257981326057	0.0
24	5.116849658002561E-4	0.0	0.0	2.7066855478419045	0.0
25	5.116849658002561E-4	0.0	0.0	2.791625252164747	0.0
26	5.116849658002561E-4	0.0	0.0	2.8742623741414888	0.0
27	5.116849658002561E-4	0.0	0.0	2.9581787085327305	0.0
28	5.116849658002561E-4	0.0	0.0	3.0523287422399776	0.0
29	5.116849658002561E-4	0.0	0.0	3.154665735400029	0.0
30	6.396062072503201E-4	0.0	0.0	3.286680456576495	0.0
31	6.396062072503201E-4	0.0	0.0	3.3993790702940014	0.0
32	7.675274487003842E-4	0.0	0.0	3.5010764572468025	0.0
33	7.675274487003842E-4	0.0	0.0	3.6159497320689598	0.0
34	7.675274487003842E-4	0.0	0.0	3.726601605923265	0.0
35	8.954486901504482E-4	0.0	0.0	3.8797233319389917	0.0
36	0.0010233699316005121	0.0	0.0	3.9990738502119014	0.0
37	0.0010233699316005121	0.0	0.0	4.119831502140762	0.0
38	0.0010233699316005121	0.0	0.0	4.260289025252932	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAG	20	7.8549347E-4	44.0	1
CGACGTC	30	2.5266745E-6	44.0	18
CGCATCG	30	2.5266745E-6	44.0	21
CGTTCGA	35	1.4452962E-7	44.0	14
ATCGTAG	40	8.307325E-9	44.0	1
AGACGTC	20	7.8549347E-4	44.0	26
CATAGCG	20	7.8549347E-4	44.0	1
ACTACGT	20	7.8549347E-4	44.0	26
CGTAATC	20	7.8549347E-4	44.0	42
TCGACAG	35	1.4452962E-7	44.0	1
AATACGG	40	8.307325E-9	44.0	2
GTACGTA	25	4.4415294E-5	44.0	11
TACCGGG	60	0.0	44.0	3
CGTTTTT	9890	0.0	43.48837	1
CGGTCTA	115	0.0	42.086952	31
TACGGGA	225	0.0	42.04444	4
ACGGTCT	110	0.0	42.000004	30
GTTAGCG	60	3.6379788E-12	40.333332	1
GCACCGA	115	0.0	40.17391	9
ACACGCG	50	1.3460522E-9	39.6	36
>>END_MODULE