FastQCFastQC Report
Thu 26 May 2016
SRR1546074_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546074_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences686968
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68610.9987364768082356No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTC207.8542664E-444.0000047
AGGCGAT800.044.0000046
CGTCGAT207.8542664E-444.00000421
ACGTATG207.8542664E-444.0000041
CTGTCGG254.440963E-544.02
TCGATAG254.440963E-544.01
TTACGCG254.440963E-544.01
CGTTTTT90250.043.1224371
TAGGCGA750.041.0666665
AGGGTAT2250.040.088896
GCGGAAT2800.040.0714268
GTTAGCG501.3460522E-939.6000021
TCTACGG1000.039.6000022
CCCGATC501.3460522E-939.60000241
TAGTGCG452.3484972E-839.111111
GTTTTTT101900.039.0343482
ACGGTCT850.038.82353230
AGACGTG1600.038.50000424
TATACGG404.1213207E-738.5000042
GCTCACG800.038.50000444
Produced by FastQC (version 0.11.5)