##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546074_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	686968
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.873122183274912	28.0	25.0	30.0	16.0	33.0
2	26.00703380652374	30.0	25.0	31.0	10.0	33.0
3	26.308519465244377	30.0	25.0	31.0	16.0	33.0
4	27.979476482165108	32.0	19.0	35.0	10.0	35.0
5	31.17711742031652	33.0	30.0	35.0	19.0	37.0
6	29.75415739888903	33.0	28.0	35.0	17.0	36.0
7	29.79459887505677	32.0	28.0	35.0	17.0	35.0
8	32.462813406155746	33.0	32.0	35.0	28.0	35.0
9	27.065479032502243	31.0	23.0	34.0	10.0	35.0
10	31.589584958833598	34.0	30.0	37.0	17.0	37.0
11	33.70612604953943	35.0	32.0	37.0	27.0	39.0
12	33.19417935042098	35.0	31.0	39.0	24.0	39.0
13	33.562439589616986	35.0	32.0	39.0	25.0	39.0
14	33.25679216499167	36.0	31.0	39.0	22.0	40.0
15	32.05610304992372	34.0	27.0	38.0	18.0	40.0
16	30.5942445645212	34.0	27.0	38.0	17.0	40.0
17	30.885787984302034	33.0	27.0	38.0	17.0	40.0
18	31.749918482374724	34.0	29.0	38.0	18.0	39.0
19	31.434387045684808	34.0	27.0	37.0	18.0	39.0
20	32.14519453599003	34.0	30.0	37.0	22.0	39.0
21	32.98986124535641	35.0	31.0	37.0	24.0	39.0
22	33.345445493822126	35.0	31.0	38.0	26.0	39.0
23	34.567377228633646	35.0	33.0	38.0	29.0	40.0
24	34.184899442186534	35.0	33.0	38.0	27.0	40.0
25	32.90767109967277	35.0	31.0	38.0	23.0	40.0
26	26.56548048817412	33.0	21.0	36.0	0.0	39.0
27	29.918543512943835	34.0	24.0	37.0	15.0	39.0
28	31.409860430180153	34.0	29.0	37.0	20.0	39.0
29	32.728150365082506	35.0	30.0	37.0	23.0	39.0
30	32.47449080597641	35.0	31.0	38.0	22.0	39.0
31	31.314710437749646	34.0	29.0	37.0	19.0	39.0
32	31.647596394591886	34.0	30.0	37.0	19.0	39.0
33	31.426264396594892	34.0	30.0	37.0	18.0	39.0
34	31.39058587881823	34.0	30.0	38.0	16.0	39.0
35	30.588165678750684	34.0	29.0	37.0	12.0	39.0
36	30.330791245007045	34.0	27.0	37.0	10.0	39.0
37	30.407394522015583	34.0	28.0	38.0	10.0	39.0
38	30.101710705593273	34.0	27.0	37.0	10.0	39.0
39	29.973365571613233	34.0	27.0	37.0	10.0	39.0
40	29.854230473617402	34.0	27.0	37.0	10.0	39.0
41	29.253217034854607	33.0	26.0	36.0	10.0	38.0
42	29.45818000256198	33.0	26.0	37.0	10.0	38.0
43	29.00515453412677	33.0	25.0	36.0	9.0	38.0
44	28.815596941924515	33.0	24.0	36.0	9.0	38.0
45	28.300603521561413	32.0	24.0	35.0	9.0	38.0
46	27.836963293777877	31.0	23.0	35.0	8.0	38.0
47	27.935148362078	32.0	23.0	35.0	8.0	38.0
48	27.964520909270885	32.0	23.0	35.0	8.0	38.0
49	27.780337657649266	31.0	24.0	35.0	8.0	38.0
50	29.749737979061617	34.0	26.0	37.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	5.0
11	11.0
12	29.0
13	85.0
14	207.0
15	450.0
16	969.0
17	1703.0
18	2902.0
19	4549.0
20	6925.0
21	9784.0
22	13152.0
23	17534.0
24	22729.0
25	28031.0
26	32801.0
27	35870.0
28	39586.0
29	46361.0
30	56109.0
31	66589.0
32	74214.0
33	75615.0
34	67932.0
35	51962.0
36	25787.0
37	4947.0
38	127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.3375703089518	23.534138416927718	27.27492401392787	23.853367260192616
2	37.30028181807595	17.772880250608473	31.236389467922816	13.690448463392762
3	28.665381793620664	14.400088504850297	42.954984802785575	13.979544898743464
4	25.79698035425231	24.594304247068276	36.004588277765485	13.604127120913928
5	19.31298109955631	30.002125280944675	38.24428503219946	12.440608587299554
6	18.56403791734113	34.986782499330396	35.95393089634452	10.495248686983965
7	79.24008105181028	3.0609868290808304	16.205995039070235	1.4929370800386625
8	81.4572148921056	1.7415658371277847	15.561714665020787	1.239504605745828
9	69.084295047222	3.1323147511965623	15.342636047093897	12.440754154487545
10	40.87497525357804	27.03328248186233	22.695380279721906	9.396361984837721
11	34.18092254661062	20.61595299926634	32.87838734846456	12.324737105658489
12	31.452993443653853	20.86996774231114	34.37219783163117	13.304840982403837
13	19.477180887610483	31.235661631982858	34.91909375691444	14.368063723492213
14	15.699712359236528	32.55493705674791	36.41654341978084	15.328807164234723
15	13.68535361181307	21.07754655238672	46.92081727242026	18.316282563379954
16	15.833488605000523	18.137234922150668	45.083177091218225	20.946099381630585
17	17.081727242025828	18.872785923070655	36.97275564509555	27.072731189807968
18	19.430599387453274	21.73245333115953	40.496063863236714	18.34088341815048
19	24.538552014067612	22.1707561342013	35.45507214309837	17.835619708632716
20	25.876314471707563	22.150522295070513	34.41397561458467	17.55918761863726
21	20.57170057411699	24.791693353984463	37.126911297178324	17.50969477472022
22	20.174738852464742	21.011750183414655	35.68987201732832	23.123638946792283
23	17.55147255767372	25.767139080714095	35.596272315449916	21.085116046162266
24	16.959305236925154	22.442384506993047	43.95459468272176	16.64371557336004
25	16.935577785282575	22.468295466455498	41.58039384658383	19.0157329016781
26	13.45069930477111	40.75561015942518	29.338921172456363	16.454769363347346
27	16.197988843730712	29.127266481117026	36.9635849122521	17.711159762900163
28	15.043204341395814	25.390702332568623	42.868226758742765	16.6978665672928
29	15.763907489140689	23.144455054675035	41.58170395127575	19.509933504908524
30	18.10914045486835	26.075595952067637	38.27820218700143	17.53706140606258
31	25.251103399284975	23.864867068043928	34.010317802284824	16.873711730386276
32	25.2272303804544	23.679851172106996	33.79546063280968	17.29745781462892
33	24.380320480721082	24.48061627324708	32.91201336889055	18.227049877141294
34	19.343986910598456	24.39371266201628	33.65746293859394	22.604837488791325
35	19.28153858695019	24.90261555123383	34.81384867999674	21.00199718181924
36	25.789993129228726	24.73128297096808	30.450035518393868	19.028688381409324
37	19.561609856645433	30.0233780903914	31.022842403139595	19.392169649823572
38	20.244611102700564	30.060788857705163	29.585803123289583	20.108796916304687
39	20.405171711054955	28.347754189423668	29.892949889951204	21.35412420957017
40	23.2770667629351	24.984715445260914	29.89920927903483	21.839008512769155
41	18.96638562494905	23.71536956597687	30.930552805953116	26.38769200312096
42	20.66661038068731	24.92081144973274	27.736517567048246	26.676060602531702
43	20.689755563577926	24.850939199496917	29.490747749531277	24.96855748739388
44	18.919221856039876	27.584982124349317	31.10741693936253	22.38837908024828
45	17.69587520816108	32.46512210175729	27.691682873146927	22.147319816934704
46	20.283040840330262	29.190879342269216	28.87499854432812	21.6510812730724
47	20.30953406854468	25.55941470345052	30.049871318605813	24.08117990939898
48	20.399640157911286	24.868698396431856	32.94593052369252	21.78573092196434
49	19.870940131126925	24.843515272909364	32.56920264117106	22.716341954792654
50	19.03058075485321	29.617100068707714	30.766935286650902	20.58538388978817
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	158.0
1	359.5
2	561.0
3	6224.0
4	11887.0
5	9804.5
6	7722.0
7	7867.0
8	8012.0
9	8537.0
10	9062.0
11	9305.5
12	9549.0
13	9520.0
14	9491.0
15	9338.0
16	9185.0
17	8727.0
18	8269.0
19	7831.5
20	7394.0
21	7002.5
22	6611.0
23	6390.0
24	6169.0
25	6166.0
26	6163.0
27	6946.0
28	7729.0
29	8459.0
30	9189.0
31	11029.5
32	12870.0
33	15051.0
34	17232.0
35	18819.5
36	20407.0
37	22155.5
38	23904.0
39	27777.0
40	31650.0
41	38389.0
42	45128.0
43	51716.5
44	58305.0
45	59373.5
46	60442.0
47	58259.0
48	56076.0
49	54124.5
50	52173.0
51	47737.5
52	43302.0
53	39259.5
54	35217.0
55	31601.0
56	27985.0
57	25492.0
58	22999.0
59	20693.0
60	18387.0
61	16111.5
62	13836.0
63	11869.5
64	9903.0
65	8483.5
66	7064.0
67	5799.0
68	4534.0
69	3881.0
70	3228.0
71	2704.5
72	2181.0
73	1735.5
74	1290.0
75	1054.5
76	819.0
77	626.5
78	434.0
79	326.0
80	218.0
81	168.0
82	118.0
83	85.5
84	53.0
85	42.5
86	32.0
87	23.5
88	15.0
89	10.0
90	5.0
91	5.0
92	5.0
93	4.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	686968.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.996088026862516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.47050516680319	39.10265537578843
2	7.122995643025411	6.837518517952115
3	2.8391900743295913	4.088100501975521
4	1.6632147884438706	3.1931121357492613
5	1.1259714016385762	2.7021111254387433
6	0.7976578597490541	2.2970674111100613
7	0.6498385045442728	2.183279424714613
8	0.4997669886676468	1.91894883048099
9	0.4180910943088457	1.806006326912517
>10	3.285779156859747	30.428564322152496
>50	0.11309986559556458	3.413036031783831
>100	0.013228053365965626	0.8552033141575099
>500	3.3070133414913575E-4	0.08539335245299462
>1k	0.0	0.0
>5k	3.3070133414913575E-4	1.0890033293308479
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6861	0.9987364768082356	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016449092243015686	0.0
2	0.0	0.0	0.0	0.07263802680765334	0.0
3	0.0	0.0	0.0	0.10524507691770213	0.0
4	0.0	0.0	0.0	0.14804183018714118	0.0
5	0.0	0.0	0.0	0.2672613571520071	0.0
6	0.0	0.0	0.0	0.36522807467014473	0.0
7	0.0	0.0	0.0	0.4300054733262685	0.0
8	0.0	0.0	0.0	0.5931862910645037	0.0
9	0.0	0.0	0.0	0.6799443351073121	0.0
10	0.0	0.0	0.0	0.8150306855632286	0.0
11	0.0	0.0	0.0	0.9594333360505876	0.0
12	0.0	0.0	0.0	1.0687542942320458	0.0
13	0.0	0.0	0.0	1.1186838397130578	0.0
14	0.0	0.0	0.0	1.1355696335200476	0.0
15	0.0	0.0	0.0	1.1571135773427583	0.0
16	0.0	0.0	0.0	1.2034039431239882	0.0
17	0.0	0.0	0.0	1.2907442559187619	0.0
18	0.0	0.0	0.0	1.3927868547006557	0.0
19	0.0	0.0	0.0	1.4334001001502252	0.0
20	0.0	0.0	0.0	1.4891523331508891	0.0
21	0.0	0.0	0.0	1.5553854036869257	0.0
22	0.0	0.0	0.0	1.6185615632751453	0.0
23	0.0	0.0	0.0	1.6945476354065983	0.0
24	0.0	0.0	0.0	1.7545213168590095	0.0
25	0.0	0.0	0.0	1.805615399843952	0.0
26	0.0	0.0	0.0	1.8558360797009468	0.0
27	0.0	0.0	0.0	1.91828440334921	0.0
28	0.0	0.0	0.0	1.9850997426372117	0.0
29	0.0	0.0	0.0	2.0599212772647344	0.0
30	0.0	0.0	0.0	2.161963876046628	0.0
31	0.0	0.0	0.0	2.2421713966298285	0.0
32	0.0	0.0	0.0	2.3185941703252553	0.0
33	0.0	0.0	0.0	2.4012763331043074	0.0
34	0.0	0.0	0.0	2.491382422470916	0.0
35	0.0	0.0	0.0	2.599247708772461	0.0
36	0.0	0.0	0.0	2.686442454379243	0.0
37	0.0	0.0	0.0	2.7877572172211806	0.0
38	0.0	0.0	0.0	2.8969326082146476	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTC	20	7.8542664E-4	44.000004	7
AGGCGAT	80	0.0	44.000004	6
CGTCGAT	20	7.8542664E-4	44.000004	21
ACGTATG	20	7.8542664E-4	44.000004	1
CTGTCGG	25	4.440963E-5	44.0	2
TCGATAG	25	4.440963E-5	44.0	1
TTACGCG	25	4.440963E-5	44.0	1
CGTTTTT	9025	0.0	43.122437	1
TAGGCGA	75	0.0	41.066666	5
AGGGTAT	225	0.0	40.08889	6
GCGGAAT	280	0.0	40.071426	8
GTTAGCG	50	1.3460522E-9	39.600002	1
TCTACGG	100	0.0	39.600002	2
CCCGATC	50	1.3460522E-9	39.600002	41
TAGTGCG	45	2.3484972E-8	39.11111	1
GTTTTTT	10190	0.0	39.034348	2
ACGGTCT	85	0.0	38.823532	30
AGACGTG	160	0.0	38.500004	24
TATACGG	40	4.1213207E-7	38.500004	2
GCTCACG	80	0.0	38.500004	44
>>END_MODULE