FastQCFastQC Report
Thu 26 May 2016
SRR1546073_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546073_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1899499
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410582.1615173264108063No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAA207.857781E-444.016
CGACGAA207.857781E-444.017
TCGATAG750.044.01
CAATACG207.857781E-444.010
CGTTTTT283250.043.3631061
TACGTAG700.040.8571431
ACGATTG1000.039.6000021
GTTTTTT322800.038.9907042
GCGTAAG850.038.8235321
CGACGGT1200.038.50000428
TCGATTG800.038.51
TCTAGCG404.126432E-738.51
TAGTGCG800.038.51
GGGCGAT22800.037.5350887
TTACGGG4400.037.53
ATAGCGG2300.037.3043482
AGGGATC17950.037.2590526
CGTAAGG1950.037.2307662
TCGTCCC6950.037.03597338
TACGGGA5650.036.991154
Produced by FastQC (version 0.11.5)