##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546073_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1899499
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.043728372586667	31.0	28.0	33.0	23.0	33.0
2	28.89111707876656	31.0	28.0	33.0	16.0	33.0
3	28.259411560627303	31.0	27.0	33.0	16.0	34.0
4	32.077832628498356	35.0	32.0	35.0	25.0	37.0
5	33.662920591166404	35.0	33.0	35.0	28.0	37.0
6	33.50655251726903	35.0	33.0	37.0	28.0	37.0
7	34.30514677817677	35.0	35.0	37.0	31.0	37.0
8	34.80397304762993	35.0	35.0	37.0	32.0	37.0
9	36.63482318232334	39.0	37.0	39.0	32.0	39.0
10	36.29418336098097	38.0	35.0	39.0	31.0	39.0
11	36.366420303458966	39.0	35.0	39.0	32.0	39.0
12	35.36881356610348	37.0	34.0	39.0	29.0	39.0
13	35.787539240610286	37.0	35.0	39.0	30.0	39.0
14	36.56227826390011	38.0	35.0	40.0	31.0	41.0
15	37.02085760508429	39.0	35.0	40.0	32.0	41.0
16	36.60948913371368	38.0	35.0	40.0	31.0	41.0
17	36.24018438546164	38.0	34.0	40.0	30.0	41.0
18	35.818996482756766	38.0	34.0	39.0	29.0	41.0
19	35.25442603549673	37.0	34.0	39.0	28.0	41.0
20	35.57996661224881	36.0	34.0	39.0	30.0	41.0
21	35.80756662677896	37.0	34.0	39.0	31.0	41.0
22	36.11221011435121	37.0	35.0	40.0	31.0	41.0
23	36.3983755716639	37.0	35.0	40.0	32.0	41.0
24	36.058910270550285	37.0	35.0	40.0	31.0	41.0
25	35.69540863143387	36.0	35.0	40.0	30.0	41.0
26	35.78537024762846	37.0	34.0	40.0	31.0	41.0
27	36.08608585737608	37.0	35.0	40.0	31.0	41.0
28	36.00086285910127	37.0	35.0	40.0	31.0	41.0
29	36.10043806287869	37.0	35.0	40.0	31.0	41.0
30	35.69952708582632	37.0	35.0	40.0	30.0	41.0
31	34.974842313683766	36.0	34.0	40.0	28.0	41.0
32	34.855485578039264	37.0	34.0	40.0	26.0	41.0
33	34.45829294987784	37.0	34.0	40.0	23.0	41.0
34	34.03516980003675	37.0	34.0	40.0	20.0	41.0
35	33.68794139928476	37.0	33.0	40.0	17.0	41.0
36	33.255621613909774	37.0	33.0	40.0	15.0	41.0
37	33.164120644443614	37.0	33.0	40.0	15.0	41.0
38	32.98219004063703	36.0	33.0	40.0	12.0	41.0
39	32.76990774935917	36.0	32.0	40.0	10.0	41.0
40	32.60295899076546	36.0	32.0	40.0	10.0	41.0
41	32.30002016321146	36.0	31.0	40.0	10.0	40.0
42	32.474362450309265	36.0	32.0	40.0	10.0	40.0
43	32.258110691292806	35.0	31.0	40.0	10.0	40.0
44	32.1208323879086	35.0	31.0	39.0	10.0	40.0
45	32.07986211100927	35.0	31.0	39.0	10.0	40.0
46	31.89304390262906	35.0	31.0	39.0	10.0	40.0
47	31.842312104402264	35.0	31.0	39.0	10.0	40.0
48	31.832427392696705	35.0	31.0	39.0	10.0	40.0
49	31.79686222524992	35.0	31.0	39.0	10.0	40.0
50	31.986734923261345	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	8.0
11	3.0
12	15.0
13	28.0
14	75.0
15	206.0
16	525.0
17	1207.0
18	2288.0
19	4063.0
20	6351.0
21	9297.0
22	13366.0
23	19064.0
24	28192.0
25	42967.0
26	59708.0
27	64593.0
28	58669.0
29	54725.0
30	56801.0
31	66923.0
32	86863.0
33	126889.0
34	194712.0
35	166701.0
36	187979.0
37	269467.0
38	308667.0
39	69146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.1414725672401	22.28434971537232	25.58727327574271	24.986904441644874
2	40.79233524208226	19.30282669272266	28.230549213239915	11.674288851955174
3	27.522467766500537	18.432123417806483	41.43740007233486	12.608008743358118
4	23.41733267561604	24.314095453590657	38.66756444725688	13.601007423536416
5	19.902037326684564	28.532470930492725	38.93474016043177	12.630751582390937
6	18.394587204310188	35.79070060052677	36.21039021341944	9.604321981743608
7	74.93786519498036	4.157306742462091	18.818383163139334	2.086444899418215
8	76.26116149574177	2.9536735739265985	18.566737860878053	2.218427069453577
9	72.31243606866863	5.040171118805538	19.438915208694503	3.2084776038313256
10	37.15274396038113	28.52473204776628	24.644340428713043	9.678183563139543
11	30.867086531764425	21.33062454889421	35.03334300254962	12.768945916791743
12	28.60928065768921	20.52378021783639	37.173960081052954	13.692979043421449
13	19.967528279825363	28.606227220967213	37.08688448901526	14.339360010192163
14	16.284925656712637	30.30925522993168	38.440557220614494	14.965261892741191
15	15.286241266776134	22.186323867504008	47.24766899061279	15.279765875107069
16	17.74989089228265	19.587006889711446	45.5157912691715	17.147310948834406
17	18.0689223842708	19.429912834910677	38.07593475963925	24.42523002117927
18	20.3424166056418	21.534257190975094	40.572171925334	17.551154278049104
19	23.496300866702217	22.817806168889796	36.03681812941202	17.649074834995965
20	24.175743182807675	22.349788023052394	36.004546461988134	17.469922332151793
21	20.63707324931469	24.352737221762162	37.73068582821049	17.279503700712663
22	20.63954758596872	21.203117243020397	36.771064370131285	21.3862708008796
23	18.03207056176392	25.176901909398214	36.71036415391637	20.080663374921492
24	17.629438078145867	22.36252822454763	43.23397906500609	16.774054632300412
25	18.086874486377724	23.130204332826708	40.34932369008881	18.43359749070676
26	16.92219895877808	27.268242836663774	36.547321162053784	19.262237042504367
27	17.15536570432519	26.638339899099712	37.92452641459669	18.281767981978405
28	15.910247912739095	24.74736759535014	42.054141644717895	17.288242847192866
29	16.651390708813217	22.67976977087116	41.183543660723174	19.48529585959245
30	18.40816973317701	25.239392071277745	38.72078900804897	17.631649187496283
31	23.514621487034212	23.51509529618073	35.25519097404105	17.715092242744007
32	23.728730575799197	23.842707998266913	34.99070017936308	17.437861246570808
33	23.079927917835178	24.12767787716656	33.711889292913554	19.080504912084713
34	18.931307676392564	25.464819934098415	34.45603288024895	21.147839509260073
35	19.86534344055985	25.20675188562879	34.684250952488	20.24365372132336
36	24.41359537435924	25.673032731262296	31.047871043891046	18.865500850487418
37	19.98763884582198	29.0958300057015	31.682933236606075	19.233597911870444
38	20.60001084496491	29.064295374727756	30.323838022552263	20.01185575775507
39	20.49366701430219	28.033339317367368	30.457820720095143	21.015172948235296
40	22.771741390756194	25.600539931845184	30.60091108234329	21.026807595055327
41	19.02880706965363	24.65571184822945	31.74337022551736	24.572110856599554
42	20.91135609968734	25.304725088036374	29.868875950974445	23.91504286130185
43	20.572477269006196	25.820176793986207	30.349160489160564	23.258185447847037
44	20.070871319226807	27.201856910690665	31.518416171843207	21.208855598239325
45	19.11704086182725	30.457504847330796	28.813071236152272	21.612383054689683
46	20.78348027558846	28.548211923249234	30.113624697880866	20.554683103281445
47	20.53109793687704	26.098302762991715	31.280669271213096	22.08993002891815
48	20.672977453528535	25.442761486055005	32.47256250200711	21.41169855840935
49	20.450234509204794	25.196012211641072	32.82497121609435	21.528782063059786
50	19.430597225900094	27.945684625261713	31.660927434023396	20.9627907148148
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	906.0
1	1711.0
2	2516.0
3	33160.5
4	63805.0
5	45334.5
6	26864.0
7	26507.0
8	26150.0
9	27030.0
10	27910.0
11	27960.0
12	28010.0
13	26945.5
14	25881.0
15	24733.0
16	23585.0
17	22275.0
18	20965.0
19	19277.0
20	17589.0
21	16841.0
22	16093.0
23	15334.5
24	14576.0
25	16020.0
26	17464.0
27	19123.0
28	20782.0
29	23628.5
30	26475.0
31	31422.5
32	36370.0
33	42486.0
34	48602.0
35	54376.5
36	60151.0
37	64231.5
38	68312.0
39	77693.5
40	87075.0
41	105488.5
42	123902.0
43	133870.0
44	143838.0
45	146332.5
46	148827.0
47	146946.0
48	145065.0
49	141099.0
50	137133.0
51	124772.5
52	112412.0
53	103641.5
54	94871.0
55	86546.0
56	78221.0
57	71628.5
58	65036.0
59	58691.0
60	52346.0
61	47327.5
62	42309.0
63	36340.5
64	30372.0
65	26060.0
66	21748.0
67	18120.5
68	14493.0
69	12514.0
70	10535.0
71	9107.5
72	7680.0
73	6269.5
74	4859.0
75	3915.0
76	2971.0
77	2159.5
78	1348.0
79	966.5
80	585.0
81	487.0
82	389.0
83	295.5
84	202.0
85	157.5
86	113.0
87	92.0
88	71.0
89	64.0
90	57.0
91	38.5
92	20.0
93	13.0
94	6.0
95	6.5
96	7.0
97	4.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1899499.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.657040670590614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.75471716660827	21.228083343365647
2	6.968306540739899	3.8544547480477207
3	3.091108776257454	2.5647276342651604
4	1.8890199035077204	2.0897880119547274
5	1.3317244112105533	1.841577810143431
6	1.045915009965493	1.7356148381158096
7	0.840983930062945	1.6281388729944806
8	0.6882396724554058	1.5227738089770502
9	0.607697210976665	1.5126395831447499
>10	5.648116168178521	33.93558145627232
>50	0.9338170026189146	17.777370867217225
>100	0.19745053294193773	7.5925688340593895
>500	0.002516517879393213	0.45025482155232105
>1k	1.9357829841486253E-4	0.06826098662059565
>5k	0.0	0.0
>10k+	1.9357829841486253E-4	2.198164383269346
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41058	2.1615173264108063	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.023269293640059828	0.0
2	0.0	0.0	0.0	0.08639120104827641	0.0
3	0.0	0.0	0.0	0.13466708853229192	0.0
4	0.0	0.0	0.0	0.20157946911264496	0.0
5	0.0	0.0	0.0	0.3716769527122678	0.0
6	0.0	0.0	0.0	0.5683077485168457	0.0
7	0.0	0.0	0.0	0.7079235103572047	0.0
8	0.0	0.0	0.0	1.0228486564088741	0.0
9	5.264546072411725E-5	0.0	0.0	1.1845755117533623	0.0
10	1.052909214482345E-4	0.0	0.0	1.4343782228892987	0.0
11	1.052909214482345E-4	0.0	0.0	1.6216381266849837	0.0
12	1.052909214482345E-4	0.0	0.0	1.7884715917197114	0.0
13	2.10581842896469E-4	0.0	0.0	1.8554892632215125	0.0
14	2.10581842896469E-4	0.0	0.0	1.885286593991363	0.0
15	2.10581842896469E-4	0.0	0.0	1.9224016438018656	0.0
16	2.10581842896469E-4	0.0	0.0	1.9987375618518357	0.0
17	2.632273036205863E-4	0.0	0.0	2.1011329829602436	0.0
18	2.632273036205863E-4	0.0	0.0	2.2421175267794298	0.0
19	2.632273036205863E-4	0.0	0.0	2.3105566257207824	0.0
20	2.632273036205863E-4	0.0	0.0	2.3861028618598903	0.0
21	2.632273036205863E-4	0.0	0.0	2.4790747454986817	0.0
22	2.632273036205863E-4	0.0	0.0	2.5841024396432952	0.0
23	2.632273036205863E-4	0.0	0.0	2.698027216650285	0.0
24	2.632273036205863E-4	0.0	0.0	2.79436840977542	0.0
25	2.632273036205863E-4	0.0	0.0	2.8674929547212185	0.0
26	3.158727643447035E-4	0.0	0.0	2.941723054342224	0.0
27	4.21163685792938E-4	0.0	0.0	3.021744154642882	0.0
28	5.264546072411726E-4	0.0	0.0	3.1063454100265386	0.0
29	5.264546072411726E-4	0.0	0.0	3.1983696753722954	0.0
30	5.791000679652898E-4	0.0	0.0	3.3262981449319007	0.0
31	6.31745528689407E-4	0.0	0.0	3.431589066380135	0.0
32	6.31745528689407E-4	0.0	0.0	3.534826814860129	0.0
33	6.31745528689407E-4	0.0	0.0	3.6382751451830195	0.0
34	7.370364501376415E-4	0.0	0.0	3.747251248881942	0.0
35	7.370364501376415E-4	0.0	0.0	3.8798651644459934	0.0
36	7.896819108617588E-4	0.0	0.0	3.9921579321705356	0.0
37	8.42327371585876E-4	0.0	0.0	4.1100837641925585	0.0
38	8.42327371585876E-4	0.0	0.0	4.243118843442402	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGAA	20	7.857781E-4	44.0	16
CGACGAA	20	7.857781E-4	44.0	17
TCGATAG	75	0.0	44.0	1
CAATACG	20	7.857781E-4	44.0	10
CGTTTTT	28325	0.0	43.363106	1
TACGTAG	70	0.0	40.857143	1
ACGATTG	100	0.0	39.600002	1
GTTTTTT	32280	0.0	38.990704	2
GCGTAAG	85	0.0	38.823532	1
CGACGGT	120	0.0	38.500004	28
TCGATTG	80	0.0	38.5	1
TCTAGCG	40	4.126432E-7	38.5	1
TAGTGCG	80	0.0	38.5	1
GGGCGAT	2280	0.0	37.535088	7
TTACGGG	440	0.0	37.5	3
ATAGCGG	230	0.0	37.304348	2
AGGGATC	1795	0.0	37.259052	6
CGTAAGG	195	0.0	37.230766	2
TCGTCCC	695	0.0	37.035973	38
TACGGGA	565	0.0	36.99115	4
>>END_MODULE