FastQCFastQC Report
Thu 26 May 2016
SRR1546072_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546072_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1899908
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT418752.204054091040198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG408.3182385E-944.0000049
CGTTTTT289450.043.3691521
TCGCCGT603.6379788E-1240.33333225
GTTTTTT328000.039.1237832
ACGTTAG404.126432E-738.5000041
TACGTAG700.037.7142831
CGGGATA4100.037.5609746
CGACCCG5100.037.5294132
CGTTGAT2950.037.28813625
TAGACGG1950.037.2307662
ACCCGCT5100.037.0980434
GCGTAAG950.037.052631
TACGGGA5050.037.0297054
ATAGGGA19000.036.936844
TAGGGCG4250.036.752945
AAGGGAT23000.036.7304345
GGGCGAT24800.036.6370967
TTACGGG4150.036.5783123
TCAGACG5450.036.33027622
AGGGATC15300.036.2352946
Produced by FastQC (version 0.11.5)