##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546072_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1899908
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.014389117788863	31.0	28.0	33.0	23.0	33.0
2	28.85748941527695	31.0	28.0	33.0	16.0	33.0
3	28.259951534495354	31.0	27.0	33.0	16.0	34.0
4	32.0537141798445	35.0	32.0	35.0	25.0	37.0
5	33.697160599355335	35.0	33.0	35.0	28.0	37.0
6	33.603203944612055	35.0	33.0	37.0	28.0	37.0
7	34.362467024719095	35.0	35.0	37.0	31.0	37.0
8	34.842752385905	35.0	35.0	37.0	32.0	37.0
9	36.753910189335485	39.0	37.0	39.0	32.0	39.0
10	36.28996351402279	38.0	35.0	39.0	31.0	39.0
11	36.41469113241273	39.0	35.0	39.0	32.0	39.0
12	35.316756916650704	37.0	34.0	39.0	29.0	39.0
13	35.78454325156797	37.0	35.0	39.0	30.0	39.0
14	36.57063078843818	38.0	35.0	40.0	31.0	41.0
15	37.04966345738846	39.0	35.0	40.0	32.0	41.0
16	36.63460704413056	38.0	35.0	40.0	31.0	41.0
17	36.279969872225394	38.0	34.0	40.0	31.0	41.0
18	35.887962469761696	38.0	34.0	39.0	29.0	41.0
19	35.31557370146344	37.0	34.0	39.0	29.0	41.0
20	35.56012659560358	36.0	34.0	39.0	30.0	41.0
21	35.686274282754745	36.0	34.0	39.0	30.0	40.0
22	36.077702183474145	37.0	35.0	40.0	31.0	41.0
23	36.413404754335474	37.0	35.0	40.0	32.0	41.0
24	35.96301452491384	37.0	35.0	40.0	31.0	41.0
25	35.613839722765526	36.0	34.0	40.0	30.0	41.0
26	35.749744724481396	37.0	34.0	40.0	30.0	41.0
27	36.103569225457235	37.0	35.0	40.0	31.0	41.0
28	36.01006575055213	37.0	35.0	40.0	31.0	41.0
29	36.10876263482232	37.0	35.0	40.0	31.0	41.0
30	35.73956159982483	37.0	35.0	40.0	30.0	41.0
31	35.00160849893784	36.0	34.0	40.0	28.0	41.0
32	34.920901433122026	37.0	34.0	40.0	26.0	41.0
33	34.53206944757325	37.0	34.0	40.0	23.0	41.0
34	34.039968251094265	37.0	34.0	40.0	20.0	41.0
35	33.72814841560749	37.0	33.0	40.0	17.0	41.0
36	33.2774697511669	37.0	33.0	40.0	15.0	41.0
37	33.18914968514265	37.0	33.0	40.0	15.0	41.0
38	33.03764866509326	37.0	33.0	40.0	12.0	41.0
39	32.7794182665687	36.0	32.0	40.0	10.0	41.0
40	32.59885215494645	36.0	32.0	40.0	10.0	41.0
41	32.28835501508494	36.0	31.0	40.0	10.0	40.0
42	32.46072651938936	36.0	32.0	40.0	10.0	40.0
43	32.264925985889846	35.0	31.0	40.0	10.0	40.0
44	32.119457889539916	35.0	31.0	39.0	10.0	40.0
45	32.06626952462961	35.0	31.0	39.0	10.0	40.0
46	31.855518267200306	35.0	31.0	39.0	10.0	40.0
47	31.80910075645768	35.0	31.0	39.0	10.0	40.0
48	31.80432052499384	35.0	31.0	39.0	10.0	40.0
49	31.828819606001975	35.0	31.0	39.0	10.0	40.0
50	32.04358579468058	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	7.0
12	12.0
13	37.0
14	82.0
15	209.0
16	500.0
17	1168.0
18	2232.0
19	3836.0
20	6215.0
21	8919.0
22	13173.0
23	18637.0
24	27743.0
25	42658.0
26	59772.0
27	64709.0
28	59208.0
29	54007.0
30	56733.0
31	66427.0
32	86219.0
33	127942.0
34	195243.0
35	167291.0
36	190559.0
37	275111.0
38	309453.0
39	61803.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.259162022582146	22.121123759676784	25.536447027961355	25.08326718977972
2	40.99351126475598	19.291723599247966	28.06625373439135	11.64851140160471
3	27.438539129263102	18.35857315196315	41.509746787739196	12.693140931034556
4	23.3144973335551	24.358758424092112	38.69808432829379	13.628659914058996
5	19.824328335898368	28.528802447276398	39.00125690296583	12.645612313859408
6	18.37131061082958	35.76141581592372	36.37855096141497	9.488722611831731
7	74.7804630540005	4.176781191510326	18.941338212166063	2.101417542323102
8	76.13368647323976	2.9730386945052074	18.685167913393702	2.208106918861334
9	72.22218128456747	5.074929943976235	19.5606839910143	3.1422047804420004
10	37.187274331178145	28.101992306995914	24.737039898774047	9.973693463051896
11	30.875442389842036	21.293820542889446	35.017327154788546	12.813409912479973
12	28.605753541750445	20.179608696842163	37.183116235101906	14.031521526305484
13	19.987388863039683	28.568962286594928	37.13111371708525	14.312535133280138
14	16.31400046739105	30.246727736290392	38.49007425622714	14.949197540091417
15	15.282371567465372	22.184547883371195	47.30423788941359	15.22884265974984
16	17.824231489103685	19.44520471517568	45.55178461272862	17.178779182992017
17	18.069822328239052	19.38467546849637	38.17169041869396	24.37381178457062
18	20.323352499173645	21.446933219924333	40.656179141305785	17.573535139596235
19	23.510191019775696	22.777260793680536	36.03379742598062	17.678750760563144
20	24.229225836198385	22.119755272360557	36.090221210711256	17.560797680729802
21	20.589418013924885	24.300124006004502	37.795040601965994	17.315417378104627
22	20.695896853952927	21.181604582958755	36.80015032306827	21.32234824002004
23	18.065453695652632	25.102215475696717	36.78883398564562	20.04349684300503
24	17.64369643161669	22.348818995446095	43.28614859245816	16.721335980479054
25	18.10182387778777	23.06606425153218	40.40206157350777	18.430050297172286
26	16.933293612111743	27.21458091654964	36.64266901344696	19.209456457891648
27	17.190569227562598	26.476334643572212	38.0768963549814	18.25619977388379
28	15.90534910111437	24.627245108710525	42.16651543127352	17.30089035890159
29	16.616330896022333	22.6526231796487	41.28063043052611	19.450415493802858
30	18.39978567383263	25.19479890605229	38.794510049960316	17.610905370154764
31	23.574509923638406	23.425239537914464	35.2831821330296	17.717068405417525
32	23.719832749796304	23.79678384427035	35.07569840223842	17.407685003694915
33	23.007219296934377	24.092271836320496	33.80590007516153	19.094608791583592
34	18.796752263793827	25.496339822770363	34.59204340420694	21.114864509228866
35	19.835381502683287	25.150954677805455	34.76610446400563	20.247559355505633
36	24.436604298734466	25.6267671908324	31.04645067024298	18.890177840190155
37	20.01665343795594	29.014510176282222	31.702745606629374	19.266090779132465
38	20.578996456670534	29.005246569833908	30.42636801360908	19.98938895988648
39	20.48978161047798	27.941774022742155	30.569901279430372	20.998543087349493
40	22.745259244131823	25.530341469165872	30.61227175210589	21.11212753459641
41	19.032447886950315	24.65682548839207	31.827067415895925	24.483659208761686
42	20.972857633106447	25.320857641527905	29.82712847148388	23.879156253881767
43	20.56857489941618	25.812513026946565	30.41131465313057	23.207597420506676
44	20.141185783732684	27.174631613741298	31.52536859679521	21.158814005730804
45	19.086397867686227	30.456422100438544	28.882030077245847	21.57514995462938
46	20.826166319632318	28.49211646037598	30.14661762569556	20.535099594296145
47	20.527151841036513	26.041050408756632	31.34788631870596	22.083911431500894
48	20.694107293616323	25.47739153685336	32.424938470704895	21.403562698825418
49	20.45867484109757	25.19048290759342	32.90880400524657	21.44203824606244
50	19.470100657505522	27.912930520846274	31.673902104733493	20.943066716914714
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	845.0
1	1734.5
2	2624.0
3	33816.0
4	65008.0
5	45873.5
6	26739.0
7	26680.5
8	26622.0
9	27381.0
10	28140.0
11	28117.5
12	28095.0
13	27176.0
14	26257.0
15	24831.0
16	23405.0
17	22216.0
18	21027.0
19	19127.0
20	17227.0
21	16700.0
22	16173.0
23	15411.0
24	14649.0
25	15908.5
26	17168.0
27	18748.0
28	20328.0
29	23528.5
30	26729.0
31	31334.5
32	35940.0
33	41796.5
34	47653.0
35	53655.5
36	59658.0
37	64039.5
38	68421.0
39	77354.5
40	86288.0
41	104861.5
42	123435.0
43	133877.0
44	144319.0
45	146152.5
46	147986.0
47	146697.0
48	145408.0
49	140657.5
50	135907.0
51	124392.5
52	112878.0
53	103828.5
54	94779.0
55	86773.5
56	78768.0
57	71935.0
58	65102.0
59	59378.5
60	53655.0
61	47944.5
62	42234.0
63	36291.5
64	30349.0
65	26228.0
66	22107.0
67	18383.0
68	14659.0
69	12667.5
70	10676.0
71	9281.0
72	7886.0
73	6459.5
74	5033.0
75	3950.0
76	2867.0
77	2137.0
78	1407.0
79	1014.0
80	621.0
81	503.0
82	385.0
83	283.5
84	182.0
85	143.5
86	105.0
87	86.5
88	68.0
89	61.5
90	55.0
91	35.5
92	16.0
93	16.0
94	16.0
95	9.5
96	3.0
97	3.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1899908.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.514474333351995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.45401199091575	21.03591950605837
2	7.127300087149764	3.922078306279592
3	3.1348968679342866	2.587651182314504
4	1.9047497223840304	2.0963274939197896
5	1.3431479876211587	1.8478005415647876
6	1.0315106070499664	1.702888327335398
7	0.8145834013089542	1.5688983861382855
8	0.721442525911274	1.5880089489739502
9	0.6088818503741434	1.5077757639746916
>10	5.7189586092141225	34.07373417517415
>50	0.9402970787728622	17.743480894361515
>100	0.1970939075175518	7.508314202487178
>500	0.0027346933653968723	0.4980054747969578
>1k	1.9533524038549088E-4	0.06852546733931805
>5k	0.0	0.0
>10k+	1.9533524038549088E-4	2.2505913292815247
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41875	2.204054091040198	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.023948528033989013	0.0
2	0.0	0.0	0.0	0.09005699223330814	0.0
3	0.0	0.0	0.0	0.13663819511260544	0.0
4	0.0	0.0	0.0	0.198009587832674	0.0
5	0.0	0.0	0.0	0.36385972373399134	0.0
6	0.0	0.0	0.0	0.5580270202557176	0.0
7	0.0	0.0	0.0	0.6946652153683232	0.0
8	0.0	0.0	0.0	1.0033117393052717	0.0
9	0.0	0.0	0.0	1.1604772441612963	0.0
10	1.052682550944572E-4	0.0	0.0	1.4141737389389382	0.0
11	1.579023826416858E-4	0.0	0.0	1.5984458194817854	0.0
12	1.579023826416858E-4	0.0	0.0	1.763664345852536	0.0
13	3.158047652833716E-4	0.0	0.0	1.82835168860808	0.0
14	3.158047652833716E-4	0.0	0.0	1.8590373849681143	0.0
15	3.158047652833716E-4	0.0	0.0	1.895828640123627	0.0
16	3.684388928306002E-4	0.0	0.0	1.9659372980165355	0.0
17	4.210730203778288E-4	0.0	0.0	2.0660474086113645	0.0
18	4.737071479250574E-4	0.0	0.0	2.2018960918107613	0.0
19	4.737071479250574E-4	0.0	0.0	2.269688848091592	0.0
20	4.737071479250574E-4	0.0	0.0	2.346903113203376	0.0
21	4.737071479250574E-4	0.0	0.0	2.439907616579329	0.0
22	4.737071479250574E-4	0.0	0.0	2.5447021645258614	0.0
23	4.737071479250574E-4	0.0	0.0	2.6555496371403247	0.0
24	4.737071479250574E-4	0.0	0.0	2.7387115586649458	0.0
25	4.737071479250574E-4	0.0	0.0	2.810346606256724	0.0
26	4.737071479250574E-4	0.0	0.0	2.8811921419352937	0.0
27	4.737071479250574E-4	0.0	0.0	2.961353918189723	0.0
28	6.316095305667432E-4	0.0	0.0	3.0411998896788686	0.0
29	6.316095305667432E-4	0.0	0.0	3.137520343090297	0.0
30	6.316095305667432E-4	0.0	0.0	3.262736932525154	0.0
31	6.316095305667432E-4	0.0	0.0	3.364110262181116	0.0
32	6.316095305667432E-4	0.0	0.0	3.458062179852919	0.0
33	6.316095305667432E-4	0.0	0.0	3.5622777523964317	0.0
34	7.368777856612004E-4	0.0	0.0	3.6690197630622112	0.0
35	7.89511913208429E-4	0.0	0.0	3.7954995715582016	0.0
36	8.421460407556576E-4	0.0	0.0	3.902662655244359	0.0
37	8.947801683028862E-4	0.0	0.0	4.013036420710898	0.0
38	8.947801683028862E-4	0.0	0.0	4.146148129277838	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCG	40	8.3182385E-9	44.000004	9
CGTTTTT	28945	0.0	43.369152	1
TCGCCGT	60	3.6379788E-12	40.333332	25
GTTTTTT	32800	0.0	39.123783	2
ACGTTAG	40	4.126432E-7	38.500004	1
TACGTAG	70	0.0	37.714283	1
CGGGATA	410	0.0	37.560974	6
CGACCCG	510	0.0	37.52941	32
CGTTGAT	295	0.0	37.288136	25
TAGACGG	195	0.0	37.230766	2
ACCCGCT	510	0.0	37.09804	34
GCGTAAG	95	0.0	37.05263	1
TACGGGA	505	0.0	37.029705	4
ATAGGGA	1900	0.0	36.93684	4
TAGGGCG	425	0.0	36.75294	5
AAGGGAT	2300	0.0	36.730434	5
GGGCGAT	2480	0.0	36.637096	7
TTACGGG	415	0.0	36.578312	3
TCAGACG	545	0.0	36.330276	22
AGGGATC	1530	0.0	36.235294	6
>>END_MODULE