FastQCFastQC Report
Thu 26 May 2016
SRR1546071_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546071_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1871928
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT417342.229466090576133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACG302.5285735E-644.01
CGTTTTT273850.043.3010751
TATCGCG557.8216544E-1140.01
TTGATCG501.3496901E-939.61
CGACGGT2350.039.31914528
GTTTTTT308900.039.263842
CGGGTAT2400.038.56
TATAGCG800.038.51
AGTACGG2150.037.8604662
TACGTAG357.2926596E-637.7142871
GGGCGAT25950.037.3872837
ACATACG5650.037.38052717
TCTAGCG651.0913936E-1137.230771
AAGGGAT24200.036.9090925
GCGCGAC5500.036.89
AGGGCGA13850.036.5342986
ACGGGTA1700.036.2352945
AGGGATG31900.036.0689666
GCGATGT2450.035.9183659
TAACGGG6750.035.8518523
Produced by FastQC (version 0.11.5)