##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546071_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1871928
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.896287677731195	31.0	28.0	33.0	19.0	33.0
2	28.753583471159146	31.0	28.0	33.0	16.0	33.0
3	28.138812497061853	31.0	27.0	33.0	16.0	34.0
4	31.95690699642294	35.0	32.0	35.0	25.0	37.0
5	33.63222730788791	35.0	33.0	35.0	28.0	37.0
6	33.534701120983286	35.0	33.0	37.0	28.0	37.0
7	34.27772061745964	35.0	33.0	37.0	31.0	37.0
8	34.80530768277412	35.0	35.0	37.0	32.0	37.0
9	36.73602884298969	39.0	37.0	39.0	32.0	39.0
10	36.36540454547397	39.0	35.0	39.0	32.0	39.0
11	36.42301840669086	39.0	35.0	39.0	32.0	39.0
12	35.483474791765495	37.0	35.0	39.0	30.0	39.0
13	35.84698610202957	37.0	35.0	39.0	31.0	39.0
14	36.62872396801586	38.0	35.0	40.0	31.0	41.0
15	37.00791536853981	39.0	35.0	40.0	32.0	41.0
16	36.579526028778886	38.0	35.0	40.0	31.0	41.0
17	36.22168106893	38.0	34.0	40.0	30.0	41.0
18	35.811562731045214	38.0	34.0	39.0	29.0	41.0
19	35.21919165694407	37.0	34.0	39.0	27.0	41.0
20	35.646377958981326	36.0	34.0	39.0	30.0	41.0
21	35.78668089798325	37.0	34.0	39.0	31.0	41.0
22	36.0965042458898	37.0	35.0	40.0	31.0	41.0
23	36.40276816202333	37.0	35.0	40.0	32.0	41.0
24	35.97946822741046	37.0	35.0	40.0	31.0	41.0
25	35.63607788333739	36.0	34.0	40.0	30.0	41.0
26	35.748888311943624	37.0	34.0	40.0	30.0	41.0
27	36.0997960391639	37.0	35.0	40.0	31.0	41.0
28	36.01784684026309	37.0	35.0	40.0	31.0	41.0
29	36.109164989251724	37.0	35.0	40.0	31.0	41.0
30	35.7356746627007	37.0	35.0	40.0	30.0	41.0
31	34.98066699146548	36.0	34.0	40.0	28.0	41.0
32	34.91860691223167	37.0	34.0	40.0	26.0	41.0
33	34.552366864537525	37.0	34.0	40.0	23.0	41.0
34	34.070671521554246	37.0	34.0	40.0	20.0	41.0
35	33.724723921005506	37.0	33.0	40.0	17.0	41.0
36	33.32007374215247	37.0	33.0	40.0	15.0	41.0
37	33.2434025240287	37.0	33.0	40.0	15.0	41.0
38	33.07299265783727	37.0	33.0	40.0	12.0	41.0
39	32.79483078408999	36.0	32.0	40.0	11.0	41.0
40	32.67171012987679	36.0	32.0	40.0	10.0	41.0
41	32.33932394835699	36.0	31.0	40.0	10.0	40.0
42	32.543174203281325	36.0	32.0	40.0	10.0	41.0
43	32.336569034706464	35.0	32.0	40.0	10.0	40.0
44	32.20476108055438	35.0	31.0	40.0	10.0	40.0
45	32.167596189597035	35.0	31.0	39.0	10.0	40.0
46	31.96758529174199	35.0	31.0	39.0	10.0	40.0
47	31.935380527456186	35.0	31.0	39.0	10.0	40.0
48	31.91456188485882	35.0	31.0	39.0	10.0	40.0
49	31.95305695518204	35.0	31.0	39.0	10.0	40.0
50	32.09547162070336	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	8.0
12	18.0
13	31.0
14	92.0
15	216.0
16	519.0
17	1121.0
18	2216.0
19	3862.0
20	6133.0
21	9008.0
22	12772.0
23	18609.0
24	27464.0
25	42086.0
26	58588.0
27	63250.0
28	57846.0
29	53383.0
30	55631.0
31	65883.0
32	84908.0
33	125334.0
34	192348.0
35	163579.0
36	185039.0
37	265977.0
38	306966.0
39	69034.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.230534507737474	22.164901641516128	25.569626609570456	25.034937241175943
2	40.90990679128684	19.240643870918113	28.132812800492324	11.716636537302717
3	27.490266719660156	18.436446273574624	41.4249907047707	12.648296301994522
4	23.321623481245005	24.410767935518887	38.553031954220465	13.714576629015646
5	19.89403438593792	28.56744490172699	38.897543067895775	12.640977644439316
6	18.41732160638657	35.738340363518255	36.29311597454603	9.551222055549145
7	74.78839998119587	4.254650819903329	18.824121440568227	2.1328277583325854
8	76.14758687299938	3.033663687919621	18.58426178784654	2.234487651234449
9	72.31501425268493	5.089779094067721	19.45892149698065	3.136285156266694
10	37.26334559876234	28.40659469808668	24.62215427089076	9.707905432260215
11	30.894030112269277	21.35114171057861	34.976505506622054	12.778322670530063
12	28.609166591877468	20.55885696458411	37.153619156292336	13.67835728724609
13	19.966419648618963	28.643783307905007	37.03358248821536	14.35621455526067
14	16.376644828219888	30.276484993012552	38.43881815967281	14.90805201909475
15	15.335525725348411	22.18862050249796	47.2199785461834	15.25587522597023
16	17.83738477120915	19.480182998491394	45.51569291126582	17.16673931903364
17	18.063087896543028	19.40747721066195	38.08928548533918	24.44014940745584
18	20.30783235252638	21.506436144979936	40.603164224265036	17.58256727822865
19	23.547647131727288	22.763642618733197	35.99534811167951	17.693362137860003
20	24.286671282228802	22.323882115124086	35.95613720185819	17.433309400788918
21	20.629799864097336	24.3099627763461	37.66613886858896	17.3940984909676
22	20.648817689569256	21.265668337671105	36.73389147445842	21.351622498301218
23	18.084830185776376	25.105506194682704	36.747513793265554	20.06214982627537
24	17.67113906090405	22.38040138295917	43.195571624549665	16.75288793158711
25	18.0533653003748	23.116433965408927	40.3484001521426	18.48180058207367
26	16.940074618254545	27.2972571594634	36.608940087439265	19.153728134842794
27	17.162465650388263	26.63467825685603	37.952634930403306	18.250221162352396
28	15.903977076041386	24.767298742259317	42.03890320567885	17.289820976020444
29	16.636644144432903	22.684045540213084	41.19880679171421	19.480503523639797
30	18.38366646580424	25.22361971186926	38.74075284946857	17.651960972857932
31	23.56340628485711	23.491982597621273	35.181855285032334	17.762755832489283
32	23.76122372227992	23.843919210567925	34.96544738900214	17.429409678150016
33	23.047574479360318	24.15498886709318	33.75001602625742	19.047420627289082
34	18.857028689137618	25.432335004337776	34.58311430781526	21.127521998709355
35	19.91625746289387	25.193704031351633	34.680180006923344	20.209858498831153
36	24.458900128637424	25.595802830023377	31.14136868512037	18.80392835621883
37	19.98837562128458	29.02889427371138	31.758219333222222	19.224510771781823
38	20.541441764854206	29.07115017244253	30.40827424986431	19.979133812838956
39	20.48844827365155	28.056207290023977	30.5603634327816	20.89498100354287
40	22.769679175694794	25.64222555568377	30.59252278933805	20.995572479283393
41	19.0184131013586	24.645499185866125	31.888085439183563	24.44800227359172
42	20.965389694475427	25.32453171275818	29.834480813364618	23.875597779401772
43	20.627930134064986	25.85259689475236	30.420080259497162	23.099392711685493
44	20.09308050309627	27.22551294707916	31.54010196973388	21.141304580090686
45	19.15228577167498	30.543375599916235	28.917191259492885	21.387147368915898
46	20.826442042642665	28.511833788479045	30.23492356543628	20.426800603442015
47	20.52210341423388	26.149777128180148	31.366484181015508	21.961635276570465
48	20.733436328747686	25.47710168339808	32.52961652371245	21.259845464141783
49	20.467026509566608	25.28617553666594	32.91365907235748	21.33313888140997
50	19.50822894897667	27.916992533900874	31.758486437512556	20.816292079609898
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	868.0
1	1720.5
2	2573.0
3	33745.5
4	64918.0
5	45859.0
6	26800.0
7	26387.0
8	25974.0
9	26791.5
10	27609.0
11	27477.0
12	27345.0
13	26341.5
14	25338.0
15	24010.0
16	22682.0
17	21404.0
18	20126.0
19	18370.0
20	16614.0
21	16076.5
22	15539.0
23	14828.5
24	14118.0
25	15576.0
26	17034.0
27	18613.0
28	20192.0
29	23177.0
30	26162.0
31	30952.0
32	35742.0
33	41588.5
34	47435.0
35	53490.5
36	59546.0
37	63960.5
38	68375.0
39	76970.5
40	85566.0
41	104204.0
42	122842.0
43	132712.5
44	142583.0
45	144499.0
46	146415.0
47	144358.5
48	142302.0
49	138135.0
50	133968.0
51	121846.5
52	109725.0
53	101635.5
54	93546.0
55	85758.5
56	77971.0
57	71192.0
58	64413.0
59	58134.5
60	51856.0
61	46728.5
62	41601.0
63	35609.5
64	29618.0
65	25584.0
66	21550.0
67	17932.5
68	14315.0
69	12460.0
70	10605.0
71	9054.0
72	7503.0
73	6164.0
74	4825.0
75	3871.5
76	2918.0
77	2161.5
78	1405.0
79	988.0
80	571.0
81	480.0
82	389.0
83	284.0
84	179.0
85	155.5
86	132.0
87	93.5
88	55.0
89	51.0
90	47.0
91	34.0
92	21.0
93	15.0
94	9.0
95	6.5
96	4.0
97	4.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1871928.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.281449993174395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.34637493346433	20.8283980990745
2	6.983913900732721	3.810625956789505
3	3.121463967618209	2.554741894142157
4	1.9722031913027187	2.152182509596163
5	1.3836316275417304	1.8873738527877109
6	1.0861874754666974	1.7779661577094226
7	0.8820447758978176	1.6844422311777918
8	0.6993737242292347	1.5263943427280002
9	0.5960861523544747	1.46358851013741
>10	5.775602901424249	34.1316769857069
>50	0.9516433561861529	17.86929106169917
>100	0.1980895573906008	7.466821224294609
>500	0.002986267403882081	0.5115754332916423
>1k	1.9908449359213874E-4	0.06821729952376078
>5k	0.0	0.0
>10k+	1.9908449359213874E-4	2.266704441341268
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41734	2.229466090576133	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0684171613438124E-4	0.0	0.0	0.0233449149753623	0.0
2	1.0684171613438124E-4	0.0	0.0	0.08969362069481304	0.0
3	1.0684171613438124E-4	0.0	0.0	0.13510135005192508	0.0
4	1.6026257420157186E-4	0.0	0.0	0.1986721711518819	0.0
5	1.6026257420157186E-4	0.0	0.0	0.36064421281160386	0.0
6	1.6026257420157186E-4	0.0	0.0	0.5491130000726524	0.0
7	1.6026257420157186E-4	0.0	0.0	0.6744917539563487	0.0
8	1.6026257420157186E-4	0.0	0.0	0.9731143505519443	0.0
9	2.671042903359531E-4	0.0	0.0	1.1270732635015877	0.0
10	2.671042903359531E-4	0.0	0.0	1.3653302904812579	0.0
11	3.205251484031437E-4	0.0	0.0	1.5476556790645795	0.0
12	3.205251484031437E-4	0.0	0.0	1.7032706386143057	0.0
13	4.2736686453752494E-4	0.0	0.0	1.7693522400434205	0.0
14	4.2736686453752494E-4	0.0	0.0	1.7992679205610471	0.0
15	4.807877226047156E-4	0.0	0.0	1.8375172549371557	0.0
16	5.342085806719062E-4	0.0	0.0	1.911825668508618	0.0
17	5.342085806719062E-4	0.0	0.0	2.004671119829395	0.0
18	5.876294387390968E-4	0.0	0.0	2.1421764084943438	0.0
19	5.876294387390968E-4	0.0	0.0	2.205800650452368	0.0
20	6.410502968062874E-4	0.0	0.0	2.2824061609207194	0.0
21	6.410502968062874E-4	0.0	0.0	2.37119162702839	0.0
22	6.410502968062874E-4	0.0	0.0	2.46836416785261	0.0
23	6.410502968062874E-4	0.0	0.0	2.572695103657833	0.0
24	6.944711548734781E-4	0.0	0.0	2.65870268514601	0.0
25	7.478920129406686E-4	0.0	0.0	2.731034526968986	0.0
26	7.478920129406686E-4	0.0	0.0	2.7978640204110414	0.0
27	8.013128710078593E-4	0.0	0.0	2.877621361505357	0.0
28	8.547337290750499E-4	0.0	0.0	2.9606907957998385	0.0
29	8.547337290750499E-4	0.0	0.0	3.051078887649525	0.0
30	8.547337290750499E-4	0.0	0.0	3.171222397442637	0.0
31	8.547337290750499E-4	0.0	0.0	3.269730459718536	0.0
32	8.547337290750499E-4	0.0	0.0	3.3664756336782182	0.0
33	9.081545871422405E-4	0.0	0.0	3.4687765768768886	0.0
34	9.081545871422405E-4	0.0	0.0	3.57599223901774	0.0
35	9.615754452094312E-4	0.0	0.0	3.699554683727152	0.0
36	9.615754452094312E-4	0.0	0.0	3.8055950869905253	0.0
37	9.615754452094312E-4	0.0	0.0	3.9146270583056615	0.0
38	0.0010684171613438123	0.0	0.0	4.044973951989607	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGACG	30	2.5285735E-6	44.0	1
CGTTTTT	27385	0.0	43.301075	1
TATCGCG	55	7.8216544E-11	40.0	1
TTGATCG	50	1.3496901E-9	39.6	1
CGACGGT	235	0.0	39.319145	28
GTTTTTT	30890	0.0	39.26384	2
CGGGTAT	240	0.0	38.5	6
TATAGCG	80	0.0	38.5	1
AGTACGG	215	0.0	37.860466	2
TACGTAG	35	7.2926596E-6	37.714287	1
GGGCGAT	2595	0.0	37.387283	7
ACATACG	565	0.0	37.380527	17
TCTAGCG	65	1.0913936E-11	37.23077	1
AAGGGAT	2420	0.0	36.909092	5
GCGCGAC	550	0.0	36.8	9
AGGGCGA	1385	0.0	36.534298	6
ACGGGTA	170	0.0	36.235294	5
AGGGATG	3190	0.0	36.068966	6
GCGATGT	245	0.0	35.918365	9
TAACGGG	675	0.0	35.851852	3
>>END_MODULE