FastQCFastQC Report
Thu 26 May 2016
SRR1546070_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546070_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1674371
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221581.3233626239346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT260300.043.4252781
ATAGGCG2650.040.6792454
GTACCGG650.040.6153832
CGTGTTT4100.039.7073171
TCGACGG1000.039.6000022
GTTTTTT295750.038.9937482
CGTTAGG2900.037.9310382
ACGGCGG2150.037.8604665
CGGAATT3850.037.7142879
TCGATAG1400.037.7142871
TACGTAG357.292201E-637.7142871
CATACGA5200.037.65384318
ACATACG5250.037.2952417
CGGTCTA2150.036.83720831
ACGGGAG4600.036.8260885
TAGGGAC11450.036.698695
ATAGGGA16600.036.5783124
TAACGGG4050.036.395063
TAACGGC1150.036.3478283
ACGGTCT2250.036.1777830
Produced by FastQC (version 0.11.5)