##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546070_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1674371
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.952814519601688	28.0	25.0	30.0	16.0	33.0
2	26.20769829386677	30.0	25.0	31.0	10.0	33.0
3	26.4239472613895	30.0	25.0	31.0	16.0	33.0
4	27.743043208464552	32.0	19.0	35.0	10.0	35.0
5	31.169294618695616	33.0	30.0	35.0	19.0	37.0
6	29.85662197923877	33.0	28.0	35.0	17.0	36.0
7	29.954704781676224	32.0	28.0	35.0	17.0	35.0
8	32.503328712692706	33.0	32.0	35.0	28.0	35.0
9	27.21551436330419	31.0	23.0	34.0	10.0	35.0
10	31.76881527451204	34.0	30.0	37.0	17.0	39.0
11	33.84490175713746	35.0	32.0	37.0	27.0	39.0
12	33.44911133792929	35.0	32.0	39.0	25.0	39.0
13	33.82577218549533	35.0	32.0	39.0	25.0	39.0
14	33.647972880562314	36.0	31.0	39.0	24.0	40.0
15	32.29780616123906	35.0	29.0	38.0	19.0	40.0
16	30.7647253804563	34.0	27.0	38.0	17.0	40.0
17	31.148419316865855	33.0	27.0	38.0	17.0	40.0
18	31.998405371330488	34.0	29.0	38.0	18.0	39.0
19	31.665614132112896	35.0	29.0	37.0	18.0	39.0
20	32.27877453682607	34.0	30.0	37.0	23.0	39.0
21	33.056929437979996	35.0	31.0	37.0	25.0	39.0
22	33.413235776300475	35.0	31.0	38.0	26.0	39.0
23	34.59983898431113	35.0	33.0	38.0	29.0	40.0
24	34.19861607732098	35.0	33.0	38.0	27.0	40.0
25	32.95668403239186	35.0	31.0	38.0	23.0	40.0
26	26.551899788039808	33.0	20.0	36.0	0.0	39.0
27	29.92866455522701	34.0	24.0	37.0	15.0	39.0
28	31.434869571916856	34.0	29.0	37.0	20.0	39.0
29	32.65626435240458	35.0	30.0	37.0	23.0	39.0
30	32.42484013399659	35.0	31.0	38.0	22.0	39.0
31	31.231551430358028	34.0	29.0	37.0	18.0	39.0
32	31.537397028496073	34.0	30.0	37.0	18.0	39.0
33	31.216294357702086	34.0	30.0	37.0	17.0	39.0
34	31.04980676325617	34.0	30.0	38.0	15.0	39.0
35	30.200508728352318	34.0	27.0	37.0	10.0	39.0
36	29.92819631969259	34.0	26.0	37.0	10.0	39.0
37	30.01787417483939	34.0	27.0	38.0	10.0	39.0
38	29.714942506768214	34.0	26.0	37.0	10.0	39.0
39	29.53533416429214	34.0	26.0	37.0	9.0	39.0
40	29.43059214475167	33.0	26.0	37.0	9.0	39.0
41	28.808295174725316	33.0	24.0	36.0	8.0	38.0
42	29.012776738249766	33.0	24.0	37.0	8.0	38.0
43	28.57717913174559	33.0	24.0	36.0	8.0	38.0
44	28.395557495919363	33.0	23.0	36.0	8.0	38.0
45	27.948839295472748	32.0	23.0	35.0	8.0	38.0
46	27.49170046542851	31.0	23.0	35.0	8.0	38.0
47	27.5349740290533	31.0	23.0	35.0	8.0	38.0
48	27.565507286019646	31.0	23.0	35.0	8.0	38.0
49	27.38864863282988	31.0	23.0	35.0	8.0	38.0
50	29.26462653736836	34.0	24.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	13.0
11	37.0
12	63.0
13	197.0
14	500.0
15	1124.0
16	2442.0
17	4505.0
18	7642.0
19	12395.0
20	18099.0
21	25599.0
22	34415.0
23	45755.0
24	58703.0
25	72130.0
26	82854.0
27	88374.0
28	95028.0
29	109770.0
30	130633.0
31	154254.0
32	174448.0
33	181644.0
34	166189.0
35	129286.0
36	64761.0
37	13165.0
38	345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.19722331550176	23.065915499014256	25.303233273868216	26.433627911615766
2	40.88795135606147	16.68937171033182	29.731403613655516	12.691273319951193
3	28.63678360411163	14.247917576212203	43.23277218728705	13.88252663238912
4	24.408449501335127	24.272935926386687	37.640702090516385	13.677912481761808
5	19.104666767401014	28.776179233873496	39.76281242329209	12.356341575433403
6	18.427815579701274	34.19319852051905	37.61537914834884	9.763606751430835
7	76.58870107043182	2.9528700628474813	19.06453229302228	1.3938965736984217
8	78.45734308585133	1.776906073982409	18.442447940151855	1.3233029000143934
9	66.18234548973913	3.4024120102414583	18.021215130935737	12.394027369083673
10	37.538454739122926	28.775880614272463	24.89543834669855	8.790226299906054
11	31.126076598316622	21.0636710740929	35.79057449036086	12.019677837229622
12	28.69059485621765	20.409634423912024	38.008004199786065	12.891766520084257
13	19.325943891765924	29.039024206702095	37.670683498460015	13.964348403071961
14	15.77254981124255	30.28946392406462	39.27444992776392	14.663536336928912
15	13.871119363629687	20.692546633929997	47.20471150061725	18.23162250182307
16	16.09326726275121	17.76488006540964	45.29014179055896	20.851710881280194
17	17.354098942229648	18.72177671495744	39.00437836059034	24.919745982222576
18	19.812454945767694	21.20324587561538	41.59472422778464	17.38957495083228
19	23.737391533895416	22.22297208922037	36.94784489220131	17.091791484682904
20	24.396026925932183	21.94680868218573	36.820035702959494	16.837128688922586
21	20.227715362963167	23.97640666256164	38.52754258166201	17.26833539281318
22	20.192956041403008	20.8610278128324	37.43686435085175	21.509151794912835
23	17.434785958428566	25.005151188117807	37.641538225399266	19.918524628054357
24	16.81891289325962	21.85131013377561	45.076748223661305	16.253028749303468
25	17.498212761687824	22.019612140917395	41.94960376165139	18.53257133574339
26	13.483690293250419	39.25653275170198	30.64105864232001	16.618718312727584
27	16.3476911628307	27.162200014214292	38.77038004122145	17.71972878173356
28	15.261671397796544	24.543783904522954	43.423530388426464	16.771014309254042
29	15.943718566554246	22.460135776360197	42.32293798686193	19.273207670223623
30	17.92243176691426	24.95205662305427	39.60693299155324	17.518578618478223
31	23.842147289937536	23.158427851414054	35.937136990547494	17.062287868100917
32	23.726163436896602	23.21594198657287	35.70051081868952	17.357383757841006
33	22.91188750880181	23.85654075470729	34.49832802885382	18.733243707637076
34	18.376393284403516	24.839178413864072	35.07311103692073	21.71131726481168
35	19.41726176576159	24.869577889249157	34.978388899473295	20.73477144551596
36	24.222349765971817	25.01500563495187	31.424935095029717	19.337709504046593
37	19.317642266857227	29.207624833444918	31.645376084511735	19.82935681518612
38	19.900368556311594	29.356815186120638	30.504290865047235	20.238525392520536
39	19.918345456293736	27.914661684895403	30.598415763292603	21.568577095518258
40	22.378970968799628	25.03997023359817	30.840655983649977	21.740402813952226
41	18.376572456164137	23.9862013854755	32.11187962524434	25.525346533116018
42	20.2845128110795	24.75228010996368	29.3055720625835	25.657635016373316
43	20.100742308604246	25.25712640746883	30.539468254048835	24.102663029878084
44	19.4256828385107	26.956391385182854	31.586966090549822	22.03095968575662
45	18.490525695918052	31.165733281333708	28.454685371402157	21.889055651346087
46	19.997121307046047	28.257297815119824	29.894270744058517	21.85131013377561
47	19.987386308052397	25.50408481752252	30.916803981913212	23.591724892511877
48	20.05875639269911	24.887674237071714	32.95798840280917	22.095580967420002
49	19.99156698246685	25.04379256449138	32.42345931696141	22.541181136080354
50	18.873714367962656	28.26530082042749	31.701815189106835	21.15916962250302
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	517.0
1	1024.0
2	1531.0
3	19918.0
4	38305.0
5	30977.0
6	23649.0
7	23374.0
8	23099.0
9	24249.0
10	25399.0
11	26177.5
12	26956.0
13	26874.5
14	26793.0
15	26208.0
16	25623.0
17	24492.5
18	23362.0
19	21860.0
20	20358.0
21	19549.0
22	18740.0
23	17856.0
24	16972.0
25	17409.5
26	17847.0
27	19373.0
28	20899.0
29	23104.5
30	25310.0
31	29666.5
32	34023.0
33	39082.5
34	44142.0
35	48879.5
36	53617.0
37	57049.5
38	60482.0
39	67945.0
40	75408.0
41	90242.0
42	105076.0
43	113887.0
44	122698.0
45	124724.5
46	126751.0
47	125277.0
48	123803.0
49	118981.5
50	114160.0
51	105434.5
52	96709.0
53	89808.5
54	82908.0
55	75834.0
56	68760.0
57	63401.0
58	58042.0
59	52820.5
60	47599.0
61	42466.5
62	37334.0
63	32235.5
64	27137.0
65	23382.5
66	19628.0
67	16522.0
68	13416.0
69	11679.0
70	9942.0
71	8518.5
72	7095.0
73	5868.5
74	4642.0
75	3685.5
76	2729.0
77	2053.0
78	1377.0
79	1018.5
80	660.0
81	521.0
82	382.0
83	303.0
84	224.0
85	180.0
86	136.0
87	100.0
88	64.0
89	52.5
90	41.0
91	28.0
92	15.0
93	11.5
94	8.0
95	5.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1674371.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.718313971681404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.82728997902547	34.528055508041476
2	7.4042913904501875	6.325976887101372
3	2.9697312300006464	3.805857332840256
4	1.6781701828654345	2.867544030782385
5	1.121375983801212	2.395164567816165
6	0.7780477021842304	1.994213161611073
7	0.611454275436585	1.82842070021989
8	0.48255560657126384	1.649116952824513
9	0.40171701957681966	1.5444606393045601
>10	3.361879578547164	29.50724231297393
>50	0.31877950170301594	9.111364501878402
>100	0.04394462956116056	2.822827208880888
>500	6.103362214836067E-4	0.1754681560922256
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	1.5258405537090167E-4	1.444288039632814
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22158	1.3233626239346	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01815607174276191	0.0
2	0.0	0.0	0.0	0.07083256936485402	0.0
3	5.972392020645365E-5	0.0	0.0	0.10696554108975848	0.0
4	5.972392020645365E-5	0.0	0.0	0.14811532211200504	0.0
5	5.972392020645365E-5	0.0	0.0	0.2530502499147441	0.0
6	5.972392020645365E-5	0.0	0.0	0.36138944116925104	0.0
7	5.972392020645365E-5	0.0	0.0	0.4347304151827761	0.0
8	5.972392020645365E-5	0.0	0.0	0.6162911326103951	0.0
9	5.972392020645365E-5	0.0	0.0	0.7326333291725668	0.0
10	5.972392020645365E-5	0.0	0.0	0.8938879137299918	0.0
11	1.194478404129073E-4	0.0	0.0	1.0227124096153122	0.0
12	1.194478404129073E-4	0.0	0.0	1.1283640244605289	0.0
13	1.194478404129073E-4	0.0	0.0	1.1701707686050462	0.0
14	1.194478404129073E-4	0.0	0.0	1.1889838034700793	0.0
15	1.194478404129073E-4	0.0	0.0	1.2132317150738994	0.0
16	1.194478404129073E-4	0.0	0.0	1.2564718333033718	0.0
17	1.194478404129073E-4	0.0	0.0	1.327961365790497	0.0
18	1.194478404129073E-4	0.0	0.0	1.4132471238453126	0.0
19	1.194478404129073E-4	0.0	0.0	1.451589880617856	0.0
20	1.194478404129073E-4	0.0	0.0	1.499787084224464	0.0
21	1.194478404129073E-4	0.0	0.0	1.5563456366599755	0.0
22	1.194478404129073E-4	0.0	0.0	1.6152931459037454	0.0
23	1.194478404129073E-4	0.0	0.0	1.6796158079660959	0.0
24	1.194478404129073E-4	0.0	0.0	1.729783900939517	0.0
25	1.194478404129073E-4	0.0	0.0	1.7712920254830022	0.0
26	1.194478404129073E-4	0.0	0.0	1.813516837068965	0.0
27	1.194478404129073E-4	0.0	0.0	1.869060082860967	0.0
28	1.194478404129073E-4	0.0	0.0	1.9272311811420528	0.0
29	1.194478404129073E-4	0.0	0.0	1.9941816956934872	0.0
30	1.194478404129073E-4	0.0	0.0	2.080004969030161	0.0
31	1.194478404129073E-4	0.0	0.0	2.1582433045006155	0.0
32	1.194478404129073E-4	0.0	0.0	2.2271049844986566	0.0
33	1.194478404129073E-4	0.0	0.0	2.3019390565173428	0.0
34	1.194478404129073E-4	0.0	0.0	2.3838802750405974	0.0
35	1.194478404129073E-4	0.0	0.0	2.480871921455878	0.0
36	1.194478404129073E-4	0.0	0.0	2.5622756246972744	0.0
37	1.194478404129073E-4	0.0	0.0	2.64385849969929	0.0
38	1.194478404129073E-4	0.0	0.0	2.742343244119732	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	26030	0.0	43.425278	1
ATAGGCG	265	0.0	40.679245	4
GTACCGG	65	0.0	40.615383	2
CGTGTTT	410	0.0	39.707317	1
TCGACGG	100	0.0	39.600002	2
GTTTTTT	29575	0.0	38.993748	2
CGTTAGG	290	0.0	37.931038	2
ACGGCGG	215	0.0	37.860466	5
CGGAATT	385	0.0	37.714287	9
TCGATAG	140	0.0	37.714287	1
TACGTAG	35	7.292201E-6	37.714287	1
CATACGA	520	0.0	37.653843	18
ACATACG	525	0.0	37.29524	17
CGGTCTA	215	0.0	36.837208	31
ACGGGAG	460	0.0	36.826088	5
TAGGGAC	1145	0.0	36.69869	5
ATAGGGA	1660	0.0	36.578312	4
TAACGGG	405	0.0	36.39506	3
TAACGGC	115	0.0	36.347828	3
ACGGTCT	225	0.0	36.17778	30
>>END_MODULE