FastQCFastQC Report
Thu 26 May 2016
SRR1546069_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546069_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences637571
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91681.437957498066882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG302.525936E-644.0000042
CTCACGG254.4406017E-544.02
ACACGTG207.853842E-444.042
GTACCAC207.853842E-444.09
TAGACTA207.853842E-444.01
CGCATTG207.853842E-444.012
GGCGTAA207.853842E-444.030
TTCGTAC351.4447323E-744.033
ACGATTG207.853842E-444.01
GTGTCGA207.853842E-444.010
ATAACGG454.783942E-1044.02
ACGTAAG207.853842E-444.01
TCGAGAG254.4406017E-544.01
CACGTGA207.853842E-444.043
CGTTTTT62850.043.1599041
ACGGGAT1700.042.705885
CGTTAGG1150.042.086962
CGACGGT900.041.55555728
GTTGTAG750.041.0666661
GCGTTAG501.3460522E-939.6000021
Produced by FastQC (version 0.11.5)