##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546069_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	637571
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.973314344598485	31.0	28.0	33.0	23.0	33.0
2	28.71422006333412	31.0	28.0	33.0	16.0	33.0
3	28.25198919022352	31.0	27.0	33.0	16.0	34.0
4	32.307178337785125	35.0	32.0	35.0	26.0	37.0
5	33.68920481013095	35.0	33.0	35.0	28.0	37.0
6	33.4863615189524	35.0	33.0	37.0	28.0	37.0
7	34.53123652110902	35.0	35.0	37.0	32.0	37.0
8	34.94439678090754	35.0	35.0	37.0	32.0	37.0
9	36.6824228203604	39.0	37.0	39.0	32.0	39.0
10	36.331326236607374	38.0	35.0	39.0	32.0	39.0
11	36.37546877132115	38.0	35.0	39.0	32.0	39.0
12	35.04084564699461	37.0	34.0	39.0	29.0	39.0
13	35.09412128217877	37.0	34.0	39.0	27.0	39.0
14	35.766004099935536	38.0	33.0	40.0	27.0	41.0
15	36.419797951914376	38.0	35.0	40.0	31.0	41.0
16	36.04827697621128	37.0	34.0	40.0	31.0	41.0
17	35.60593878956226	37.0	34.0	40.0	29.0	41.0
18	35.26501675891783	37.0	33.0	39.0	29.0	40.0
19	34.76015220265665	36.0	33.0	39.0	27.0	40.0
20	35.257731295808625	35.0	34.0	39.0	30.0	40.0
21	35.47728174587615	36.0	34.0	39.0	30.0	40.0
22	35.79580156562955	36.0	34.0	40.0	31.0	41.0
23	36.15571912775204	37.0	35.0	40.0	32.0	41.0
24	35.855634901838386	36.0	35.0	40.0	31.0	41.0
25	35.51752667546046	36.0	34.0	40.0	30.0	41.0
26	35.63991304497852	36.0	34.0	40.0	31.0	41.0
27	35.90675234601323	36.0	35.0	40.0	31.0	41.0
28	35.83229005083356	36.0	35.0	40.0	31.0	41.0
29	35.98332577861917	36.0	35.0	40.0	31.0	41.0
30	35.62217541262071	36.0	35.0	40.0	30.0	41.0
31	34.94528138826892	36.0	34.0	40.0	29.0	41.0
32	34.894948170478266	36.0	34.0	40.0	28.0	41.0
33	34.63830538089091	36.0	34.0	40.0	26.0	41.0
34	34.40657746352955	36.0	34.0	40.0	24.0	41.0
35	34.20161832956643	36.0	34.0	40.0	23.0	41.0
36	33.844354589528066	36.0	33.0	40.0	21.0	41.0
37	33.769658594885904	36.0	33.0	40.0	21.0	41.0
38	33.611260549805436	35.0	33.0	40.0	20.0	41.0
39	33.42462408108273	35.0	33.0	40.0	18.0	40.0
40	33.2262022582583	35.0	33.0	39.0	18.0	40.0
41	32.95677971551404	35.0	33.0	39.0	18.0	40.0
42	33.13879395392827	35.0	33.0	39.0	18.0	40.0
43	32.918021679154165	35.0	33.0	39.0	18.0	40.0
44	32.79235724335015	35.0	32.0	39.0	18.0	40.0
45	32.69241543294786	35.0	32.0	39.0	18.0	40.0
46	32.56343215108592	35.0	32.0	39.0	18.0	40.0
47	32.52956298200514	35.0	32.0	38.0	18.0	40.0
48	32.54709357859752	35.0	32.0	38.0	18.0	40.0
49	32.557354396608375	35.0	32.0	38.0	18.0	40.0
50	32.70521871289629	35.0	32.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	5.0
13	15.0
14	29.0
15	73.0
16	136.0
17	334.0
18	643.0
19	1123.0
20	1725.0
21	2555.0
22	3687.0
23	5137.0
24	7491.0
25	11455.0
26	15830.0
27	18041.0
28	17556.0
29	17420.0
30	19527.0
31	24983.0
32	33871.0
33	52776.0
34	85376.0
35	59778.0
36	61550.0
37	85026.0
38	91991.0
39	19436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.088995578531645	22.945679775272087	26.746354523653054	21.218970122543215
2	36.24835508515915	20.994524531385526	30.246670566885886	12.51044981656945
3	29.108914928690293	18.838372510669398	38.236996350210404	13.815716210429896
4	25.504139931082186	25.388074426220765	34.65857135911138	14.44921428358567
5	20.851952174738187	29.525339138699845	35.29144205115979	14.331266635402176
6	19.163042233727694	37.00638830812568	32.92025515589636	10.91031430225026
7	78.99669213311145	4.652971982728198	14.060551687576755	2.289784196583596
8	80.97702059849021	2.8214896850703686	13.76929000848533	2.4321997079540947
9	77.45709889565241	4.119070660365669	15.080987058696207	3.3428433852857173
10	47.78338412506215	19.720940883446705	20.56021995981624	11.935455031674904
11	41.55850877784592	20.017221611397005	25.21899521778751	13.205274392969567
12	37.04685438955034	19.78273792252157	29.450994477477803	13.719413210450288
13	21.27261120722241	33.60849223066921	30.34454201963389	14.774354542474486
14	16.2554444916723	36.000382702475484	33.232377256807474	14.511795549044734
15	15.20191476713966	21.742990192464838	47.78024721952536	15.274847820870146
16	17.0914298172282	16.93693721954104	47.44240249321252	18.52923047001824
17	17.857775839867244	17.208122703196977	33.763141673633214	31.170959783302564
18	21.34444634401502	21.13850849552442	37.73524830960003	19.78179685086053
19	26.333067219180293	22.847024096139883	31.203113065054715	19.61679561962511
20	28.135846831176448	21.58928182116188	30.3374839821761	19.93738736548557
21	22.79761783393536	24.757399568048108	33.00244208096039	19.442540517056138
22	22.13369177707267	21.416124635530785	31.494061053592464	24.956122533804077
23	19.521120000752855	26.40051068822139	30.806294514650133	23.27207479637562
24	19.38309615713387	22.342295995269545	40.35283913477872	17.92176871281787
25	18.2458110547688	21.94218369405133	39.08521560735981	20.726789643820062
26	17.305994155944983	29.02249318115159	32.74756850609579	20.923944156807632
27	17.559142432764354	28.116711707401997	34.62092849266983	19.703217367163813
28	15.916501848421587	24.659057579469582	41.848829385276304	17.575611186832525
29	16.350649574714033	22.895646131960206	40.33495877321898	20.41874552010678
30	18.92197104322499	25.524529817071357	36.98192044493868	18.571578694764977
31	27.084356095242722	23.00982949349955	30.61698226550455	19.28883214575318
32	26.568491979716768	24.30788100462537	31.0464246334918	18.077202382166067
33	25.60405037242911	24.109942265253594	30.60459148863421	19.68141587368309
34	19.67294622873374	25.166922585876712	32.298520478503576	22.86161070688598
35	19.671220930688506	24.188992284780834	34.173135227292335	21.966651557238333
36	28.52717579689164	22.71903835023864	29.16835928861256	19.58542656425716
37	20.729456013526338	28.954579176279978	30.184873527811018	20.13109128238267
38	21.361228788636875	29.711671327585478	27.142075157119756	21.785024726657895
39	21.11184479846166	28.32139479367788	28.033897401230607	22.53286300662985
40	23.282112894093363	24.786729634817142	28.808556223542165	23.12260124754733
41	19.034115416165413	23.712182643187973	29.685948702183758	27.567753238462856
42	22.12114415492549	25.522177137918757	26.406313963464463	25.95036474369129
43	21.69860297911919	25.287693449043324	27.86779825305731	25.14590531878018
44	20.34879252663625	27.5900252677741	30.41104441701395	21.650137788575705
45	19.0450945855442	32.25413326515792	26.596096748440566	22.104675400857314
46	22.28065580147152	28.66065112748227	28.876783918967458	20.18190915207875
47	21.872544391134475	25.69094265579833	29.777389498581336	22.65912345448585
48	22.425580837271454	23.80315290375503	31.897153415070633	21.874112843902875
49	21.200148689322443	23.261723008104195	32.950212603772755	22.587915698800604
50	20.031808222143102	28.82079015513566	29.838088620718324	21.309313002002913
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	200.0
1	529.0
2	858.0
3	7774.5
4	14691.0
5	10408.0
6	6125.0
7	6262.5
8	6400.0
9	6601.0
10	6802.0
11	6835.0
12	6868.0
13	6502.5
14	6137.0
15	5761.5
16	5386.0
17	4978.0
18	4570.0
19	4218.0
20	3866.0
21	3742.0
22	3618.0
23	3399.5
24	3181.0
25	3353.0
26	3525.0
27	3821.5
28	4118.0
29	5002.5
30	5887.0
31	6910.5
32	7934.0
33	9006.0
34	10078.0
35	12633.5
36	15189.0
37	15935.0
38	16681.0
39	20885.0
40	25089.0
41	34178.5
42	43268.0
43	50399.0
44	57530.0
45	58058.0
46	58586.0
47	56098.5
48	53611.0
49	52101.0
50	50591.0
51	48258.5
52	45926.0
53	40839.5
54	35753.0
55	32644.0
56	29535.0
57	27475.0
58	25415.0
59	23316.0
60	21217.0
61	19574.0
62	17931.0
63	15718.0
64	13505.0
65	11694.5
66	9884.0
67	8111.0
68	6338.0
69	5337.0
70	4336.0
71	3623.5
72	2911.0
73	2415.5
74	1920.0
75	1502.5
76	1085.0
77	824.0
78	563.0
79	401.5
80	240.0
81	174.5
82	109.0
83	75.5
84	42.0
85	39.5
86	37.0
87	27.0
88	17.0
89	11.5
90	6.0
91	7.0
92	8.0
93	5.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	637571.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.16573550963291
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.79732398830646	21.348873797925936
2	7.5326465278934736	4.243250595844046
3	3.3784511270462017	2.8546968262981354
4	2.0800330682567836	2.3434264500724318
5	1.4030723764330275	1.9759282727742375
6	1.0101250001320023	1.7070548151231533
7	0.7491675511411724	1.4770598568489153
8	0.5981954042104132	1.3478890830454755
9	0.4734250006006271	1.2000927015508558
>10	5.902908304848988	38.54303952110026
>50	1.0021154545333	18.480197483343392
>100	0.07142025511150446	2.9507716402382385
>500	5.579707430586286E-4	0.08690757973219779
>1k	0.0	0.0
>5k	5.579707430586286E-4	1.4408113761026933
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9168	1.437957498066882	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.030271138430072887	0.0
2	0.0	0.0	0.0	0.1041452638215979	0.0
3	0.0	0.0	0.0	0.14241551137049835	0.0
4	0.0	0.0	0.0	0.21456433871678604	0.0
5	0.0	0.0	0.0	0.38976051294679337	0.0
6	0.0	0.0	0.0	0.55899656665689	0.0
7	0.0	0.0	0.0	0.6758462979025082	0.0
8	0.0	0.0	0.0	0.9918895307346163	0.0
9	0.0	0.0	0.0	1.132736589336717	0.0
10	0.0	0.0	0.0	1.3687887309805495	0.0
11	0.0	0.0	0.0	1.5675116967365204	0.0
12	0.0	0.0	0.0	1.7576081722663044	0.0
13	1.568452768397559E-4	0.0	0.0	1.8416772406524136	0.0
14	1.568452768397559E-4	0.0	0.0	1.8699093904835697	0.0
15	1.568452768397559E-4	0.0	0.0	1.90817963803247	0.0
16	1.568452768397559E-4	0.0	0.0	1.991150789480701	0.0
17	1.568452768397559E-4	0.0	0.0	2.0945118269181	0.0
18	1.568452768397559E-4	0.0	0.0	2.2624931184134787	0.0
19	1.568452768397559E-4	0.0	0.0	2.3358967079744843	0.0
20	3.136905536795118E-4	0.0	0.0	2.4143193463943624	0.0
21	3.136905536795118E-4	0.0	0.0	2.520503598814877	0.0
22	3.136905536795118E-4	0.0	0.0	2.618845587393404	0.0
23	3.136905536795118E-4	0.0	0.0	2.730048888672791	0.0
24	3.136905536795118E-4	0.0	0.0	2.8086283723695087	0.0
25	3.136905536795118E-4	0.0	0.0	2.8782676752863603	0.0
26	3.136905536795118E-4	0.0	0.0	2.9428879293443395	0.0
27	3.136905536795118E-4	0.0	0.0	3.0086061003401974	0.0
28	3.136905536795118E-4	0.0	0.0	3.0816959993475237	0.0
29	3.136905536795118E-4	0.0	0.0	3.1588638755526834	0.0
30	4.705358305192677E-4	0.0	0.0	3.26395021103532	0.0
31	4.705358305192677E-4	0.0	0.0	3.3593121393538916	0.0
32	4.705358305192677E-4	0.0	0.0	3.4427538266326416	0.0
33	4.705358305192677E-4	0.0	0.0	3.5393705171659313	0.0
34	4.705358305192677E-4	0.0	0.0	3.631909230501387	0.0
35	4.705358305192677E-4	0.0	0.0	3.7576991425268713	0.0
36	4.705358305192677E-4	0.0	0.0	3.8502378558623276	0.0
37	4.705358305192677E-4	0.0	0.0	3.947011391672457	0.0
38	4.705358305192677E-4	0.0	0.0	4.058685228782363	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGG	30	2.525936E-6	44.000004	2
CTCACGG	25	4.4406017E-5	44.0	2
ACACGTG	20	7.853842E-4	44.0	42
GTACCAC	20	7.853842E-4	44.0	9
TAGACTA	20	7.853842E-4	44.0	1
CGCATTG	20	7.853842E-4	44.0	12
GGCGTAA	20	7.853842E-4	44.0	30
TTCGTAC	35	1.4447323E-7	44.0	33
ACGATTG	20	7.853842E-4	44.0	1
GTGTCGA	20	7.853842E-4	44.0	10
ATAACGG	45	4.783942E-10	44.0	2
ACGTAAG	20	7.853842E-4	44.0	1
TCGAGAG	25	4.4406017E-5	44.0	1
CACGTGA	20	7.853842E-4	44.0	43
CGTTTTT	6285	0.0	43.159904	1
ACGGGAT	170	0.0	42.70588	5
CGTTAGG	115	0.0	42.08696	2
CGACGGT	90	0.0	41.555557	28
GTTGTAG	75	0.0	41.066666	1
GCGTTAG	50	1.3460522E-9	39.600002	1
>>END_MODULE