FastQCFastQC Report
Thu 26 May 2016
SRR1546068_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546068_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences638614
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94641.4819593682568812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA207.8538497E-444.00000444
ACGCATT207.8538497E-444.00000411
TAGCACG207.8538497E-444.0000041
CTAGTAG408.301868E-944.0000041
TACCGAA207.8538497E-444.00000422
TCCGATT254.4406108E-544.01
ACGTCGA254.4406108E-544.014
ACGTATG254.4406108E-544.01
ATAACGG502.7284841E-1144.02
TAATGCG302.5259433E-644.01
CCCGCAT254.4406108E-544.042
TGCGTAG454.783942E-1044.01
TAATCGG254.4406108E-544.02
CGTTTTT65500.043.093131
TTTACGG900.041.5555572
AATAGCG750.041.0666661
GCATAGG1450.040.9655152
ATAGCGG1350.040.740742
ACGTAGG650.040.6153832
TGCATAG603.6379788E-1240.3333321
Produced by FastQC (version 0.11.5)