##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546068_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	638614
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.943319438659348	31.0	28.0	33.0	23.0	33.0
2	28.6580328649231	31.0	28.0	33.0	16.0	33.0
3	28.229958942334495	31.0	27.0	33.0	16.0	34.0
4	32.287671425931784	35.0	32.0	35.0	26.0	37.0
5	33.729373299050756	35.0	33.0	35.0	28.0	37.0
6	33.56861421766513	35.0	33.0	37.0	28.0	37.0
7	34.58929494185846	35.0	35.0	37.0	32.0	37.0
8	34.9761827958673	35.0	35.0	37.0	32.0	37.0
9	36.78736137948745	39.0	37.0	39.0	32.0	39.0
10	36.33054709104404	38.0	35.0	39.0	32.0	39.0
11	36.42214232697686	38.0	35.0	39.0	32.0	39.0
12	34.979533802891886	37.0	33.0	39.0	29.0	39.0
13	35.102615977726764	37.0	34.0	39.0	27.0	39.0
14	35.764295803098584	38.0	34.0	40.0	27.0	41.0
15	36.44422139195195	38.0	35.0	40.0	31.0	41.0
16	36.07453328614781	37.0	34.0	40.0	31.0	41.0
17	35.654234013034475	37.0	34.0	40.0	30.0	41.0
18	35.34249797217098	37.0	33.0	39.0	29.0	40.0
19	34.84098532133652	36.0	33.0	39.0	27.0	40.0
20	35.23806242894769	35.0	34.0	39.0	29.0	40.0
21	35.387803900321636	36.0	34.0	39.0	30.0	40.0
22	35.78082534989837	36.0	34.0	40.0	31.0	40.0
23	36.18099509249719	37.0	35.0	40.0	32.0	41.0
24	35.72445483500205	36.0	34.0	40.0	31.0	41.0
25	35.41367868540308	35.0	34.0	40.0	30.0	41.0
26	35.606518178430164	36.0	34.0	40.0	30.0	41.0
27	35.92657066710094	36.0	35.0	40.0	31.0	41.0
28	35.85003147441177	36.0	35.0	40.0	31.0	41.0
29	36.010405346578686	37.0	35.0	40.0	31.0	41.0
30	35.65601286536155	36.0	35.0	40.0	30.0	41.0
31	34.97528240846584	36.0	34.0	40.0	29.0	41.0
32	34.96402208532854	36.0	34.0	40.0	29.0	41.0
33	34.730705872404926	36.0	34.0	40.0	27.0	41.0
34	34.44093302057268	36.0	34.0	40.0	24.0	41.0
35	34.26210198962127	36.0	34.0	40.0	23.0	41.0
36	33.88214320387589	36.0	33.0	40.0	21.0	41.0
37	33.80792309595468	36.0	33.0	40.0	21.0	41.0
38	33.67503687673618	35.0	33.0	40.0	21.0	41.0
39	33.456380536599575	35.0	33.0	40.0	19.0	40.0
40	33.24655269067073	35.0	33.0	39.0	18.0	40.0
41	32.97033106070334	35.0	33.0	39.0	18.0	40.0
42	33.14283902325975	35.0	33.0	39.0	18.0	40.0
43	32.952141669302584	35.0	33.0	39.0	18.0	40.0
44	32.81900803928508	35.0	32.0	39.0	18.0	40.0
45	32.720942541190766	35.0	32.0	39.0	18.0	40.0
46	32.55614659246431	35.0	32.0	39.0	18.0	40.0
47	32.542662703918175	35.0	32.0	38.0	18.0	40.0
48	32.568966543170056	35.0	32.0	38.0	18.0	40.0
49	32.633258901308146	35.0	32.0	38.0	18.0	40.0
50	32.80417591847345	35.0	33.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	0.0
11	1.0
12	10.0
13	9.0
14	31.0
15	67.0
16	146.0
17	358.0
18	620.0
19	1031.0
20	1649.0
21	2405.0
22	3491.0
23	5084.0
24	7494.0
25	11370.0
26	15463.0
27	17771.0
28	17341.0
29	17366.0
30	19908.0
31	24754.0
32	33644.0
33	53664.0
34	85299.0
35	60604.0
36	62452.0
37	86938.0
38	92368.0
39	17273.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.161903747803837	22.895677200938284	26.720836060593722	21.221582990664157
2	36.2863952246584	21.008308618351617	30.150607409170483	12.554688747819498
3	29.008916184111218	18.820288938231858	38.25706921551986	13.913725662137066
4	25.319206907458963	25.463895248146766	34.6201304700492	14.596767374345065
5	20.915921041505513	29.55243699637026	35.18573034728333	14.34591161484089
6	19.27345783211768	37.01531754706286	32.932569596031406	10.778655024788057
7	78.97337045539246	4.6438067439799315	14.083155082726028	2.2996677179015808
8	81.06446147438045	2.819543574052558	13.720181518100135	2.3958134334668517
9	77.56328549013959	4.083687485711243	15.081254090890587	3.2717729332585885
10	47.84204542963355	19.5302639779272	20.455705637521255	12.171984954917994
11	41.5647010557238	20.009896431960463	25.15745035342163	13.267952158894106
12	37.02220746804799	19.38213067674683	29.528322272922296	14.067339582282882
13	21.471342626375247	33.51774311242785	30.30202908173012	14.708885179466783
14	16.358394898953048	36.006727068307306	33.175126132530764	14.45975190020889
15	15.169883529017527	21.792350308637143	47.88448107933744	15.153285083007889
16	17.181427278449892	16.9736335251028	47.439611408456436	18.40532778799087
17	17.842233336569507	17.126307910568826	33.83499265597059	31.19646609689108
18	21.356562806327453	21.128725646478156	37.677846085428754	19.836865461765637
19	26.297262509121317	22.84619504113596	31.258005618417386	19.59853683132534
20	28.056697786143115	21.361260479726408	30.362785657689933	20.21925607644054
21	22.803289624092173	24.763628733475933	33.016188182532794	19.416893459899093
22	22.249903697695324	21.40776744637606	31.384999389302457	24.957329466626163
23	19.550620562656	26.465595805917193	30.77398866921176	23.209794962215046
24	19.349246962954147	22.422308311436957	40.26328893510008	17.96515579050882
25	18.23730766942159	21.984171972427788	39.094194615213574	20.684325742937048
26	17.24077455239002	28.956458831156223	32.867115346672634	20.93565126978112
27	17.581512462927527	28.095218708014546	34.529778551675975	19.793490277381956
28	15.865295781176108	24.773024080273842	41.86049789074464	17.501182247805403
29	16.418211940233068	22.96739501482899	40.28364551982888	20.330747525109064
30	18.91847031227001	25.420833241989683	37.03598731001826	18.624709135722046
31	27.166645266154514	23.029247714581892	30.45392052162965	19.35018649763394
32	26.60856166635871	24.1150366261936	31.16264284841861	18.113758859029087
33	25.684842487010933	24.085754462006783	30.612388704287724	19.61701434669456
34	19.594622103492874	25.124879817855543	32.38857901643246	22.891919062219117
35	19.664774026250598	24.155280028311314	34.28769178251651	21.892254162921574
36	28.522550398206114	22.83069271891941	29.2380060568669	19.408750826007573
37	20.768570685891635	28.860939472044144	30.13353919582095	20.236950646243272
38	21.27435352184575	29.835080345874033	27.174787900046038	21.715778232234182
39	21.02991791598681	28.360010898602283	27.983100902892826	22.626970282518077
40	23.169551560097336	24.69895742968366	29.005001456278755	23.126489553940253
41	18.97672146241705	23.719649115114922	29.686790455580365	27.616838966887663
42	21.939074307797824	25.81496804016198	26.314800489810747	25.93115716222945
43	21.61540461060985	25.32813248691698	27.995314853730108	25.06114804874306
44	20.434096339886064	27.526173870287842	30.303594972863106	21.736134816962984
45	19.102305931282434	32.16512635175552	26.596817482861322	22.135750234100723
46	22.30799825872906	28.63670386180071	28.871274353521848	20.184023525948383
47	21.73660458430288	25.679518457158785	29.9735051220299	22.61037183650844
48	22.421525365870462	23.815325063340296	31.826267510577594	21.936882060211644
49	21.202009351501847	23.188342253693154	33.13566567597954	22.473982718825454
50	20.01725612028549	28.70685578455843	29.911026065823798	21.364862029332272
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	202.0
1	528.5
2	855.0
3	7999.0
4	15143.0
5	10617.5
6	6092.0
7	6150.0
8	6208.0
9	6596.5
10	6985.0
11	6929.5
12	6874.0
13	6486.5
14	6099.0
15	5743.5
16	5388.0
17	4973.5
18	4559.0
19	4190.0
20	3821.0
21	3656.5
22	3492.0
23	3320.5
24	3149.0
25	3274.5
26	3400.0
27	3676.0
28	3952.0
29	4912.5
30	5873.0
31	6894.5
32	7916.0
33	9115.0
34	10314.0
35	12785.0
36	15256.0
37	15950.5
38	16645.0
39	20715.5
40	24786.0
41	33845.5
42	42905.0
43	50109.0
44	57313.0
45	58193.0
46	59073.0
47	56685.0
48	54297.0
49	52431.5
50	50566.0
51	48217.5
52	45869.0
53	40911.0
54	35953.0
55	32638.5
56	29324.0
57	27392.0
58	25460.0
59	23602.0
60	21744.0
61	19783.0
62	17822.0
63	15747.5
64	13673.0
65	11782.0
66	9891.0
67	8175.5
68	6460.0
69	5358.5
70	4257.0
71	3621.0
72	2985.0
73	2457.5
74	1930.0
75	1503.0
76	1076.0
77	812.0
78	548.0
79	392.5
80	237.0
81	168.0
82	99.0
83	72.0
84	45.0
85	39.5
86	34.0
87	27.5
88	21.0
89	15.0
90	9.0
91	9.0
92	9.0
93	6.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	638614.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.951956436272724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.20271339721486	21.02062968768453
2	7.845935415648307	4.386184898800217
3	3.498653407859577	2.93382622926324
4	2.108206399378564	2.3571397373640397
5	1.421826853777192	1.9871421138351393
6	1.0267080423001764	1.721909908268723
7	0.7756413172351057	1.5176484616670205
8	0.6357836079698764	1.4217116570296224
9	0.47300488185252976	1.1899270666517616
>10	5.938944262936014	38.65391280115646
>50	1.0049471591461274	18.46913535167501
>100	0.06650800043704026	2.7592397620871236
>500	5.636271223477989E-4	0.09058826442846273
>1k	0.0	0.0
>5k	5.636271223477989E-4	1.4910040600886456
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9464	1.4819593682568812	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02646356014744431	0.0
2	0.0	0.0	0.0	0.09833796315144985	0.0
3	0.0	0.0	0.0	0.1365457067962807	0.0
4	0.0	0.0	0.0	0.2137441396524348	0.0
5	0.0	0.0	0.0	0.3814510799951144	0.0
6	0.0	0.0	0.0	0.5546386393032411	0.0
7	0.0	0.0	0.0	0.6683223355579426	0.0
8	0.0	0.0	0.0	0.9904261416129305	0.0
9	0.0	0.0	0.0	1.124623011709734	0.0
10	0.0	0.0	0.0	1.3607593945638523	0.0
11	0.0	0.0	0.0	1.55868803377314	0.0
12	0.0	0.0	0.0	1.737512801160012	0.0
13	1.5658911329848705E-4	0.0	0.0	1.8079779021443314	0.0
14	1.5658911329848705E-4	0.0	0.0	1.835224407858268	0.0
15	3.131782265969741E-4	0.0	0.0	1.8692042454440398	0.0
16	3.131782265969741E-4	0.0	0.0	1.9550150795316106	0.0
17	3.131782265969741E-4	0.0	0.0	2.0577375378554184	0.0
18	3.131782265969741E-4	0.0	0.0	2.2302987407103507	0.0
19	4.697673398954611E-4	0.0	0.0	2.295283222729223	0.0
20	7.829455664924352E-4	0.0	0.0	2.371855299132183	0.0
21	9.395346797909222E-4	0.0	0.0	2.458292489672948	0.0
22	9.395346797909222E-4	0.0	0.0	2.556317274597801	0.0
23	9.395346797909222E-4	0.0	0.0	2.657317252675325	0.0
24	9.395346797909222E-4	0.0	0.0	2.7343590964181805	0.0
25	9.395346797909222E-4	0.0	0.0	2.8040412518360074	0.0
26	0.0010961237930894093	0.0	0.0	2.8720009270075506	0.0
27	0.0012527129063878964	0.0	0.0	2.9471637013908243	0.0
28	0.0012527129063878964	0.0	0.0	3.0201342281879193	0.0
29	0.0012527129063878964	0.0	0.0	3.106101651388789	0.0
30	0.0012527129063878964	0.0	0.0	3.214931085131237	0.0
31	0.0012527129063878964	0.0	0.0	3.310293855130016	0.0
32	0.0012527129063878964	0.0	0.0	3.3895279464590504	0.0
33	0.0012527129063878964	0.0	0.0	3.4801930430588746	0.0
34	0.0012527129063878964	0.0	0.0	3.574303100151265	0.0
35	0.0015658911329848703	0.0	0.0	3.6934674153714138	0.0
36	0.0015658911329848703	0.0	0.0	3.782566620838253	0.0
37	0.0015658911329848703	0.0	0.0	3.8773030343838375	0.0
38	0.0015658911329848703	0.0	0.0	3.986445646352883	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCTA	20	7.8538497E-4	44.000004	44
ACGCATT	20	7.8538497E-4	44.000004	11
TAGCACG	20	7.8538497E-4	44.000004	1
CTAGTAG	40	8.301868E-9	44.000004	1
TACCGAA	20	7.8538497E-4	44.000004	22
TCCGATT	25	4.4406108E-5	44.0	1
ACGTCGA	25	4.4406108E-5	44.0	14
ACGTATG	25	4.4406108E-5	44.0	1
ATAACGG	50	2.7284841E-11	44.0	2
TAATGCG	30	2.5259433E-6	44.0	1
CCCGCAT	25	4.4406108E-5	44.0	42
TGCGTAG	45	4.783942E-10	44.0	1
TAATCGG	25	4.4406108E-5	44.0	2
CGTTTTT	6550	0.0	43.09313	1
TTTACGG	90	0.0	41.555557	2
AATAGCG	75	0.0	41.066666	1
GCATAGG	145	0.0	40.965515	2
ATAGCGG	135	0.0	40.74074	2
ACGTAGG	65	0.0	40.615383	2
TGCATAG	60	3.6379788E-12	40.333332	1
>>END_MODULE