FastQCFastQC Report
Thu 26 May 2016
SRR1546067_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546067_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences629394
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93661.4880980752914708No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTCG207.853763E-444.0000041
GACACGA207.853763E-444.00000425
CTATACG207.853763E-444.00000416
ACGTACA207.853763E-444.00000428
CACACGT207.853763E-444.00000425
ATCGGGT207.853763E-444.0000044
CAATCTA207.853763E-444.00000430
CGATCTA207.853763E-444.00000410
GTCGGAC207.853763E-444.00000410
TCGTTGA700.044.024
ATTCGAT254.4405373E-544.014
TATGCGT254.4405373E-544.020
GTTGTAG502.7284841E-1144.01
ACGTAGG700.044.02
TGCGTTA254.4405373E-544.022
CGTTTTT65200.043.223931
TACGGGA1100.042.04
AATCGTT750.041.06666622
TCACGAC700.040.85714325
GTTGATC1800.040.33333216
Produced by FastQC (version 0.11.5)