##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546067_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	629394
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.843470068033696	31.0	28.0	33.0	16.0	33.0
2	28.552474284788225	31.0	28.0	33.0	16.0	33.0
3	28.124634171917748	31.0	26.0	33.0	16.0	34.0
4	32.18596777217451	35.0	32.0	35.0	25.0	37.0
5	33.6639481787243	35.0	33.0	35.0	28.0	37.0
6	33.503166537971445	35.0	33.0	37.0	28.0	37.0
7	34.52350514939767	35.0	35.0	37.0	32.0	37.0
8	34.95549369711183	35.0	35.0	37.0	32.0	37.0
9	36.7871762361891	39.0	37.0	39.0	32.0	39.0
10	36.413032218292514	39.0	35.0	39.0	32.0	39.0
11	36.429061923056146	38.0	35.0	39.0	32.0	39.0
12	35.15416575308948	37.0	34.0	39.0	30.0	39.0
13	35.18470624124158	37.0	34.0	39.0	27.0	39.0
14	35.850548305195154	38.0	34.0	40.0	27.0	41.0
15	36.42231575134177	38.0	35.0	40.0	31.0	41.0
16	36.03428218254384	37.0	34.0	40.0	31.0	41.0
17	35.60448780890825	37.0	34.0	40.0	30.0	41.0
18	35.2674572684201	37.0	33.0	39.0	29.0	40.0
19	34.719001134424545	36.0	33.0	39.0	27.0	40.0
20	35.3174529785794	35.0	34.0	39.0	30.0	40.0
21	35.4439858022161	36.0	34.0	39.0	30.0	40.0
22	35.78789279846964	36.0	34.0	40.0	31.0	41.0
23	36.16711312786585	37.0	35.0	40.0	31.0	41.0
24	35.73224244273062	36.0	34.0	40.0	31.0	41.0
25	35.43240323231553	35.0	34.0	40.0	30.0	41.0
26	35.611076686463484	36.0	34.0	40.0	30.0	41.0
27	35.92836601556418	36.0	35.0	40.0	31.0	41.0
28	35.85684801571035	36.0	35.0	40.0	31.0	41.0
29	36.00480779924816	36.0	35.0	40.0	31.0	41.0
30	35.66576262245906	36.0	35.0	40.0	30.0	41.0
31	34.94731122317658	36.0	34.0	40.0	29.0	41.0
32	34.954708815146	36.0	34.0	40.0	29.0	41.0
33	34.733915480605155	36.0	34.0	40.0	26.0	41.0
34	34.454357683740234	36.0	34.0	40.0	24.0	41.0
35	34.237943164377164	36.0	34.0	40.0	23.0	41.0
36	33.895289754907104	36.0	33.0	40.0	21.0	41.0
37	33.845912099575145	36.0	33.0	40.0	21.0	41.0
38	33.69363069873561	35.0	33.0	40.0	21.0	41.0
39	33.452557539474476	35.0	33.0	40.0	19.0	41.0
40	33.287115860653266	35.0	33.0	40.0	18.0	40.0
41	32.98406244737001	35.0	33.0	39.0	18.0	40.0
42	33.19734220535944	35.0	33.0	39.0	18.0	40.0
43	32.99757067909767	35.0	33.0	39.0	18.0	40.0
44	32.865715275328334	35.0	33.0	39.0	18.0	40.0
45	32.77916058939233	35.0	32.0	39.0	18.0	40.0
46	32.63046676644518	35.0	32.0	39.0	18.0	40.0
47	32.62388106654973	35.0	32.0	39.0	18.0	40.0
48	32.631520160662475	35.0	32.0	39.0	18.0	40.0
49	32.71046117376397	35.0	32.0	39.0	18.0	40.0
50	32.81258798145518	35.0	33.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	6.0
12	5.0
13	8.0
14	20.0
15	63.0
16	138.0
17	336.0
18	611.0
19	1080.0
20	1633.0
21	2517.0
22	3501.0
23	4982.0
24	7347.0
25	11218.0
26	15580.0
27	17742.0
28	17090.0
29	17302.0
30	19309.0
31	24182.0
32	33063.0
33	52560.0
34	84089.0
35	59011.0
36	60346.0
37	84097.0
38	91970.0
39	19584.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.998846509499614	22.976863459136883	26.7188756168632	21.305414414500298
2	36.42757954476846	20.943637848470114	30.109756368824613	12.519026237936808
3	28.987406934289172	18.84161590355167	38.25616386555957	13.914813296599588
4	25.39013717957273	25.44828835355914	34.64109921607133	14.5204752507968
5	20.901057207409032	29.420363079406542	35.32540824984032	14.353171463344106
6	19.282198432142664	37.06501809677245	32.87733915480605	10.775444316278833
7	78.9082832057503	4.7350308391881715	14.05526585890555	2.301420096155985
8	81.02222137484628	2.8198552893735878	13.78246376673435	2.3754595690457805
9	77.58590008802118	4.1362008535194175	15.076247946437366	3.20165111202204
10	47.86381821243927	19.668284095495032	20.47604521174336	11.991852480322342
11	41.63369844644214	19.976517094220792	25.20456184838114	13.185222610955934
12	37.04309224428577	19.787128571292385	29.46087824161018	13.708900942811658
13	21.36753766321255	33.563872550421515	30.331874787494	14.73671499887193
14	16.183821262992655	36.085981118345586	33.25532178571769	14.47487583294407
15	15.16236252649374	21.64161081929602	47.975512953730096	15.220513700480145
16	17.11424004677515	16.881158701862425	47.55065984105345	18.453941410308964
17	17.81189525162299	17.16063387957305	33.82094522667836	31.206525642125598
18	21.35562143903501	21.125082221946826	37.71310180904172	19.806194529976455
19	26.345659475622586	22.813690629399073	31.175702342253025	19.66494755272532
20	28.119746931175065	21.502588203891364	30.32488393597651	20.05278092895706
21	22.769362275458615	24.70249160303403	32.9750521930619	19.553093928445456
22	22.27285293472769	21.40455739965745	31.503319065640916	24.819270599973944
23	19.514326479121184	26.382996978045547	30.969631105476058	23.133045437357204
24	19.311115136146835	22.279684903256147	40.42634661277356	17.982853347823465
25	18.252636663203017	21.936974295910034	39.18435828749559	20.62603075339136
26	17.30982500627588	29.029510926383157	32.85588995128648	20.804774116054492
27	17.617263590056467	28.054763788660203	34.589938893602415	19.73803372768091
28	15.890999914203185	24.680565750547352	41.89426654845772	17.53416778679174
29	16.36177021071062	22.980676650873697	40.2882772953031	20.36927584311258
30	18.926618302684805	25.409838670212935	37.02879277527272	18.634750251829537
31	27.16104697534454	22.894562070817326	30.5657505473519	19.378640406486237
32	26.5669834793468	24.225524869954274	31.210021067884348	17.997470582814582
33	25.666911346469778	24.201851304588224	30.55542315306469	19.575814195877303
34	19.722939843722692	25.1462517914057	32.449467265337766	22.681341099533835
35	19.694499788685622	24.121933161104174	34.3868864336171	21.796680616593104
36	28.47945801834781	22.746165359059667	29.22017051322383	19.554206109368693
37	20.78030613574327	28.838533573564412	30.176646107207887	20.20451418348443
38	21.409641655306533	29.780709698535418	27.105279046193644	21.70436959996441
39	21.131755307486248	28.285938537704524	28.129438793506132	22.452867361303095
40	23.271750286783796	24.640686120299844	29.00679065895131	23.08077293396505
41	19.009078573993396	23.712173932385756	29.672987031970433	27.605760461650412
42	22.014191428580506	25.758269065164267	26.379342669297767	25.848196836957456
43	21.79842832947248	25.257787649707495	27.944657877259715	24.999126143560314
44	20.427585900088022	27.58161024731726	30.3309214895598	21.659882363034917
45	19.03783639500853	32.2508635290454	26.62068592964026	22.09061414630581
46	22.327349799966317	28.670594254155585	28.917021770147155	20.085034175730943
47	21.834653650972204	25.7002767741669	29.918620133016837	22.54644944184406
48	22.486232789000212	23.899020327489616	31.858581429120708	21.75616545438946
49	21.157653234698774	23.36565013330283	33.13901943774488	22.33767719425352
50	20.082809813884467	28.8164488380887	29.844262894148976	21.256478453877858
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	204.0
1	525.5
2	847.0
3	7984.0
4	15121.0
5	10645.5
6	6170.0
7	6266.5
8	6363.0
9	6641.0
10	6919.0
11	6844.0
12	6769.0
13	6415.0
14	6061.0
15	5592.0
16	5123.0
17	4722.5
18	4322.0
19	4013.0
20	3704.0
21	3551.0
22	3398.0
23	3228.5
24	3059.0
25	3182.5
26	3306.0
27	3573.5
28	3841.0
29	4765.0
30	5689.0
31	6818.0
32	7947.0
33	8923.5
34	9900.0
35	12385.5
36	14871.0
37	15595.0
38	16319.0
39	20595.0
40	24871.0
41	33765.5
42	42660.0
43	49607.5
44	56555.0
45	57422.0
46	58289.0
47	55781.0
48	53273.0
49	51453.0
50	49633.0
51	47663.5
52	45694.0
53	40675.0
54	35656.0
55	32281.5
56	28907.0
57	26941.0
58	24975.0
59	23069.5
60	21164.0
61	19283.0
62	17402.0
63	15453.5
64	13505.0
65	11483.0
66	9461.0
67	7929.0
68	6397.0
69	5353.0
70	4309.0
71	3573.5
72	2838.0
73	2338.5
74	1839.0
75	1435.0
76	1031.0
77	775.0
78	519.0
79	386.5
80	254.0
81	185.5
82	117.0
83	81.5
84	46.0
85	41.5
86	37.0
87	25.5
88	14.0
89	9.5
90	5.0
91	5.5
92	6.0
93	4.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	629394.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.697229251207496
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.1786766566101	20.822410421605312
2	7.652673229379649	4.239156896374131
3	3.5856498537637083	2.979376980427561
4	2.174681207356941	2.409305757938316
5	1.3955771857624162	1.9326810625908315
6	0.9979020772918838	1.6583473563005693
7	0.7764806333351877	1.5054453477425238
8	0.591715433990791	1.311110242137654
9	0.49884331887097577	1.2434919986882356
>10	6.044978389808916	38.80299660980796
>50	1.0360540839734738	18.801665586463194
>100	0.06561675865165331	2.713349909541564
>500	5.755856022074851E-4	0.08752227381541237
>1k	0.0	0.0
>5k	5.755856022074851E-4	1.4931395565667618
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9366	1.4880980752914708	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02637457617962675	0.0
2	0.0	0.0	0.0	0.09564755939840545	0.0
3	0.0	0.0	0.0	0.13505054067881167	0.0
4	0.0	0.0	0.0	0.20861336460150556	0.0
5	0.0	0.0	0.0	0.3660664067340966	0.0
6	0.0	0.0	0.0	0.5252671617460605	0.0
7	0.0	0.0	0.0	0.6387096159162623	0.0
8	0.0	0.0	0.0	0.9434471888832751	0.0
9	0.0	0.0	0.0	1.0670581543516462	0.0
10	0.0	0.0	0.0	1.3020460951327786	0.0
11	0.0	0.0	0.0	1.4904813201269793	0.0
12	0.0	0.0	0.0	1.659056171491943	0.0
13	0.0	0.0	0.0	1.732460112425603	0.0
14	0.0	0.0	0.0	1.7588346886052297	0.0
15	0.0	0.0	0.0	1.7942655951597886	0.0
16	0.0	0.0	0.0	1.8735482066877027	0.0
17	1.588829890338961E-4	0.0	0.0	1.9750744366803623	0.0
18	1.588829890338961E-4	0.0	0.0	2.1366584365278345	0.0
19	1.588829890338961E-4	0.0	0.0	2.2022771109988337	0.0
20	1.588829890338961E-4	0.0	0.0	2.271073445250511	0.0
21	1.588829890338961E-4	0.0	0.0	2.3622722809559673	0.0
22	1.588829890338961E-4	0.0	0.0	2.4577609573653385	0.0
23	1.588829890338961E-4	0.0	0.0	2.563894794039981	0.0
24	1.588829890338961E-4	0.0	0.0	2.646990597304709	0.0
25	3.177659780677922E-4	0.0	0.0	2.714515867644115	0.0
26	3.177659780677922E-4	0.0	0.0	2.7761624673892666	0.0
27	3.177659780677922E-4	0.0	0.0	2.850360823268096	0.0
28	3.177659780677922E-4	0.0	0.0	2.9185216255636375	0.0
29	3.177659780677922E-4	0.0	0.0	2.999869715948992	0.0
30	4.766489671016883E-4	0.0	0.0	3.1050502546894316	0.0
31	4.766489671016883E-4	0.0	0.0	3.1960902074058537	0.0
32	4.766489671016883E-4	0.0	0.0	3.278073829747344	0.0
33	4.766489671016883E-4	0.0	0.0	3.3635528778475803	0.0
34	6.355319561355844E-4	0.0	0.0	3.4539572986078673	0.0
35	6.355319561355844E-4	0.0	0.0	3.570418529569713	0.0
36	6.355319561355844E-4	0.0	0.0	3.66082295033	0.0
37	6.355319561355844E-4	0.0	0.0	3.7517040200573883	0.0
38	6.355319561355844E-4	0.0	0.0	3.8572023247758955	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTTCG	20	7.853763E-4	44.000004	1
GACACGA	20	7.853763E-4	44.000004	25
CTATACG	20	7.853763E-4	44.000004	16
ACGTACA	20	7.853763E-4	44.000004	28
CACACGT	20	7.853763E-4	44.000004	25
ATCGGGT	20	7.853763E-4	44.000004	4
CAATCTA	20	7.853763E-4	44.000004	30
CGATCTA	20	7.853763E-4	44.000004	10
GTCGGAC	20	7.853763E-4	44.000004	10
TCGTTGA	70	0.0	44.0	24
ATTCGAT	25	4.4405373E-5	44.0	14
TATGCGT	25	4.4405373E-5	44.0	20
GTTGTAG	50	2.7284841E-11	44.0	1
ACGTAGG	70	0.0	44.0	2
TGCGTTA	25	4.4405373E-5	44.0	22
CGTTTTT	6520	0.0	43.22393	1
TACGGGA	110	0.0	42.0	4
AATCGTT	75	0.0	41.066666	22
TCACGAC	70	0.0	40.857143	25
GTTGATC	180	0.0	40.333332	16
>>END_MODULE