FastQCFastQC Report
Thu 26 May 2016
SRR1546066_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546066_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences546322
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48500.8877548405519089No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATAGT207.852851E-444.028
GGCCGTA207.852851E-444.09
CACGACG850.044.026
TACGGGA900.044.04
TAGCACG207.852851E-444.01
TGGCACG207.852851E-444.01
AAACGAC207.852851E-444.044
TGTTCGG302.5252684E-644.02
CAGGCGA351.444223E-744.05
TATTATC750.044.044
CTCGAAT600.044.022
AACTCGA302.5252684E-644.013
ACGTAAG207.852851E-444.01
TTTGACG408.29823E-944.01
CGACTAT207.852851E-444.015
AGTCGTT207.852851E-444.036
CTAATAC302.5252684E-644.024
TACGTAG454.783942E-1044.01
CGTTTTT58800.043.4013631
ACGGGAT1100.042.05
Produced by FastQC (version 0.11.5)