FastQCFastQC Report
Thu 26 May 2016
SRR1546065_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546065_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences707658
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120631.7046369856625658No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA9670.13664792880176582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGCG650.044.0000045
CGACGGT254.441102E-544.028
TTCGTAC207.8544294E-444.035
TTGTACG600.044.01
CGATTCC254.441102E-544.013
TACGGGT302.5263325E-644.04
TCGATAG351.4450234E-744.01
ATCCGAT254.441102E-544.020
GCCGAAT207.8544294E-444.019
AAGTTCG207.8544294E-444.02
CGGATGG351.4450234E-744.02
GATATCG302.5263325E-644.033
TATTACG408.305506E-944.01
GACGGTC254.441102E-544.029
TCGTACA207.8544294E-444.036
TCGTAAT254.441102E-544.023
CCGACCG302.5263325E-644.015
CGTAATA254.441102E-544.024
TCGAGTC302.5263325E-644.015
CACGTCT254.441102E-544.044
Produced by FastQC (version 0.11.5)