FastQCFastQC Report
Thu 26 May 2016
SRR1546064_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546064_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences712375
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121181.7010703632216178No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA9970.13995437796104582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAG207.854465E-444.034
GGTACTT408.305506E-944.09
TCGTTCA351.4450416E-744.016
ACGTTAG302.5263562E-644.01
GATCCGC207.854465E-444.018
CACGCGA207.854465E-444.016
CGTTCAC207.854465E-444.021
GCACCTA207.854465E-444.043
ATCGTAG207.854465E-444.01
GTTACGT302.5263562E-644.025
TATCTCG254.4411314E-544.016
CGGATGG351.4450416E-744.02
GATATCG207.854465E-444.033
TATTACG254.4411314E-544.01
GTTCCGC207.854465E-444.020
TATAGCG207.854465E-444.01
CCGAATT207.854465E-444.020
ACCCGGC254.4411314E-544.039
ACATCGG207.854465E-444.023
TACAGCG207.854465E-444.01
Produced by FastQC (version 0.11.5)