FastQCFastQC Report
Thu 26 May 2016
SRR1546063_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546063_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences698517
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121301.7365361186628243No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA10820.15489959442647783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACG650.044.0000041
ACAACGA302.5262852E-644.013
CTATGCG254.4410423E-544.01
CGGAAAT207.854358E-444.033
TTGGACG207.854358E-444.037
CGACGGT254.4410423E-544.028
GACACGC254.4410423E-544.035
CGGGGTA502.7284841E-1144.06
AGTGCTA254.4410423E-544.028
ACGATCT207.854358E-444.040
TCGGACG207.854358E-444.01
GTTCGCC207.854358E-444.034
GTATGCG302.5262852E-644.01
TATTACG254.4410423E-544.01
GCTAGCG351.444987E-744.01
TCGTACA207.854358E-444.036
CATCGGT207.854358E-444.031
CCGGTCA207.854358E-444.031
GGACCGT207.854358E-444.08
CGCGTAA254.4410423E-544.031
Produced by FastQC (version 0.11.5)