##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546063_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	698517
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.98115292827519	31.0	28.0	33.0	23.0	33.0
2	28.58628637527791	31.0	28.0	33.0	16.0	33.0
3	28.1120115902691	31.0	26.0	33.0	16.0	34.0
4	31.94372363163674	35.0	32.0	35.0	25.0	37.0
5	33.59201565602555	35.0	33.0	35.0	28.0	37.0
6	33.4920653326977	35.0	33.0	37.0	28.0	37.0
7	34.283376066724216	35.0	33.0	37.0	31.0	37.0
8	34.74363830801541	35.0	35.0	37.0	32.0	37.0
9	36.69448130825735	39.0	37.0	39.0	32.0	39.0
10	36.482984666085436	39.0	35.0	39.0	32.0	39.0
11	36.42837182201722	39.0	35.0	39.0	32.0	39.0
12	35.22736311356775	37.0	34.0	39.0	30.0	39.0
13	34.9932084687989	37.0	33.0	39.0	27.0	39.0
14	35.75945467325777	38.0	33.0	40.0	27.0	41.0
15	36.40063448706331	38.0	35.0	40.0	31.0	41.0
16	36.070172952125716	37.0	34.0	40.0	31.0	41.0
17	35.614546245832244	37.0	34.0	40.0	30.0	41.0
18	35.262035140161224	37.0	33.0	39.0	29.0	40.0
19	34.625619705748036	36.0	33.0	39.0	27.0	40.0
20	35.11099801436472	35.0	34.0	39.0	30.0	40.0
21	35.23361206670704	35.0	34.0	39.0	30.0	40.0
22	35.55863493658708	35.0	34.0	39.0	31.0	40.0
23	35.91877076721111	35.0	35.0	40.0	31.0	41.0
24	35.518847787527	35.0	34.0	40.0	31.0	41.0
25	35.17954895872255	35.0	34.0	39.0	30.0	41.0
26	35.27015663183573	35.0	34.0	39.0	30.0	41.0
27	35.59782081180558	35.0	34.0	40.0	31.0	41.0
28	35.56789741695621	36.0	35.0	40.0	31.0	41.0
29	35.71691598057026	36.0	35.0	40.0	31.0	41.0
30	35.3635101221588	36.0	34.0	40.0	30.0	41.0
31	34.5908775305397	35.0	34.0	39.0	28.0	41.0
32	34.452720549392495	35.0	34.0	39.0	26.0	41.0
33	34.09722598018373	35.0	33.0	40.0	23.0	41.0
34	33.68717439947775	35.0	33.0	40.0	20.0	41.0
35	33.38681520993762	35.0	33.0	40.0	18.0	41.0
36	32.97400063276914	35.0	33.0	40.0	15.0	41.0
37	32.87983542275993	35.0	33.0	40.0	15.0	41.0
38	32.742902463361666	35.0	33.0	40.0	15.0	41.0
39	32.52896923052696	35.0	32.0	39.0	14.0	40.0
40	32.366775611760346	35.0	32.0	39.0	13.0	40.0
41	32.076659551592876	35.0	31.0	39.0	12.0	40.0
42	32.290097449310466	35.0	32.0	39.0	10.0	40.0
43	32.07506044949515	35.0	31.0	39.0	10.0	40.0
44	31.904948626876653	35.0	31.0	39.0	10.0	40.0
45	31.852428788418894	35.0	31.0	39.0	10.0	40.0
46	31.681450845147648	35.0	31.0	38.0	10.0	40.0
47	31.68063483064836	35.0	31.0	38.0	10.0	40.0
48	31.719301033475205	35.0	31.0	38.0	10.0	40.0
49	31.76959472711473	35.0	31.0	38.0	10.0	40.0
50	31.858049267233294	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	4.0
12	5.0
13	7.0
14	37.0
15	85.0
16	221.0
17	436.0
18	865.0
19	1520.0
20	2321.0
21	3325.0
22	4909.0
23	6993.0
24	10455.0
25	15828.0
26	21719.0
27	23988.0
28	22316.0
29	20709.0
30	22588.0
31	27481.0
32	37274.0
33	58541.0
34	96652.0
35	62617.0
36	59379.0
37	83629.0
38	94282.0
39	20330.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.983762743068528	22.128165814146257	27.530754441194706	24.357317001590513
2	37.49844313023162	20.91302001239769	29.265858955472808	12.322677901897878
3	26.508588910506113	18.653948293312833	42.622012062698545	12.215450733482507
4	24.000847509795754	25.14541521537772	37.99191716164388	12.861820113182642
5	19.82127850861182	28.74590024294326	38.97242300473718	12.46039824370774
6	17.780383297757965	35.93198161247329	36.277427750505716	10.010207339263038
7	74.27879350108874	5.236665678859642	18.056969264885463	2.427571555166159
8	76.30408422414916	3.6681999149627	17.525414556839706	2.502301304048434
9	73.2156840849972	4.842831312623744	18.601408412393685	3.340076189985355
10	46.37052498364392	23.15348087448122	22.171113945687793	8.304880196187064
11	41.82403577865678	17.076320261353697	30.21386737903301	10.885776580956511
12	37.598655437161874	18.899611605730428	32.300001288443944	11.201731668663754
13	18.699043831431446	37.27239279788466	32.26020268654879	11.768360684135104
14	12.76175096669086	38.58245397034002	35.917808729064575	12.737986333904544
15	11.863419215280373	20.53121112299343	54.646916252575096	12.958453409151103
16	13.445771541709078	16.140194154186656	53.149887547475586	17.264146756628687
17	14.199224929386114	16.48993510537324	37.21269489504193	32.09814507019872
18	19.008986180722875	20.06307648919067	42.41399994559904	18.513937384487424
19	24.812424035492338	21.427109146949896	34.652986255166304	19.107480562391466
20	27.071352594138727	20.29972069398454	33.68436272846617	18.944563983410568
21	19.27870044680373	25.252928704670037	36.716357654860225	18.75201319366601
22	20.434148345709552	21.76138877078153	33.942910480346214	23.8615524031627
23	16.32845013077706	27.145080219951694	33.9013939531894	22.625075696081844
24	16.99972942677129	20.530495320801066	47.044524327969114	15.425250924458531
25	14.5723010320436	22.12530260537682	44.29613023018767	19.00626613239191
26	14.363716273190203	30.387234670022345	36.78307041918808	18.46597863759937
27	14.817821184022723	30.685437863359088	37.21040432802638	17.286336624591815
28	13.431026016546483	25.30919075698945	46.13760295025032	15.12218027621375
29	13.674541922386998	21.35409732332928	44.924747715517306	20.046613038766413
30	16.34834943172464	26.691834271750007	38.988027492530605	17.971788803994748
31	25.838025416704248	23.527702260646485	33.06161482111387	17.572657501535396
32	26.707295599104963	23.79770284760428	33.62437850474648	15.870623048544271
33	25.314774014089853	24.553160481419923	31.86135770496638	18.270707799523848
34	17.748172199101813	25.22515557960651	34.51870176387976	22.507970457411915
35	19.034755059647797	23.770788685171585	35.90019999513254	21.294256260048073
36	27.70354909043015	22.357651997016536	31.497443870371082	18.441355042182224
37	19.79536646924842	29.82461414682821	32.70342740405746	17.676591979865915
38	19.23818604271621	30.769043559426613	29.215036999815325	20.77773339804185
39	20.06794394409871	29.124416442262678	31.271823019339546	19.535816594299067
40	23.30122244698411	25.097742789366617	28.801160172193374	22.7998745914559
41	17.27488378951407	22.81590856056474	31.362443576892186	28.546764073029003
42	20.83098908115336	24.019458366797085	28.105543601658944	27.044008950390612
43	21.249160721929456	24.947138008094292	29.206304213068545	24.597397056907706
44	18.79610660871532	29.05913528231954	30.339848564888182	21.80490954407695
45	16.798302689841478	34.852265585519035	26.19549703156831	22.15393469307118
46	21.252167091137366	29.951597455752687	29.15161692557232	19.644618527537627
47	20.64029937710893	25.87753769772246	30.689589516074772	22.792573409093837
48	21.257893508676236	22.81905809021112	34.015063341336	21.907985059776642
49	19.88312381803163	22.689784214271093	34.957202186918856	22.46988978077842
50	18.765899756197772	29.943437310759798	30.803831545975257	20.486831387067173
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	257.0
1	511.5
2	766.0
3	9564.0
4	18362.0
5	13417.0
6	8472.0
7	8804.5
8	9137.0
9	9925.0
10	10713.0
11	10845.5
12	10978.0
13	10362.5
14	9747.0
15	9248.0
16	8749.0
17	8145.5
18	7542.0
19	6859.5
20	6177.0
21	5786.5
22	5396.0
23	5150.5
24	4905.0
25	5030.5
26	5156.0
27	5773.5
28	6391.0
29	6925.5
30	7460.0
31	9455.5
32	11451.0
33	12471.0
34	13491.0
35	15947.5
36	18404.0
37	18262.5
38	18121.0
39	24442.5
40	30764.0
41	44139.5
42	57515.0
43	64651.0
44	71787.0
45	69818.5
46	67850.0
47	62763.5
48	57677.0
49	55028.5
50	52380.0
51	50173.5
52	47967.0
53	41807.0
54	35647.0
55	30962.0
56	26277.0
57	22640.0
58	19003.0
59	17132.0
60	15261.0
61	13638.5
62	12016.0
63	10047.0
64	8078.0
65	6824.5
66	5571.0
67	4411.0
68	3251.0
69	2610.5
70	1970.0
71	1781.0
72	1592.0
73	1273.5
74	955.0
75	795.0
76	635.0
77	469.0
78	303.0
79	204.5
80	106.0
81	117.5
82	129.0
83	85.0
84	41.0
85	33.0
86	25.0
87	19.5
88	14.0
89	16.5
90	19.0
91	12.5
92	6.0
93	3.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	698517.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.83318205143843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.88414146736098	21.677634883810434
2	7.095818118980908	3.949983950189821
3	3.115075787352425	2.601074144802239
4	2.003665421671777	2.230735378062509
5	1.3882977092412194	1.9320371441452902
6	0.9395805597547354	1.5690910062987582
7	0.7132358118002147	1.3896135536808598
8	0.5475743205177146	1.2192588599729801
9	0.4494037881378797	1.1257503704862903
>10	4.481157347556723	28.614747709609006
>50	1.0956091454165329	21.272033003761813
>100	0.28540644090131695	10.516716051590091
>500	0.0	0.0
>1k	5.170406538067337E-4	0.1557093935032017
>5k	0.0	0.0
>10k+	5.170406538067337E-4	1.7456145500867253
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12130	1.7365361186628243	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1082	0.15489959442647783	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0347879865486452	0.0
2	0.0	0.0	0.0	0.1548995944264778	0.0
3	0.0	0.0	0.0	0.19870668859884585	0.0
4	0.0	0.0	0.0	0.2909020109746792	0.0
5	0.0	0.0	0.0	0.5236808839298113	0.0
6	0.0	0.0	0.0	0.6887448694877862	0.0
7	0.0	0.0	0.0	0.822456719020439	0.0
8	0.0	0.0	0.0	1.1746313976610447	0.0
9	0.0	0.0	0.0	1.3229456119178202	0.0
10	0.0	0.0	0.0	1.6377554161172885	0.0
11	0.0	0.0	0.0	1.840756917870288	0.0
12	0.0	0.0	0.0	2.081839096256784	0.0
13	0.0	0.0	0.0	2.1575709682083613	0.0
14	0.0	0.0	0.0	2.1867756976566066	0.0
15	0.0	0.0	0.0	2.239315578575754	0.0
16	0.0	0.0	0.0	2.3392415646290643	0.0
17	1.4316043847179094E-4	0.0	0.0	2.464793269168825	0.0
18	1.4316043847179094E-4	0.0	0.0	2.665217883029332	0.0
19	1.4316043847179094E-4	0.0	0.0	2.7505415043585195	0.0
20	1.4316043847179094E-4	0.0	0.0	2.8401599388418606	0.0
21	1.4316043847179094E-4	0.0	0.0	2.9505366369036117	0.0
22	1.4316043847179094E-4	0.0	0.0	3.061199655842306	0.0
23	1.4316043847179094E-4	0.0	0.0	3.1831723494202717	0.0
24	1.4316043847179094E-4	0.0	0.0	3.2750813509191614	0.0
25	1.4316043847179094E-4	0.0	0.0	3.350813222870739	0.0
26	1.4316043847179094E-4	0.0	0.0	3.427547217891619	0.0
27	1.4316043847179094E-4	0.0	0.0	3.502992768966253	0.0
28	1.4316043847179094E-4	0.0	0.0	3.5863121441568353	0.0
29	1.4316043847179094E-4	0.0	0.0	3.674069492940043	0.0
30	1.4316043847179094E-4	0.0	0.0	3.8023412458107675	0.0
31	2.863208769435819E-4	0.0	0.0	3.9062757241412878	0.0
32	2.863208769435819E-4	0.0	0.0	3.9991868487094804	0.0
33	2.863208769435819E-4	0.0	0.0	4.09911283476279	0.0
34	2.863208769435819E-4	0.0	0.0	4.201902029585536	0.0
35	2.863208769435819E-4	0.0	0.0	4.341197136218589	0.0
36	2.863208769435819E-4	0.0	0.0	4.455296005680606	0.0
37	2.863208769435819E-4	0.0	0.0	4.549781895071988	0.0
38	2.863208769435819E-4	0.0	0.0	4.649707881125298	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTACG	65	0.0	44.000004	1
ACAACGA	30	2.5262852E-6	44.0	13
CTATGCG	25	4.4410423E-5	44.0	1
CGGAAAT	20	7.854358E-4	44.0	33
TTGGACG	20	7.854358E-4	44.0	37
CGACGGT	25	4.4410423E-5	44.0	28
GACACGC	25	4.4410423E-5	44.0	35
CGGGGTA	50	2.7284841E-11	44.0	6
AGTGCTA	25	4.4410423E-5	44.0	28
ACGATCT	20	7.854358E-4	44.0	40
TCGGACG	20	7.854358E-4	44.0	1
GTTCGCC	20	7.854358E-4	44.0	34
GTATGCG	30	2.5262852E-6	44.0	1
TATTACG	25	4.4410423E-5	44.0	1
GCTAGCG	35	1.444987E-7	44.0	1
TCGTACA	20	7.854358E-4	44.0	36
CATCGGT	20	7.854358E-4	44.0	31
CCGGTCA	20	7.854358E-4	44.0	31
GGACCGT	20	7.854358E-4	44.0	8
CGCGTAA	25	4.4410423E-5	44.0	31
>>END_MODULE