FastQCFastQC Report
Thu 26 May 2016
SRR1546062_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546062_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences604964
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59120.9772482329527047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGC650.044.0000043
TGATCGG454.783942E-1044.02
AACGTAC207.853522E-444.034
CAACGTA207.853522E-444.033
ATAGGCG800.044.04
CACGGCA207.853522E-444.04
ACGTTAG254.4403307E-544.01
CGTGAAT207.853522E-444.039
GCTATCG207.853522E-444.01
TCGATTG254.4403307E-544.01
TCGATAT207.853522E-444.016
TCGATAG750.044.01
GCTTCGT207.853522E-444.017
GTGCTAG254.4403307E-544.01
CGAGGTA207.853522E-444.036
ACTACGG254.4403307E-544.02
CGAATAA302.5257214E-644.029
CTCGAGG254.4403307E-544.02
GTATACG302.5257214E-644.01
GACGCAT254.4403307E-544.09
Produced by FastQC (version 0.11.5)