##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546062_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	604964
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.038453527813225	28.0	25.0	30.0	16.0	33.0
2	26.014652111530605	30.0	25.0	31.0	10.0	33.0
3	26.348820756276407	30.0	25.0	31.0	16.0	33.0
4	27.800854265708374	32.0	19.0	35.0	10.0	35.0
5	31.151571002572055	33.0	30.0	35.0	19.0	35.0
6	29.78638067719732	33.0	28.0	35.0	17.0	36.0
7	29.83109077564946	32.0	28.0	35.0	17.0	35.0
8	32.41034673137575	33.0	32.0	35.0	28.0	35.0
9	27.082447881196238	31.0	23.0	34.0	10.0	35.0
10	32.04324554849545	35.0	31.0	37.0	18.0	38.0
11	33.85583604974841	35.0	32.0	38.0	27.0	39.0
12	33.20443695823222	35.0	31.0	38.0	25.0	39.0
13	32.80271553348629	35.0	31.0	38.0	22.0	39.0
14	32.54515144702825	35.0	30.0	38.0	21.0	40.0
15	31.759157569706627	34.0	27.0	38.0	18.0	40.0
16	30.533906149787427	33.0	27.0	37.0	17.0	40.0
17	30.527763635522113	32.0	26.0	37.0	17.0	40.0
18	31.477854880620995	34.0	28.0	38.0	18.0	39.0
19	31.032901131306986	34.0	27.0	37.0	18.0	39.0
20	31.72921033317685	34.0	29.0	36.0	20.0	39.0
21	32.51618774009693	34.0	31.0	37.0	23.0	39.0
22	32.85142091099636	34.0	31.0	37.0	25.0	39.0
23	34.13741644130891	35.0	33.0	38.0	28.0	40.0
24	33.810124238797684	35.0	33.0	38.0	27.0	40.0
25	32.59574123418914	35.0	31.0	37.0	23.0	39.0
26	26.19186265629029	33.0	19.0	35.0	0.0	38.0
27	29.429650359360227	33.0	23.0	36.0	12.0	39.0
28	30.986992614436563	34.0	27.0	36.0	18.0	39.0
29	32.3484785871556	34.0	30.0	37.0	23.0	39.0
30	32.05472887642901	34.0	30.0	37.0	22.0	39.0
31	30.889747158508605	33.0	29.0	36.0	18.0	38.0
32	31.035235154488532	34.0	29.0	36.0	18.0	39.0
33	30.77443285881474	34.0	29.0	37.0	17.0	39.0
34	30.757226876309996	34.0	29.0	37.0	15.0	39.0
35	29.972568615653163	34.0	27.0	37.0	10.0	39.0
36	29.648317916438003	33.0	26.0	37.0	10.0	39.0
37	29.61120661725326	33.0	26.0	37.0	10.0	39.0
38	29.359394939202993	33.0	26.0	36.0	10.0	38.0
39	29.23763562790513	33.0	25.0	36.0	9.0	38.0
40	29.163193181743047	33.0	25.0	36.0	10.0	38.0
41	28.628430782658143	33.0	23.0	35.0	9.0	38.0
42	28.866800338532542	33.0	24.0	36.0	9.0	38.0
43	28.419593562592155	33.0	23.0	35.0	8.0	38.0
44	28.165702421962298	32.0	23.0	35.0	8.0	38.0
45	27.61330591572391	31.0	23.0	35.0	8.0	38.0
46	27.219001461243973	31.0	23.0	35.0	8.0	38.0
47	27.373159394608606	31.0	23.0	34.0	8.0	37.0
48	27.557762114770465	31.0	23.0	34.0	8.0	37.0
49	27.3731362527357	31.0	23.0	34.0	8.0	37.0
50	29.14253906017548	34.0	24.0	36.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	10.0
12	36.0
13	102.0
14	215.0
15	473.0
16	985.0
17	1820.0
18	3011.0
19	4765.0
20	6943.0
21	9554.0
22	13117.0
23	17138.0
24	22435.0
25	27349.0
26	31481.0
27	33750.0
28	37436.0
29	44013.0
30	51711.0
31	60747.0
32	64394.0
33	61485.0
34	51184.0
35	37907.0
36	18914.0
37	3889.0
38	92.0
39	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.158131723540574	22.994756712796132	27.351214287131135	25.49589727653216
2	37.26254785408719	18.18571022407945	31.007299607910554	13.544442313922811
3	27.947448112614964	14.396724433189412	44.34263857022897	13.313188883966648
4	25.209433949788746	25.103807829887398	37.141879516797694	12.54487870352616
5	19.129733339504497	28.96899650227121	39.80187250811619	12.099397650108106
6	17.586005117659894	34.57511521346725	37.61347782677977	10.225401842093083
7	76.9391236503329	3.716750087608519	17.86188930250395	1.482236959554618
8	79.69151883417857	2.1290523072447285	16.860176803908995	1.3192520546677158
9	68.01264207456973	2.859839593760951	16.69901018903604	12.42850814263328
10	48.02153516572887	22.866980514543016	21.888244589760713	7.2232397299674025
11	43.49911730284777	16.276671008522822	30.331061021812864	9.893150666816537
12	38.59948691161788	18.63598495117065	32.49085896020259	10.27366917700888
13	18.084712478759066	38.54146693026362	32.32539456893303	11.048426022044287
14	11.987490164704015	38.94314372425466	36.5833338843303	12.48603222671101
15	10.357475816742815	18.760785765764574	55.19204448529169	15.689693932200925
16	11.792767834119056	14.277874385913872	53.451114446479465	20.47824333348761
17	13.343603916927288	15.453977426755971	37.8888661143473	33.313552541969436
18	18.5756507825259	19.495540230493056	43.78491943322248	18.143889553758573
19	25.89740877143103	20.41014010751053	35.28127293524904	18.411178185809405
20	27.692061015200903	19.774234499904125	34.16087568847072	18.37282879642425
21	18.84376591003762	25.173398747694076	37.409994644309414	18.57284069795889
22	19.98780092699731	21.3262276763576	34.04962939943534	24.63634199720975
23	15.47249092507984	27.153847171071337	34.682063726106016	22.691598177742808
24	15.961776237924902	19.7013376002539	49.587413465925245	14.749472695895955
25	13.840658287104688	20.88504439933616	46.289365978802046	18.98493133475711
26	11.139671120926204	42.20432951382231	30.570744705470076	16.08525465978141
27	13.673375605821173	31.970332118936	37.835309208481824	16.520983066760998
28	12.677283276360246	25.206293267037378	47.64911631105322	14.467307145549157
29	12.707202412044353	21.03282178774274	46.25382667398391	20.006149126228998
30	15.893838311039996	26.97036517875444	39.529790202392206	17.606006307813356
31	26.74291362791836	23.203198868031816	33.485132999649565	16.568754504400264
32	27.419482812200396	22.887312302880833	34.15938799664112	15.533816888277649
33	25.890962106836106	23.978451610343757	31.897600518377956	18.23298576444218
34	17.1568886743674	24.717834449653203	34.544369582322254	23.580907293657145
35	18.602759833642992	23.27940174952559	36.26595962734972	21.851878789481688
36	28.385986604161573	21.70013422286285	31.200864844850273	18.71301432812531
37	18.92591955884978	30.760673362381898	32.2437368173974	18.069670261370923
38	18.423740916814886	31.729160743449196	28.873453626992678	20.973644712743237
39	19.63224257972375	29.557626569514877	30.89423502886122	19.915895821900147
40	23.396764104971535	24.31698415112304	28.666499163586597	23.619752580318828
41	16.722152061940875	21.805925641856376	31.262190808048086	30.209731488154667
42	20.015240576298755	23.048974815030316	27.312203701377275	29.623580907293658
43	20.65164208118169	23.828855931923222	29.19793574493689	26.321566241958198
44	17.98272293888562	28.507812035096304	30.594878372927976	22.9145866530901
45	15.911855912087331	36.05437678936268	25.469945319060304	22.56382197948969
46	20.409644210234	29.980461647304637	28.578560046548223	21.031334095913145
47	19.898374118129343	25.344483308097672	29.94260815519601	24.814534418576972
48	20.289637069313216	22.18479116112694	35.01117421863119	22.51439755092865
49	19.43520606184831	22.47257688060777	34.70685858993263	23.385358467611294
50	18.227696193492505	30.868283071389374	30.37073280393544	20.53328793118268
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	131.0
1	303.5
2	476.0
3	5374.5
4	10273.0
5	8467.0
6	6661.0
7	6832.5
8	7004.0
9	7684.5
10	8365.0
11	9053.5
12	9742.0
13	9812.0
14	9882.0
15	9535.5
16	9189.0
17	8930.5
18	8672.0
19	8009.0
20	7346.0
21	6899.5
22	6453.0
23	6109.5
24	5766.0
25	5715.0
26	5664.0
27	5961.5
28	6259.0
29	6679.0
30	7099.0
31	8602.5
32	10106.0
33	11170.5
34	12235.0
35	13688.5
36	15142.0
37	15343.0
38	15544.0
39	20610.0
40	25676.0
41	36877.5
42	48079.0
43	54074.0
44	60069.0
45	59406.0
46	58743.0
47	53920.5
48	49098.0
49	46959.5
50	44821.0
51	42273.5
52	39726.0
53	35308.5
54	30891.0
55	27063.5
56	23236.0
57	20215.0
58	17194.0
59	15413.5
60	13633.0
61	12214.5
62	10796.0
63	9029.0
64	7262.0
65	6165.0
66	5068.0
67	4083.0
68	3098.0
69	2482.5
70	1867.0
71	1673.5
72	1480.0
73	1206.5
74	933.0
75	782.0
76	631.0
77	464.0
78	297.0
79	209.5
80	122.0
81	118.0
82	114.0
83	87.0
84	60.0
85	44.5
86	29.0
87	19.5
88	10.0
89	10.5
90	11.0
91	7.5
92	4.0
93	4.5
94	5.0
95	3.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	604964.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.30925728824629
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.70057737635943	36.250098407675175
2	6.382155327293757	5.528128142466341
3	2.492919036031335	3.2389941579074404
4	1.314180532762853	2.2766473126652396
5	0.8498907258729473	1.8404068056862941
6	0.618361118420009	1.606845646481994
7	0.48443505907790707	1.4686365829136308
8	0.3664008739643647	1.269483977692878
9	0.307276640031939	1.1977130755629979
>10	3.0017683370875337	28.434490806179085
>50	0.41912461792474737	12.251861992993437
>100	0.062493730304507306	3.5699487419067797
>500	0.0	0.0
>1k	0.0	0.0
>5k	4.166248686967154E-4	1.0667443498687328
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5912	0.9772482329527047	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.026117256564026952	0.0
2	0.0	0.0	0.0	0.1125686817727997	0.0
3	0.0	0.0	0.0	0.1451326029317447	0.0
4	0.0	0.0	0.0	0.19670591969108905	0.0
5	0.0	0.0	0.0	0.33489596075138356	0.0
6	0.0	0.0	0.0	0.4372160988091853	0.0
7	0.0	0.0	0.0	0.5053193247862683	0.0
8	0.0	0.0	0.0	0.7132655827454195	0.0
9	0.0	0.0	0.0	0.8298014427304765	0.0
10	0.0	0.0	0.0	1.0240278760389048	0.0
11	0.0	0.0	0.0	1.165523898942747	0.0
12	0.0	0.0	0.0	1.3085076136761857	0.0
13	0.0	0.0	0.0	1.365701099569561	0.0
14	0.0	0.0	0.0	1.3860328879073796	0.0
15	0.0	0.0	0.0	1.4142990326697127	0.0
16	0.0	0.0	0.0	1.471492518563088	0.0
17	0.0	0.0	0.0	1.5652171038276659	0.0
18	0.0	0.0	0.0	1.6868772356702217	0.0
19	0.0	0.0	0.0	1.7417565342731138	0.0
20	0.0	0.0	0.0	1.796305234691651	0.0
21	0.0	0.0	0.0	1.8564741042442194	0.0
22	0.0	0.0	0.0	1.9245773302213025	0.0
23	0.0	0.0	0.0	1.9973089307793521	0.0
24	0.0	0.0	0.0	2.0465680602482132	0.0
25	0.0	0.0	0.0	2.0897111233065107	0.0
26	0.0	0.0	0.0	2.1359948691161788	0.0
27	0.0	0.0	0.0	2.194345448654796	0.0
28	0.0	0.0	0.0	2.255175514576074	0.0
29	0.0	0.0	0.0	2.3191462632487223	0.0
30	0.0	0.0	0.0	2.4110525584993487	0.0
31	0.0	0.0	0.0	2.4892390290992523	0.0
32	0.0	0.0	0.0	2.5647807142243173	0.0
33	0.0	0.0	0.0	2.627924967436079	0.0
34	0.0	0.0	0.0	2.7062767371281597	0.0
35	0.0	0.0	0.0	2.8112416606607997	0.0
36	0.0	0.0	0.0	2.8943871040260247	0.0
37	0.0	0.0	0.0	2.981830323787862	0.0
38	0.0	0.0	0.0	3.0661328607983287	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAAGGC	65	0.0	44.000004	3
TGATCGG	45	4.783942E-10	44.0	2
AACGTAC	20	7.853522E-4	44.0	34
CAACGTA	20	7.853522E-4	44.0	33
ATAGGCG	80	0.0	44.0	4
CACGGCA	20	7.853522E-4	44.0	4
ACGTTAG	25	4.4403307E-5	44.0	1
CGTGAAT	20	7.853522E-4	44.0	39
GCTATCG	20	7.853522E-4	44.0	1
TCGATTG	25	4.4403307E-5	44.0	1
TCGATAT	20	7.853522E-4	44.0	16
TCGATAG	75	0.0	44.0	1
GCTTCGT	20	7.853522E-4	44.0	17
GTGCTAG	25	4.4403307E-5	44.0	1
CGAGGTA	20	7.853522E-4	44.0	36
ACTACGG	25	4.4403307E-5	44.0	2
CGAATAA	30	2.5257214E-6	44.0	29
CTCGAGG	25	4.4403307E-5	44.0	2
GTATACG	30	2.5257214E-6	44.0	1
GACGCAT	25	4.4403307E-5	44.0	9
>>END_MODULE