FastQCFastQC Report
Thu 26 May 2016
SRR1546061_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546061_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1075286
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172531.6045033600363066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA302.527564E-644.018
ATTACGT207.856256E-444.012
ATAGCCG254.4426477E-544.030
ACTTCGT207.856256E-444.029
TTGTACG502.7284841E-1144.01
ACTACGG207.856256E-444.02
AATACGG454.802132E-1044.02
TACGTCA302.527564E-644.022
CGTTTTT115700.043.3534971
TACGGGT800.041.254
CGGTCTA1450.040.96551531
TCGATAG501.3478711E-939.6000021
ATCGTAA1000.039.60000214
GTTTTTT130000.039.5492322
TACGGGA2850.039.3684234
AATCGTA950.039.3684213
TATACGG850.038.823532
GTATGCG404.124213E-738.51
TTGCACG800.038.51
TCATCGA2750.038.416
Produced by FastQC (version 0.11.5)