##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546061_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1075286
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.981951778410583	31.0	28.0	33.0	23.0	33.0
2	28.814921797549676	31.0	28.0	33.0	16.0	33.0
3	28.222745390528658	31.0	27.0	33.0	16.0	34.0
4	32.33462632267136	35.0	32.0	35.0	26.0	37.0
5	33.72172612681649	35.0	33.0	35.0	28.0	37.0
6	33.47015119698387	35.0	33.0	37.0	28.0	37.0
7	34.50512235814472	35.0	35.0	37.0	32.0	37.0
8	34.893320474738815	35.0	35.0	37.0	32.0	37.0
9	36.67850041756333	39.0	37.0	39.0	32.0	39.0
10	36.31243780724384	38.0	35.0	39.0	32.0	39.0
11	36.3271538920808	38.0	35.0	39.0	31.0	39.0
12	35.19328811125598	37.0	34.0	39.0	29.0	39.0
13	35.43677589032127	37.0	35.0	39.0	30.0	39.0
14	36.134583729352	38.0	35.0	40.0	30.0	41.0
15	36.69598413817347	38.0	35.0	40.0	31.0	41.0
16	36.27007047427382	38.0	34.0	40.0	31.0	41.0
17	35.84656454189862	37.0	34.0	40.0	30.0	41.0
18	35.51950736827226	37.0	34.0	39.0	29.0	40.0
19	34.95358723167604	37.0	33.0	39.0	27.0	41.0
20	35.470709188067175	36.0	34.0	39.0	30.0	41.0
21	35.6902833292724	36.0	34.0	39.0	30.0	41.0
22	36.01388653809312	37.0	34.0	40.0	31.0	41.0
23	36.35614245884351	37.0	35.0	40.0	32.0	41.0
24	36.05782461596264	37.0	35.0	40.0	31.0	41.0
25	35.68100021761652	36.0	34.0	40.0	30.0	41.0
26	35.80629432541668	37.0	34.0	40.0	31.0	41.0
27	36.09419354478715	37.0	35.0	40.0	31.0	41.0
28	36.03104569388981	37.0	35.0	40.0	31.0	41.0
29	36.16848726757346	37.0	35.0	40.0	31.0	41.0
30	35.781925924823724	37.0	35.0	40.0	30.0	41.0
31	35.12636731065037	36.0	34.0	40.0	29.0	41.0
32	35.097505221866555	37.0	34.0	40.0	29.0	41.0
33	34.84657942166084	37.0	34.0	40.0	26.0	41.0
34	34.57721201615198	37.0	34.0	40.0	24.0	41.0
35	34.36946356597222	37.0	34.0	40.0	23.0	41.0
36	33.99910535429644	36.0	34.0	40.0	21.0	41.0
37	33.9147250126943	37.0	33.0	40.0	20.0	41.0
38	33.76435943553622	36.0	33.0	40.0	20.0	41.0
39	33.57994617246016	36.0	33.0	40.0	18.0	41.0
40	33.39089879343728	36.0	33.0	40.0	18.0	40.0
41	33.12775019855183	36.0	33.0	40.0	18.0	40.0
42	33.300352650364644	36.0	33.0	40.0	18.0	40.0
43	33.06819023032012	35.0	33.0	39.0	18.0	40.0
44	32.82428953785318	35.0	32.0	39.0	15.0	40.0
45	32.81501200610814	35.0	32.0	39.0	17.0	40.0
46	32.65278167854878	35.0	32.0	39.0	17.0	40.0
47	32.644662908286726	35.0	32.0	39.0	17.0	40.0
48	32.67671949602245	35.0	32.0	39.0	18.0	40.0
49	32.63608937529178	35.0	32.0	39.0	17.0	40.0
50	32.82518697351216	35.0	32.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	2.0
11	4.0
12	9.0
13	24.0
14	48.0
15	112.0
16	231.0
17	520.0
18	1072.0
19	1803.0
20	2807.0
21	4136.0
22	6101.0
23	8795.0
24	12806.0
25	19195.0
26	26272.0
27	30259.0
28	29232.0
29	29194.0
30	32603.0
31	39593.0
32	53392.0
33	81387.0
34	129200.0
35	100077.0
36	107074.0
37	151440.0
38	170960.0
39	36933.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.95518029621887	22.54879167030911	27.706954242871202	20.789073790600828
2	37.612969944740286	19.981381697520472	29.520425263604288	12.885223094134957
3	29.321873436462486	18.987227584103206	38.79079612307795	12.900102856356355
4	26.003314467034816	25.070353375753058	34.627345655016434	14.298986502195696
5	20.402013975816665	30.12045167518223	35.60559702255958	13.871937326441525
6	18.905109896343856	37.9350237983197	32.87869459846032	10.281171706876124
7	78.854276908655	4.39045984045175	14.435322323549268	2.319940927343981
8	80.35834187369686	2.9990160757231097	14.241141426560002	2.401500624020028
9	76.66220893790118	4.759012950973044	15.269611991600375	3.30916611952541
10	43.83829046411839	24.89607416073491	20.80209358254455	10.463541792602154
11	37.06874264149259	20.542813725836663	29.409199040999322	12.979244591671424
12	34.10209004860103	20.86319360616617	31.713981210580254	13.320735134652548
13	20.805720524586015	31.983583902329244	31.60563794190569	15.605057631179054
14	16.364855489609276	33.018192369285934	34.341189227796136	16.275762913308643
15	15.593432816943587	22.29825367390629	46.52213457628947	15.586178932860653
16	18.03110986286439	18.777794930837004	44.73591212012432	18.45518308617428
17	18.145870028996935	19.407487868343864	34.17453589091647	28.272106211742738
18	21.488515613520494	22.23845562947904	37.71173436648482	18.56129439051564
19	25.361159728667538	23.081487157835216	31.774337246090806	19.78301586740644
20	27.309385596018178	21.69357733663416	32.27076331320225	18.72627375414541
21	22.281978933976635	25.312056513336916	34.21061931430336	18.19534523838309
22	22.20888210206401	22.414129822205442	32.18976160760951	23.18722646812104
23	19.140489134983625	26.69289844748281	32.21756816326075	21.949044254272817
24	19.12049445449862	22.00819130910288	41.17211606958521	17.699198166813293
25	18.276625939517483	23.240700613604197	38.239686929802865	20.242986517075458
26	17.027748896572632	28.469821052259586	33.98528391516303	20.517146136004747
27	17.27205599254524	29.53288706446471	34.26493044641147	18.930126496578584
28	16.287666723085763	25.52976603433877	40.14848142726679	18.03408581530867
29	17.258106215462675	22.87782041242981	39.289454154522616	20.574619217584903
30	19.1846634290784	26.92437174853946	35.601784083490344	18.28918073889179
31	25.46699203746724	25.23347276910515	31.110327856960847	18.189207336466765
32	26.275893111228083	24.34701093476526	31.431544723915312	17.945551230091343
33	25.52251215025584	25.610116750334328	29.646996241000068	19.220374858409762
34	19.669743677496037	26.02005419953389	32.238027836315176	22.0721742866549
35	20.238243592867384	25.286946914588306	33.507178555286686	20.967630937257624
36	26.65225809691561	25.49628656934062	28.868598679793095	18.98285665395067
37	20.756617309255397	29.8293663267261	30.213543187579862	19.20047317643864
38	21.17976054742645	29.75199156317482	28.407605046471357	20.66064284292737
39	21.44824725700883	28.489722734230703	28.69924838601079	21.36278162274967
40	23.572798306683058	25.440394462496492	28.76695130411816	22.21985592670229
41	18.57915010518132	24.377886441374667	30.55866067260245	26.484302780841563
42	21.422300671635266	25.190693452718627	27.50077653759093	25.886229338055177
43	21.34873884715322	25.639411282207707	28.38035648190342	24.63149338873565
44	19.92279263377371	27.68593657873347	29.795142873616882	22.596127913875936
45	19.167365705496024	31.72132809317707	26.62091759773679	22.490388603590112
46	21.005388333894423	29.6319304817509	28.685205610414343	20.677475573940328
47	20.52811996064303	26.62091759773679	30.306169707408078	22.54479273421211
48	21.040169778086945	24.795449768712697	32.55468777608934	21.60969267711102
49	21.05039961461416	24.78438294556053	32.297267889659125	21.867949550166188
50	19.25032037988033	29.03329904788122	30.486865819884194	21.229514752354255
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	378.0
1	1001.5
2	1625.0
3	14647.5
4	27670.0
5	19386.5
6	11103.0
7	11057.5
8	11012.0
9	11577.5
10	12143.0
11	11932.5
12	11722.0
13	11117.5
14	10513.0
15	9812.0
16	9111.0
17	8442.5
18	7774.0
19	7222.5
20	6671.0
21	6606.5
22	6542.0
23	7096.5
24	7651.0
25	7265.0
26	6879.0
27	8264.0
28	9649.0
29	11545.0
30	13441.0
31	16514.5
32	19588.0
33	22584.5
34	25581.0
35	27406.0
36	29231.0
37	32256.5
38	35282.0
39	41895.5
40	48509.0
41	60942.5
42	73376.0
43	83079.5
44	92783.0
45	90716.5
46	88650.0
47	85531.0
48	82412.0
49	82184.5
50	81957.0
51	75567.0
52	69177.0
53	62729.0
54	56281.0
55	50843.5
56	45406.0
57	43389.0
58	41372.0
59	38853.5
60	36335.0
61	32300.5
62	28266.0
63	25187.0
64	22108.0
65	18312.0
66	14516.0
67	12513.5
68	10511.0
69	9207.0
70	7903.0
71	6536.0
72	5169.0
73	4201.5
74	3234.0
75	2500.5
76	1767.0
77	1297.5
78	828.0
79	688.5
80	549.0
81	405.0
82	261.0
83	205.5
84	150.0
85	131.0
86	112.0
87	73.0
88	34.0
89	31.5
90	29.0
91	20.0
92	11.0
93	10.0
94	9.0
95	6.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1075286.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.444905364119425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.04626338446062	20.87082501881422
2	7.448548888770815	4.088494387046638
3	3.211189038722585	2.6439233782211695
4	2.1287714083829266	2.336957193796506
5	1.4830713423158424	2.0351376319047927
6	1.1069875578982509	1.82287012594651
7	0.8830225486967846	1.6964129198356757
8	0.6852752054568124	1.5045850529711773
9	0.5744237896508858	1.4188505891280665
>10	5.110437720105675	31.32728016517689
>50	1.126863544169764	21.51327146461038
>100	0.1944632441963578	7.060509988481767
>500	3.4116358630926417E-4	0.06544878435619
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	3.4116358630926417E-4	1.6154332997100804
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17253	1.6045033600363066	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.039617366914476704	0.0
2	0.0	0.0	0.0	0.12870994321510743	0.0
3	0.0	0.0	0.0	0.17781315854572644	0.0
4	0.0	0.0	0.0	0.24830603206960752	0.0
5	9.299851388374814E-5	0.0	0.0	0.4221202545183328	0.0
6	9.299851388374814E-5	0.0	0.0	0.5974224531891981	0.0
7	9.299851388374814E-5	0.0	0.0	0.708555677280277	0.0
8	9.299851388374814E-5	0.0	0.0	0.9791813526819841	0.0
9	1.8599702776749628E-4	0.0	0.0	1.1011954028974618	0.0
10	1.8599702776749628E-4	0.0	0.0	1.3251358243295273	0.0
11	2.789955416512444E-4	0.0	0.0	1.5407993780259392	0.0
12	2.789955416512444E-4	0.0	0.0	1.7249364355157604	0.0
13	2.789955416512444E-4	0.0	0.0	1.7990562510811077	0.0
14	2.789955416512444E-4	0.0	0.0	1.8288157755239072	0.0
15	2.789955416512444E-4	0.0	0.0	1.8743850473269437	0.0
16	2.789955416512444E-4	0.0	0.0	1.9790083754461603	0.0
17	2.789955416512444E-4	0.0	0.0	2.107253326091849	0.0
18	2.789955416512444E-4	0.0	0.0	2.28990240735953	0.0
19	4.649925694187407E-4	0.0	0.0	2.366254187258088	0.0
20	5.579910833024888E-4	0.0	0.0	2.455718757614253	0.0
21	5.579910833024888E-4	0.0	0.0	2.565085009941541	0.0
22	5.579910833024888E-4	0.0	0.0	2.680961158240691	0.0
23	6.509895971862369E-4	0.0	0.0	2.808369122261426	0.0
24	6.509895971862369E-4	0.0	0.0	2.908156527658688	0.0
25	6.509895971862369E-4	0.0	0.0	2.985345294182199	0.0
26	6.509895971862369E-4	0.0	0.0	3.068764961135921	0.0
27	6.509895971862369E-4	0.0	0.0	3.159252515144808	0.0
28	7.439881110699851E-4	0.0	0.0	3.2508560513202998	0.0
29	7.439881110699851E-4	0.0	0.0	3.349713471578724	0.0
30	7.439881110699851E-4	0.0	0.0	3.4760984519467377	0.0
31	7.439881110699851E-4	0.0	0.0	3.583790731024118	0.0
32	7.439881110699851E-4	0.0	0.0	3.683950130476915	0.0
33	7.439881110699851E-4	0.0	0.0	3.7876434734572944	0.0
34	7.439881110699851E-4	0.0	0.0	3.9006366678260482	0.0
35	7.439881110699851E-4	0.0	0.0	4.032415561999319	0.0
36	8.369866249537333E-4	0.0	0.0	4.137968875257373	0.0
37	8.369866249537333E-4	0.0	0.0	4.244452173654265	0.0
38	8.369866249537333E-4	0.0	0.0	4.367954200091883	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAA	30	2.527564E-6	44.0	18
ATTACGT	20	7.856256E-4	44.0	12
ATAGCCG	25	4.4426477E-5	44.0	30
ACTTCGT	20	7.856256E-4	44.0	29
TTGTACG	50	2.7284841E-11	44.0	1
ACTACGG	20	7.856256E-4	44.0	2
AATACGG	45	4.802132E-10	44.0	2
TACGTCA	30	2.527564E-6	44.0	22
CGTTTTT	11570	0.0	43.353497	1
TACGGGT	80	0.0	41.25	4
CGGTCTA	145	0.0	40.965515	31
TCGATAG	50	1.3478711E-9	39.600002	1
ATCGTAA	100	0.0	39.600002	14
GTTTTTT	13000	0.0	39.549232	2
TACGGGA	285	0.0	39.368423	4
AATCGTA	95	0.0	39.36842	13
TATACGG	85	0.0	38.82353	2
GTATGCG	40	4.124213E-7	38.5	1
TTGCACG	80	0.0	38.5	1
TCATCGA	275	0.0	38.4	16
>>END_MODULE