FastQCFastQC Report
Thu 26 May 2016
SRR1546060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546060_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1078066
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177191.6435913942189069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCG408.310963E-944.0000041
TAACGCG207.856263E-444.0000041
TCGATAG207.856263E-444.0000041
ACCTACG207.856263E-444.00000433
CGTAATC207.856263E-444.00000440
CGATAAG351.4459692E-744.010
CGTTTTT113000.043.2407071
CGGTCTA1450.042.48275831
TACGGGA2450.042.2040824
AGTACGG750.041.0666662
TGATTCG650.040.61538715
TTATACG603.6379788E-1240.3333321
TCACGAC1550.039.74193225
TATAGCG452.35068E-839.1111151
CAATTAT11250.039.1111144
GTTTTTT129550.038.9903532
ACGGGAT4200.038.76195
TATACGG1250.038.722
GGCGATA3300.038.6666688
CTATACG404.124213E-738.5000041
Produced by FastQC (version 0.11.5)