##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546060_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1078066
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.9507414202841	31.0	28.0	33.0	23.0	33.0
2	28.784009513332208	31.0	28.0	33.0	16.0	33.0
3	28.209169939502775	31.0	27.0	33.0	16.0	34.0
4	32.3211807069326	35.0	32.0	35.0	26.0	37.0
5	33.75069522645181	35.0	33.0	35.0	30.0	37.0
6	33.556623620446246	35.0	33.0	37.0	28.0	37.0
7	34.56272250493012	35.0	35.0	37.0	32.0	37.0
8	34.927445072936166	35.0	35.0	37.0	32.0	37.0
9	36.78343069904811	39.0	37.0	39.0	32.0	39.0
10	36.30207334244842	38.0	35.0	39.0	32.0	39.0
11	36.367110176927945	38.0	35.0	39.0	32.0	39.0
12	35.12830476056197	37.0	34.0	39.0	29.0	39.0
13	35.43621633554903	37.0	35.0	39.0	30.0	39.0
14	36.140838316021465	38.0	35.0	40.0	30.0	41.0
15	36.725651305207656	38.0	35.0	40.0	31.0	41.0
16	36.29635476863198	38.0	34.0	40.0	31.0	41.0
17	35.89850343114429	37.0	34.0	40.0	30.0	41.0
18	35.59532347741233	37.0	34.0	39.0	29.0	40.0
19	35.026975157365136	37.0	33.0	39.0	27.0	41.0
20	35.45037502342157	36.0	34.0	39.0	30.0	41.0
21	35.59555815692175	36.0	34.0	39.0	30.0	40.0
22	35.98345463079255	37.0	34.0	40.0	31.0	41.0
23	36.37635265373363	37.0	35.0	40.0	32.0	41.0
24	35.94222153374654	36.0	35.0	40.0	31.0	41.0
25	35.60187038641419	36.0	34.0	40.0	30.0	41.0
26	35.777347583543126	37.0	34.0	40.0	31.0	41.0
27	36.120781102455695	37.0	35.0	40.0	31.0	41.0
28	36.0499700389401	37.0	35.0	40.0	31.0	41.0
29	36.1867093480362	37.0	35.0	40.0	31.0	41.0
30	35.82675457717802	37.0	35.0	40.0	31.0	41.0
31	35.16030280149824	36.0	34.0	40.0	29.0	41.0
32	35.16038721191467	37.0	34.0	40.0	29.0	41.0
33	34.929155543352636	37.0	34.0	40.0	27.0	41.0
34	34.60165147588366	37.0	34.0	40.0	24.0	41.0
35	34.41629083933637	37.0	34.0	40.0	23.0	41.0
36	34.02430927234511	36.0	34.0	40.0	21.0	41.0
37	33.94349696586294	37.0	33.0	40.0	21.0	41.0
38	33.82341711917452	36.0	33.0	40.0	20.0	41.0
39	33.602017872746195	36.0	33.0	40.0	18.0	41.0
40	33.386802848805175	36.0	33.0	40.0	18.0	40.0
41	33.1167609404248	35.0	33.0	39.0	18.0	40.0
42	33.301893390571635	36.0	33.0	40.0	18.0	40.0
43	33.09422706958572	35.0	33.0	39.0	18.0	40.0
44	32.84719859452019	35.0	33.0	39.0	16.0	40.0
45	32.81931532948818	35.0	32.0	39.0	17.0	40.0
46	32.63462348316337	35.0	32.0	39.0	17.0	40.0
47	32.63564846679146	35.0	32.0	39.0	18.0	40.0
48	32.66603621670659	35.0	32.0	39.0	18.0	40.0
49	32.6828542964902	35.0	32.0	39.0	18.0	40.0
50	32.896503553585774	35.0	33.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	7.0
12	7.0
13	17.0
14	42.0
15	106.0
16	220.0
17	525.0
18	1008.0
19	1732.0
20	2806.0
21	4101.0
22	5921.0
23	8684.0
24	12561.0
25	18841.0
26	26259.0
27	30140.0
28	29299.0
29	29088.0
30	32268.0
31	39526.0
32	53319.0
33	82099.0
34	129826.0
35	100582.0
36	108775.0
37	155671.0
38	171394.0
39	33240.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.01686909706827	22.400854864173436	27.723812827786055	20.858463210972243
2	37.772641007136855	19.881992382655607	29.47917845475138	12.86618815545616
3	29.352655588804392	19.052915127645246	38.64652071394516	12.947908569605202
4	25.895909897909775	25.11274819908985	34.67756148510388	14.313780417896494
5	20.45171631421453	30.119398997835013	35.55663567907716	13.872249008873297
6	18.93557537293635	37.926434930699976	32.97775831906395	10.16023137729972
7	78.67894915524653	4.445831702326203	14.542894405351806	2.3323247370754663
8	80.2641953275588	3.0279222236857484	14.290961777850336	2.416920670905121
9	76.6414115647836	4.729395046314418	15.367240966694062	3.2619524222079166
10	43.841471672420795	24.574654984017677	20.872562533277183	10.711310810284342
11	37.004042424118744	20.51340084929865	29.444393942485895	13.038162784096707
12	33.96934881537865	20.544011220092276	31.72829863848781	13.758341326041263
13	20.860689419757232	31.976984711511168	31.614298196956405	15.548027671775197
14	16.33823903174759	32.93054414108227	34.43258576005551	16.29863106711463
15	15.478273129845482	22.255130947455907	46.6802589080817	15.586337014616916
16	17.996857335265187	18.709615181259775	44.767110733480145	18.526416749994898
17	18.192949225743135	19.37237608829144	34.1650696710591	28.269605014906325
18	21.46343544829352	22.163392593774407	37.76930169395937	18.60387026397271
19	25.329803555626462	22.93635083566312	31.85695495452041	19.876890654190003
20	27.113553344600422	21.606933156226056	32.3295605278341	18.949952971339417
21	22.1963219320524	25.16200306845778	34.273690108026784	18.367984891463045
22	22.175543983392483	22.4093886645159	32.31852224260852	23.096545109483095
23	19.10012930562693	26.72489439422076	32.27047323633247	21.904503063819842
24	19.08612274202136	21.99141796513386	41.141544209723705	17.78091508312107
25	18.249532032361653	23.140141698189165	38.28828661696037	20.322039652488808
26	17.003689941061122	28.47831208849922	33.98001606580674	20.537981904632925
27	17.317863655842963	29.328445568267618	34.42989575777364	18.923795018115776
28	16.241769984397987	25.405680171714906	40.28241313611597	18.07013670777114
29	17.35877024226717	22.735899286314567	39.32866818914612	20.576662282272142
30	19.20819319039836	26.8256303417416	35.6690592227192	18.297117245140836
31	25.4005784432493	25.13936994581037	31.24836512792352	18.21168648301681
32	26.136340446688795	24.330699604662424	31.545842276817933	17.987117671830852
33	25.353457023966993	25.61197551912406	29.85735567210171	19.177211784807238
34	19.702040505868844	25.91529646607907	32.3474629568134	22.03520007123868
35	20.208503004454272	25.253741422139274	33.583194349882106	20.954561223524347
36	26.665528826621003	25.522648891626304	28.8749482870251	18.936873994727595
37	20.64363406322062	29.87405223798914	30.322447790766056	19.159865908024184
38	21.217068342754526	29.745303163257162	28.322013680052983	20.715614813935325
39	21.379303307960736	28.437683778173135	28.787847868312326	21.395165045553796
40	23.52592512888821	25.396682577875566	28.81753065211221	22.25986164112401
41	18.53634193082798	24.360567905861053	30.675580159285236	26.427510004025727
42	21.427723349034288	25.167846866518378	27.617047564805862	25.78738221964147
43	21.498869271454623	25.682008337151903	28.397148226546427	24.42197416484705
44	20.025675607986894	27.682442447864975	29.796691482710706	22.495190461437424
45	19.21236733187022	31.62431613648886	26.729810605287618	22.433505926353302
46	20.947511562371876	29.62490237146891	28.812057888849107	20.615528177310107
47	20.581671252038372	26.48437108674237	30.323282619060425	22.610675042158828
48	21.05641027543768	24.82723692241477	32.61804008288917	21.498312719258376
49	21.117167223528057	24.83836796633972	32.26240322948688	21.78206158064534
50	19.288522224056784	29.049056365751262	30.533195555745195	21.12922585444676
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	349.0
1	1019.5
2	1690.0
3	14889.5
4	28089.0
5	19643.0
6	11197.0
7	11233.5
8	11270.0
9	11851.0
10	12432.0
11	12026.5
12	11621.0
13	11079.5
14	10538.0
15	9874.0
16	9210.0
17	8621.0
18	8032.0
19	7340.5
20	6649.0
21	6608.5
22	6568.0
23	7111.5
24	7655.0
25	7266.0
26	6877.0
27	8296.0
28	9715.0
29	11424.0
30	13133.0
31	16300.5
32	19468.0
33	22501.5
34	25535.0
35	27360.5
36	29186.0
37	32243.0
38	35300.0
39	41869.0
40	48438.0
41	60729.0
42	73020.0
43	82749.0
44	92478.0
45	90635.0
46	88792.0
47	85855.0
48	82918.0
49	82675.5
50	82433.0
51	75726.0
52	69019.0
53	62593.0
54	56167.0
55	51105.5
56	46044.0
57	43854.0
58	41664.0
59	39110.5
60	36557.0
61	32320.0
62	28083.0
63	25084.5
64	22086.0
65	18237.5
66	14389.0
67	12717.0
68	11045.0
69	9500.5
70	7956.0
71	6621.5
72	5287.0
73	4268.5
74	3250.0
75	2546.5
76	1843.0
77	1365.0
78	887.0
79	725.5
80	564.0
81	424.0
82	284.0
83	221.0
84	158.0
85	133.5
86	109.0
87	73.0
88	37.0
89	32.0
90	27.0
91	18.5
92	10.0
93	6.5
94	3.0
95	2.0
96	1.0
97	2.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1078066.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.47906178280017
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.89594070869515	20.855492437979724
2	7.485167628963124	4.113707674617871
3	3.364907697205356	2.773935195147775
4	2.084486085423656	2.291188877069757
5	1.5023278735210346	2.0641280227253653
6	1.125160081494965	1.855100603696437
7	0.854639429074492	1.643928277148852
8	0.6831214257308346	1.501722869024971
9	0.5452199187812107	1.348391865006196
>10	5.153285248537785	31.58792495162749
>50	1.10782136912345	21.10160325405576
>100	0.19724121663845193	7.136244434793948
>500	3.406584052476187E-4	0.0679606666272101
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	3.406584052476187E-4	1.6586708704786994
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17719	1.6435913942189069	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.032465544781117295	0.0
2	0.0	0.0	0.0	0.12244148317449952	0.0
3	0.0	0.0	0.0	0.164646691389952	0.0
4	0.0	0.0	0.0	0.23792606389590248	0.0
5	0.0	0.0	0.0	0.4084165533464556	0.0
6	0.0	0.0	0.0	0.5813187689807489	0.0
7	0.0	0.0	0.0	0.6911450690402999	0.0
8	0.0	0.0	0.0	0.9591249515335796	0.0
9	9.275869937462085E-5	0.0	0.0	1.0767429823405987	0.0
10	9.275869937462085E-5	0.0	0.0	1.2828528123510063	0.0
11	9.275869937462085E-5	0.0	0.0	1.4918381620420271	0.0
12	9.275869937462085E-5	0.0	0.0	1.6720683149269153	0.0
13	1.855173987492417E-4	0.0	0.0	1.745347687432866	0.0
14	1.855173987492417E-4	0.0	0.0	1.7770711626189861	0.0
15	1.855173987492417E-4	0.0	0.0	1.8219663731163027	0.0
16	2.7827609812386254E-4	0.0	0.0	1.9225168032383917	0.0
17	2.7827609812386254E-4	0.0	0.0	2.045700356007888	0.0
18	2.7827609812386254E-4	0.0	0.0	2.222405678316541	0.0
19	2.7827609812386254E-4	0.0	0.0	2.3056102316555758	0.0
20	3.710347974984834E-4	0.0	0.0	2.395400652650209	0.0
21	4.6379349687310423E-4	0.0	0.0	2.5057835049060078	0.0
22	4.6379349687310423E-4	0.0	0.0	2.626462572792389	0.0
23	4.6379349687310423E-4	0.0	0.0	2.75233612784375	0.0
24	4.6379349687310423E-4	0.0	0.0	2.851587936174594	0.0
25	5.565521962477251E-4	0.0	0.0	2.9333083503236352	0.0
26	5.565521962477251E-4	0.0	0.0	3.0173477319570416	0.0
27	5.565521962477251E-4	0.0	0.0	3.1062105659579284	0.0
28	5.565521962477251E-4	0.0	0.0	3.1923833976769513	0.0
29	5.565521962477251E-4	0.0	0.0	3.290243825517176	0.0
30	5.565521962477251E-4	0.0	0.0	3.413334619587298	0.0
31	5.565521962477251E-4	0.0	0.0	3.5163895345925016	0.0
32	5.565521962477251E-4	0.0	0.0	3.6118382362489867	0.0
33	5.565521962477251E-4	0.0	0.0	3.7183252231310515	0.0
34	5.565521962477251E-4	0.0	0.0	3.8244411752156178	0.0
35	6.49310895622346E-4	0.0	0.0	3.9601471524006877	0.0
36	6.49310895622346E-4	0.0	0.0	4.060975858620901	0.0
37	6.49310895622346E-4	0.0	0.0	4.176089404544805	0.0
38	7.420695949969668E-4	0.0	0.0	4.295006057143069	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCG	40	8.310963E-9	44.000004	1
TAACGCG	20	7.856263E-4	44.000004	1
TCGATAG	20	7.856263E-4	44.000004	1
ACCTACG	20	7.856263E-4	44.000004	33
CGTAATC	20	7.856263E-4	44.000004	40
CGATAAG	35	1.4459692E-7	44.0	10
CGTTTTT	11300	0.0	43.240707	1
CGGTCTA	145	0.0	42.482758	31
TACGGGA	245	0.0	42.204082	4
AGTACGG	75	0.0	41.066666	2
TGATTCG	65	0.0	40.615387	15
TTATACG	60	3.6379788E-12	40.333332	1
TCACGAC	155	0.0	39.741932	25
TATAGCG	45	2.35068E-8	39.111115	1
CAATTAT	1125	0.0	39.11111	44
GTTTTTT	12955	0.0	38.990353	2
ACGGGAT	420	0.0	38.7619	5
TATACGG	125	0.0	38.72	2
GGCGATA	330	0.0	38.666668	8
CTATACG	40	4.124213E-7	38.500004	1
>>END_MODULE