FastQCFastQC Report
Thu 26 May 2016
SRR1546059_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546059_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1063609
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179011.6830432988062343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCG351.445951E-744.01
ATATCGT207.856218E-444.012
GCGATCG207.856218E-444.09
TATAGCG700.044.01
GACGCGT207.856218E-444.016
CGTCCAG207.856218E-444.032
ATACCGG408.310963E-944.02
CGTTTTT111600.043.093191
TACGGGA2350.042.127664
CAATTAT10950.040.7853944
GCTACGA650.040.61538710
CGACCCG3750.040.48000332
CGAGGGA4600.040.1739164
TGGGCGA3700.039.8378376
GATGCCA12450.039.405629
GGATGCC12800.039.3593758
AGGGCGC3050.038.950826
GTTTTTT128300.038.7529222
TACCGGG1650.038.6666643
CGGGATC1900.038.2105266
Produced by FastQC (version 0.11.5)