##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546059_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1063609
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.839908274563303	31.0	28.0	33.0	16.0	33.0
2	28.6786384846311	31.0	28.0	33.0	16.0	33.0
3	28.09016095200398	31.0	26.0	33.0	16.0	34.0
4	32.20613496124986	35.0	32.0	35.0	26.0	37.0
5	33.68725913376062	35.0	33.0	35.0	28.0	37.0
6	33.49065587071941	35.0	33.0	37.0	28.0	37.0
7	34.482256167444994	35.0	35.0	37.0	32.0	37.0
8	34.89367897413429	35.0	35.0	37.0	32.0	37.0
9	36.760650765459864	39.0	37.0	39.0	32.0	39.0
10	36.38142870171276	39.0	35.0	39.0	32.0	39.0
11	36.37995353555677	38.0	35.0	39.0	32.0	39.0
12	35.30103731728483	37.0	34.0	39.0	30.0	39.0
13	35.51232078705615	37.0	35.0	39.0	30.0	39.0
14	36.20909375531798	38.0	35.0	40.0	30.0	41.0
15	36.69927106671719	38.0	35.0	40.0	31.0	41.0
16	36.25074816027318	38.0	34.0	40.0	31.0	41.0
17	35.84972297150551	37.0	34.0	40.0	30.0	41.0
18	35.526575085393226	37.0	34.0	39.0	29.0	40.0
19	34.927857887626	37.0	33.0	39.0	27.0	41.0
20	35.532032918111824	36.0	34.0	39.0	30.0	41.0
21	35.66463333800297	36.0	34.0	39.0	30.0	41.0
22	36.00170739435262	37.0	34.0	40.0	31.0	41.0
23	36.36884607031343	37.0	35.0	40.0	32.0	41.0
24	35.95679615347369	36.0	35.0	40.0	31.0	41.0
25	35.62526924837981	36.0	34.0	40.0	30.0	41.0
26	35.78305091438677	37.0	34.0	40.0	31.0	41.0
27	36.12636974677724	37.0	35.0	40.0	31.0	41.0
28	36.05991957570874	37.0	35.0	40.0	31.0	41.0
29	36.181607150748064	37.0	35.0	40.0	31.0	41.0
30	35.82751462238473	37.0	35.0	40.0	31.0	41.0
31	35.129462048553556	36.0	34.0	40.0	29.0	41.0
32	35.151435348892306	37.0	34.0	40.0	29.0	41.0
33	34.93005512364036	37.0	34.0	40.0	27.0	41.0
34	34.60617482552329	37.0	34.0	40.0	24.0	41.0
35	34.390586202260415	37.0	34.0	40.0	23.0	41.0
36	34.04815961504651	37.0	34.0	40.0	21.0	41.0
37	33.98451874702076	37.0	33.0	40.0	21.0	41.0
38	33.83568961902353	36.0	33.0	40.0	20.0	41.0
39	33.58373612859613	36.0	33.0	40.0	18.0	41.0
40	33.436135835631326	36.0	33.0	40.0	18.0	41.0
41	33.146048030808316	36.0	33.0	40.0	18.0	40.0
42	33.34803861193352	36.0	33.0	40.0	18.0	41.0
43	33.14843330584829	35.0	33.0	40.0	18.0	40.0
44	32.9127696362103	35.0	33.0	39.0	16.0	40.0
45	32.90745189256578	35.0	33.0	39.0	17.0	40.0
46	32.72080717632137	35.0	32.0	39.0	17.0	40.0
47	32.734238803921365	35.0	32.0	39.0	18.0	40.0
48	32.75128548178889	35.0	32.0	39.0	18.0	40.0
49	32.77489660204079	35.0	32.0	39.0	18.0	40.0
50	32.91543038842281	35.0	33.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	7.0
11	8.0
12	12.0
13	21.0
14	45.0
15	96.0
16	201.0
17	461.0
18	994.0
19	1788.0
20	2734.0
21	4117.0
22	6049.0
23	8618.0
24	12584.0
25	18486.0
26	26275.0
27	29504.0
28	28688.0
29	28735.0
30	31710.0
31	39276.0
32	52851.0
33	80730.0
34	127773.0
35	98346.0
36	105463.0
37	150361.0
38	170164.0
39	37511.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.977283945510052	22.43606438080159	27.659506453969456	20.927145219718902
2	37.871153779255344	19.778978929286982	29.4562193437626	12.893647947695063
3	29.319514972137316	19.0406436951925	38.75136445817965	12.888476874490532
4	25.845682012844946	25.18199827192135	34.56411143568736	14.408208279546336
5	20.363310201399198	30.14021129945309	35.6455238720244	13.850954627123313
6	18.921897050513863	37.8803676915107	33.02886681101796	10.16886844695748
7	78.51578916688369	4.480969980509755	14.61232464185617	2.390916210750379
8	80.12239460177565	3.074626107902434	14.369566259781555	2.4334130305403585
9	76.52417382703607	4.8117306265742394	15.408669915354235	3.255425631035465
10	43.77745957395998	24.861485752753126	20.947923532049842	10.413131141237052
11	37.06145773493831	20.578426846707764	29.401594006820176	12.958521411533749
12	33.935590992554594	20.931846195359384	31.798527466390375	13.334035345695646
13	20.818270623885283	32.02257596541586	31.59441110408054	15.564742306618315
14	16.354976311783748	32.98025872289535	34.31759227310036	16.347172692220543
15	15.458688296168987	22.359250438836074	46.595318392379156	15.586742872615783
16	17.958385083240174	18.754072220148572	44.79728922940667	18.490253467204585
17	18.15281743573061	19.348087502080183	34.323233443868936	28.175861618320262
18	21.44660302799243	22.15287760821881	37.84727282300169	18.553246540787075
19	25.193750711022567	23.111782619364824	31.911350881762	19.783115787850612
20	27.24826510493988	21.64959115614855	32.29965146966601	18.802492269245562
21	22.16716857416588	25.214435003840695	34.23993215551956	18.378464266473866
22	22.047011636795098	22.372507190142244	32.45609993898134	23.12438123408132
23	19.123568905490647	26.601880954373268	32.28855716715447	21.985992972981613
24	19.084174729623385	22.04616546117981	41.15102448362133	17.71863532557547
25	18.306163261123213	23.126355643850324	38.28869443564317	20.27878665938329
26	16.947017183946357	28.526742440126025	34.06966281782121	20.45657755810641
27	17.220143868658504	29.353738074800045	34.51738373782094	18.90873431872051
28	16.20332283762172	25.487749727578464	40.32243051722955	17.98649691757027
29	17.212716327146534	22.763252285379306	39.50305046309311	20.520980924381046
30	19.065558866087066	26.842100809602027	35.760979833754696	18.331360490556207
31	25.471766410400814	24.958607909485533	31.219367267482696	18.350258412630957
32	26.19411832731765	24.390730052115018	31.542230274471162	17.872921346096167
33	25.447039278531868	25.495647366654477	29.88297391240578	19.174339442407877
34	19.633342703944777	25.98736941864915	32.31666900148456	22.06261887592151
35	20.18448508803517	25.275077589602944	33.63585678571731	20.904580536644577
36	26.648232574188448	25.486433454399126	28.963651116152644	18.90168285525978
37	20.69754956943764	29.839066799923657	30.368302637529393	19.09508099310931
38	21.221520314326035	29.775791667802736	28.32789117053353	20.674796847337696
39	21.394328178870243	28.546956635380106	28.798552851658833	21.26016233409082
40	23.528477100137362	25.490946391013992	28.871417974086345	22.109158534762305
41	18.656009868288066	24.287402607537167	30.71946551787358	26.33712200630119
42	21.417362959508615	25.199297862278335	27.543110297111063	25.840228881101986
43	21.398747095972297	25.748559856112536	28.412038634498206	24.440654413416958
44	19.921606530219282	27.600932297489024	30.074021562435068	22.40343960985663
45	19.253879950244873	31.64217301658786	26.82686964852686	22.27707738464041
46	20.933162468538722	29.592923715387894	28.965625525921652	20.50828829015174
47	20.512049070664126	26.5846753835291	30.39396996452644	22.50930558128034
48	21.0892348598028	24.839579206268468	32.586599022761185	21.484586911167543
49	21.019472381298016	24.877939167494823	32.447544163315655	21.65504428789151
50	19.216930281710667	29.042533487399975	30.620933068449023	21.119603162440335
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	422.0
1	1052.5
2	1683.0
3	15110.0
4	28537.0
5	19889.5
6	11242.0
7	11231.0
8	11220.0
9	11556.0
10	11892.0
11	11733.0
12	11574.0
13	10975.0
14	10376.0
15	9716.0
16	9056.0
17	8371.0
18	7686.0
19	7100.5
20	6515.0
21	6501.0
22	6487.0
23	6940.5
24	7394.0
25	7204.5
26	7015.0
27	8201.5
28	9388.0
29	11260.5
30	13133.0
31	16133.5
32	19134.0
33	22221.5
34	25309.0
35	27020.5
36	28732.0
37	31903.5
38	35075.0
39	41800.5
40	48526.0
41	60361.5
42	72197.0
43	81754.5
44	91312.0
45	89644.5
46	87977.0
47	84908.0
48	81839.0
49	81665.0
50	81491.0
51	74895.5
52	68300.0
53	61535.0
54	54770.0
55	49899.5
56	45029.0
57	42800.0
58	40571.0
59	38306.0
60	36041.0
61	31823.0
62	27605.0
63	24696.5
64	21788.0
65	17891.5
66	13995.0
67	12191.0
68	10387.0
69	9039.5
70	7692.0
71	6495.0
72	5298.0
73	4243.0
74	3188.0
75	2485.0
76	1782.0
77	1301.5
78	821.0
79	660.0
80	499.0
81	398.0
82	297.0
83	212.0
84	127.0
85	127.5
86	128.0
87	81.0
88	34.0
89	30.0
90	26.0
91	16.0
92	6.0
93	7.0
94	8.0
95	5.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1063609.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.998091623709097
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.48873940458148	20.3805190300319
2	7.387038554849223	3.988718874633819
3	3.440552297146164	2.7866503846354482
4	2.1739154571891386	2.3476627478155923
5	1.5284015997191096	2.0631963213520037
6	1.159630230379533	1.878468192564571
7	0.907681331504641	1.7153964627164797
8	0.7313147232942031	1.5795281524211489
9	0.5655663694190133	1.3742291394776724
>10	5.272432734008662	31.593143676061946
>50	1.1419816562690226	21.37398175972632
>100	0.20204288378320273	7.1495463218698
>500	3.5137892831855745E-4	0.07076974134033304
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	3.5137892831855745E-4	1.698189195352951
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17901	1.6830432988062343	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.033188888021820045	0.0
2	0.0	0.0	0.0	0.12485791301126636	0.0
3	0.0	0.0	0.0	0.17064541574958467	0.0
4	0.0	0.0	0.0	0.2385275039981798	0.0
5	0.0	0.0	0.0	0.4031556709279444	0.0
6	0.0	0.0	0.0	0.560826393909792	0.0
7	0.0	0.0	0.0	0.6672564824103594	0.0
8	0.0	0.0	0.0	0.9224254401758541	0.0
9	0.0	0.0	0.0	1.0412661043673004	0.0
10	0.0	0.0	0.0	1.2574169643167743	0.0
11	9.401951280968852E-5	0.0	0.0	1.4633196973699922	0.0
12	9.401951280968852E-5	0.0	0.0	1.6397003034009678	0.0
13	9.401951280968852E-5	0.0	0.0	1.7149159136487186	0.0
14	9.401951280968852E-5	0.0	0.0	1.7448141187221997	0.0
15	9.401951280968852E-5	0.0	0.0	1.7885331921787049	0.0
16	1.8803902561937704E-4	0.0	0.0	1.8809543732706286	0.0
17	2.8205853842906556E-4	0.0	0.0	2.0008292521029816	0.0
18	2.8205853842906556E-4	0.0	0.0	2.1693122190579435	0.0
19	2.8205853842906556E-4	0.0	0.0	2.24575008297222	0.0
20	2.8205853842906556E-4	0.0	0.0	2.3318719567058945	0.0
21	2.8205853842906556E-4	0.0	0.0	2.4397123378986074	0.0
22	3.760780512387541E-4	0.0	0.0	2.550279284962801	0.0
23	3.760780512387541E-4	0.0	0.0	2.6744790613843996	0.0
24	3.760780512387541E-4	0.0	0.0	2.77094308152714	0.0
25	3.760780512387541E-4	0.0	0.0	2.851047706440995	0.0
26	3.760780512387541E-4	0.0	0.0	2.9335968386879014	0.0
27	3.760780512387541E-4	0.0	0.0	3.024795766113299	0.0
28	3.760780512387541E-4	0.0	0.0	3.1103535227701156	0.0
29	3.760780512387541E-4	0.0	0.0	3.2009883331186555	0.0
30	4.700975640484426E-4	0.0	0.0	3.320957231463818	0.0
31	5.641170768581311E-4	0.0	0.0	3.4198657589396104	0.0
32	5.641170768581311E-4	0.0	0.0	3.512663018082773	0.0
33	5.641170768581311E-4	0.0	0.0	3.607998804071797	0.0
34	5.641170768581311E-4	0.0	0.0	3.715181048674842	0.0
35	5.641170768581311E-4	0.0	0.0	3.836184161660911	0.0
36	5.641170768581311E-4	0.0	0.0	3.9409218989309043	0.0
37	5.641170768581311E-4	0.0	0.0	4.0481981630467585	0.0
38	5.641170768581311E-4	0.0	0.0	4.168261080904731	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCG	35	1.445951E-7	44.0	1
ATATCGT	20	7.856218E-4	44.0	12
GCGATCG	20	7.856218E-4	44.0	9
TATAGCG	70	0.0	44.0	1
GACGCGT	20	7.856218E-4	44.0	16
CGTCCAG	20	7.856218E-4	44.0	32
ATACCGG	40	8.310963E-9	44.0	2
CGTTTTT	11160	0.0	43.09319	1
TACGGGA	235	0.0	42.12766	4
CAATTAT	1095	0.0	40.78539	44
GCTACGA	65	0.0	40.615387	10
CGACCCG	375	0.0	40.480003	32
CGAGGGA	460	0.0	40.173916	4
TGGGCGA	370	0.0	39.837837	6
GATGCCA	1245	0.0	39.40562	9
GGATGCC	1280	0.0	39.359375	8
AGGGCGC	305	0.0	38.95082	6
GTTTTTT	12830	0.0	38.752922	2
TACCGGG	165	0.0	38.666664	3
CGGGATC	190	0.0	38.210526	6
>>END_MODULE