FastQCFastQC Report
Thu 26 May 2016
SRR1546058_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546058_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences930943
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89170.9578459690872587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATCGG302.5271966E-644.02
GCGTGCC207.8557094E-444.08
GCTCGAA302.5271966E-644.021
ACGTTAG650.043.9999961
AGTACGG700.043.9999962
CGTTTTT101600.043.1771661
ATACCGG700.040.857142
TCGATAG557.8216544E-1140.01
ACGACGT557.8216544E-1140.026
CGTTAGG1600.039.8752
GTTGATC2550.039.6862716
CGGCATA501.3478711E-939.66
TTACGAC501.3478711E-939.624
GCGGAAT3750.039.3066678
GTTTTTT116000.038.8982772
CGGGAAT11050.038.8235329
TACCGGG1700.038.8235323
TACGGGA2100.038.7619064
CGACGAA404.1234125E-738.517
CTACGCG404.1234125E-738.51
Produced by FastQC (version 0.11.5)