FastQCFastQC Report
Thu 26 May 2016
SRR1546057_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546057_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences981400
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161951.6501936009781943No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACA207.855918E-444.00000428
CGTTAGA207.855918E-444.00000425
ATCCGAT207.855918E-444.00000426
GTACTCA207.855918E-444.0000049
TACGTAG650.044.0000041
CTCGTAG302.5273366E-644.01
TAGCGAT351.4457873E-744.028
CGTTTTT103000.043.0388371
AACGGGA1100.042.04
CGGTCTA1700.041.41176231
GCGATAT700.040.8571439
TCACGAC1800.040.33333225
CTAACGG603.6379788E-1240.3333322
TATGGGC2200.040.04
CCCTACA2650.039.0188727
TCGTTGA850.038.8235324
TACGGGA1700.038.823534
TGTTACG404.1237217E-738.5000041
TGCGTAG404.1237217E-738.5000041
GTTTTTT120100.038.394672
Produced by FastQC (version 0.11.5)