##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546057_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	981400
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.981059710617487	31.0	28.0	33.0	23.0	33.0
2	28.783039535357652	31.0	28.0	33.0	16.0	33.0
3	28.15349500713267	31.0	27.0	33.0	16.0	34.0
4	32.31517322192786	35.0	32.0	35.0	26.0	37.0
5	33.66771652740982	35.0	33.0	35.0	28.0	37.0
6	33.44131037293662	35.0	33.0	37.0	28.0	37.0
7	34.47358263704911	35.0	35.0	37.0	32.0	37.0
8	34.84295088648869	35.0	35.0	37.0	32.0	37.0
9	36.595318932137765	39.0	37.0	39.0	32.0	39.0
10	36.28783574485429	38.0	35.0	39.0	31.0	39.0
11	36.2876727124516	38.0	35.0	39.0	31.0	39.0
12	35.05720501324638	37.0	34.0	39.0	29.0	39.0
13	35.35789891991033	37.0	35.0	39.0	30.0	39.0
14	35.97539229671897	38.0	34.0	40.0	29.0	41.0
15	36.53622478092521	38.0	35.0	40.0	31.0	41.0
16	36.10508355410638	38.0	34.0	40.0	30.0	41.0
17	35.69651620134502	37.0	34.0	40.0	29.0	41.0
18	35.356022009374364	37.0	33.0	39.0	29.0	40.0
19	34.83905135520685	37.0	33.0	39.0	27.0	40.0
20	35.295872223354394	36.0	34.0	39.0	29.0	40.0
21	35.53996535561443	36.0	34.0	39.0	30.0	40.0
22	35.89503362543306	37.0	34.0	40.0	31.0	41.0
23	36.23622987568779	37.0	35.0	40.0	31.0	41.0
24	35.90396474424292	37.0	35.0	40.0	31.0	41.0
25	35.55498573466476	36.0	34.0	40.0	30.0	41.0
26	35.66110963929081	37.0	34.0	40.0	30.0	41.0
27	35.94076930915019	37.0	35.0	40.0	31.0	41.0
28	35.85333706949256	37.0	35.0	40.0	31.0	41.0
29	35.9763694721826	37.0	35.0	40.0	31.0	41.0
30	35.59019258202568	37.0	35.0	40.0	30.0	41.0
31	34.914655594049314	36.0	34.0	40.0	28.0	41.0
32	34.89597513755859	36.0	34.0	40.0	27.0	41.0
33	34.63831159567964	36.0	34.0	40.0	25.0	41.0
34	34.38249235785612	37.0	34.0	40.0	23.0	41.0
35	34.16459445689831	36.0	34.0	40.0	22.0	41.0
36	33.80783472590177	36.0	33.0	40.0	21.0	41.0
37	33.71616466272672	36.0	33.0	40.0	20.0	41.0
38	33.53946708783371	36.0	33.0	40.0	18.0	41.0
39	33.33288974933768	36.0	33.0	40.0	18.0	41.0
40	33.127278377827594	35.0	33.0	40.0	18.0	40.0
41	32.83003464438557	35.0	32.0	39.0	17.0	40.0
42	32.98487976360302	35.0	33.0	39.0	18.0	40.0
43	32.78434685143672	35.0	32.0	39.0	17.0	40.0
44	32.64843386998166	35.0	32.0	39.0	16.0	40.0
45	32.582245771347054	35.0	32.0	39.0	17.0	40.0
46	32.43302221316487	35.0	32.0	39.0	17.0	40.0
47	32.36778275932342	35.0	32.0	38.0	17.0	40.0
48	32.36420521703688	35.0	32.0	38.0	17.0	40.0
49	32.339932749133894	35.0	32.0	38.0	17.0	40.0
50	32.487393519461996	35.0	32.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	6.0
12	5.0
13	22.0
14	53.0
15	149.0
16	269.0
17	625.0
18	1111.0
19	1832.0
20	2890.0
21	4183.0
22	6124.0
23	8611.0
24	12113.0
25	17858.0
26	24452.0
27	28379.0
28	27597.0
29	27957.0
30	31416.0
31	38412.0
32	51446.0
33	76805.0
34	118300.0
35	93359.0
36	100314.0
37	135312.0
38	142410.0
39	29386.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.66191155492154	22.969125738740576	27.76064805380069	20.608314652537192
2	37.93152639087018	20.06348074179743	29.21662930507438	12.788363562257999
3	29.747911147340535	19.472488282046058	37.74668840432036	13.03291216629305
4	26.296209496637456	25.0179335642959	34.08559201141227	14.600264927654372
5	21.2709394742205	29.219686162624818	35.421133075198696	14.088241287955983
6	19.618402282453637	37.299470144691256	32.865600163032404	10.216527409822703
7	78.80965966985939	4.3385979213368655	14.455981251273691	2.395761157530059
8	80.0951701650703	3.219686162624822	14.225901772977378	2.4592418993274916
9	76.67964132871407	4.464132871408193	15.36937028734461	3.4868555125331158
10	46.41552883635622	21.85500305685755	20.414611779091093	11.314856327695129
11	38.37303851640513	21.273486855512534	26.568677399633177	13.784797228449156
12	33.785204809455884	20.82290605257795	31.153658039535358	14.238231098430813
13	22.207051151416344	29.885673527613616	32.38862848991237	15.518646831057673
14	17.41899327491339	31.352353780313834	35.38098634603627	15.847666598736499
15	16.76849398818015	21.305787650295496	45.48441002649277	16.441308335031586
16	19.88710006113715	17.78031383737518	43.60138577542286	18.731200326064805
17	20.43774200122274	18.021703688608113	33.268493988180154	28.272060321988995
18	22.73863867943754	21.803953535765235	36.63816996127981	18.819237823517422
19	27.233136335846748	22.587731811697576	30.726309353984107	19.452822498471573
20	27.514367230487057	22.24852251885062	30.32830650091706	19.908803749745264
21	23.650091705726513	23.9249031995109	33.17648257591196	19.24852251885062
22	22.87415936417363	21.232932545343385	32.04473201548807	23.848176074994907
23	20.736498879152233	25.32677807214184	31.461279804361116	22.475443244344813
24	21.131037293662118	22.164051355206848	38.10006113715101	18.60485021398003
25	20.57122478092521	22.18208681475443	36.38669248013043	20.859995924189935
26	18.700122274302018	26.228754840024454	32.85897697167312	22.21214591400041
27	19.077644181781128	25.792745058080293	34.998573466476465	20.131037293662114
28	17.728652944772776	24.139698390055024	38.965763195435095	19.16588546973711
29	18.628388017118404	23.37578968820053	37.723252496433666	20.272569798247403
30	21.401161605869166	24.43927042999796	35.36784185856939	18.79172610556348
31	27.58141430609334	22.396372529040146	30.68096596698594	19.34124719788058
32	27.401263501120848	23.244141023028327	30.75922152027715	18.59537395557367
33	26.050438149582227	23.487364988791523	29.435500305685757	21.026696555940493
34	20.80782555532912	25.010902791929894	31.65610352557571	22.525168127165273
35	21.23334012635011	24.481964540452413	32.97218259629101	21.31251273690646
36	28.03596902384349	23.94640309761565	28.232626859588343	19.785001018952517
37	21.8939270429998	28.793152639087015	28.566741389851234	20.746178928061955
38	22.75565518646831	28.9309150193601	26.839311188098634	21.474118606072956
39	21.805583859792133	26.94487466884043	27.589464030976156	23.660077440391277
40	24.28928061952313	23.850010189525168	28.406969635215003	23.453739555736703
41	20.085592011412267	22.95343386998166	29.666394945995517	27.294579172610554
42	22.962706337884654	24.22121459140004	27.709394742205014	25.106684328510294
43	22.017933564295905	24.022009374363154	28.591807621764826	25.368249439576118
44	21.060321988995312	25.7132667617689	30.272162217240677	22.954249031995108
45	20.35785612390463	29.792948848583656	26.816792337477075	23.032402690034644
46	23.045241491746484	26.565416751579374	28.77807214183819	21.611269614835948
47	21.74903199510903	25.733543916853474	29.929488485836558	22.587935602200936
48	22.209496637456695	24.18830242510699	31.56195231302221	22.0402486244141
49	22.219074791114735	23.66191155492154	31.771245159975546	22.34776849398818
50	20.373751783166906	27.433666191155492	30.411554921540656	21.781027104136946
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	425.0
1	1109.0
2	1793.0
3	13667.5
4	25542.0
5	17627.5
6	9713.0
7	9680.5
8	9648.0
9	10045.5
10	10443.0
11	10379.0
12	10315.0
13	9915.0
14	9515.0
15	8919.0
16	8323.0
17	7630.0
18	6937.0
19	6394.5
20	5852.0
21	5644.5
22	5437.0
23	5459.0
24	5481.0
25	5514.5
26	5548.0
27	6158.5
28	6769.0
29	7585.5
30	8402.0
31	10516.5
32	12631.0
33	14874.5
34	17118.0
35	19204.5
36	21291.0
37	23571.0
38	25851.0
39	31424.5
40	36998.0
41	45959.0
42	54920.0
43	63866.5
44	72813.0
45	75030.0
46	77247.0
47	75357.0
48	73467.0
49	75332.5
50	77198.0
51	71509.0
52	65820.0
53	60092.5
54	54365.0
55	52295.5
56	50226.0
57	48475.5
58	46725.0
59	43848.5
60	40972.0
61	38403.5
62	35835.0
63	31701.5
64	27568.0
65	23686.0
66	19804.0
67	16595.5
68	13387.0
69	12174.5
70	10962.0
71	8759.5
72	6557.0
73	5395.5
74	4234.0
75	3374.0
76	2514.0
77	1936.5
78	1359.0
79	1005.5
80	652.0
81	511.0
82	370.0
83	267.0
84	164.0
85	134.0
86	104.0
87	74.5
88	45.0
89	39.0
90	33.0
91	24.0
92	15.0
93	11.5
94	8.0
95	5.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	981400.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.130628157173334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.52636185781405	22.001303633441
2	7.258526284405033	4.22890860320144
3	3.436211190141429	3.00296971348584
4	2.1398747415057118	2.493435815909212
5	1.4934609139566195	2.1752727275871258
6	1.08211676436644	1.8913644651241388
7	0.8644236687358416	1.7626843124942368
8	0.6989595217633221	1.6288903940322428
9	0.5957847988155524	1.5620026892392962
>10	6.024789922982487	38.87945021003536
>50	0.8102419072255467	15.443230235004377
>100	0.06643630937280819	2.8248670903157467
>500	0.0024606040508443855	0.4472765958784171
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	3.515148644063408E-4	1.6583435142515943
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16195	1.6501936009781943	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.026798451192174444	0.0
2	0.0	0.0	0.0	0.08640717342571837	0.0
3	0.0	0.0	0.0	0.12431220705115141	0.0
4	0.0	0.0	0.0	0.1933971876910536	0.0
5	0.0	0.0	0.0	0.3633584674954147	0.0
6	0.0	0.0	0.0	0.5400448339107398	0.0
7	1.0189525168127165E-4	0.0	0.0	0.6584471163643775	0.0
8	1.0189525168127165E-4	0.0	0.0	0.9568983085388221	0.0
9	1.0189525168127165E-4	0.0	0.0	1.0965966985938456	0.0
10	2.037905033625433E-4	0.0	0.0	1.341756674138985	0.0
11	2.037905033625433E-4	0.0	0.0	1.548705930303648	0.0
12	2.037905033625433E-4	0.0	0.0	1.730690849806399	0.0
13	3.0568575504381494E-4	0.0	0.0	1.8080293458324843	0.0
14	3.0568575504381494E-4	0.0	0.0	1.837578968820053	0.0
15	3.0568575504381494E-4	0.0	0.0	1.874770735683717	0.0
16	3.0568575504381494E-4	0.0	0.0	1.9495618504177705	0.0
17	3.0568575504381494E-4	0.0	0.0	2.0509476258406356	0.0
18	3.0568575504381494E-4	0.0	0.0	2.200937436315468	0.0
19	3.0568575504381494E-4	0.0	0.0	2.276034236804565	0.0
20	3.0568575504381494E-4	0.0	0.0	2.364988791522315	0.0
21	3.0568575504381494E-4	0.0	0.0	2.4687181577338495	0.0
22	3.0568575504381494E-4	0.0	0.0	2.5718361524352966	0.0
23	4.075810067250866E-4	0.0	0.0	2.683309557774608	0.0
24	4.075810067250866E-4	0.0	0.0	2.7787854085999593	0.0
25	4.075810067250866E-4	0.0	0.0	2.851131037293662	0.0
26	4.075810067250866E-4	0.0	0.0	2.9195027511717955	0.0
27	4.075810067250866E-4	0.0	0.0	3.0009170572651316	0.0
28	4.075810067250866E-4	0.0	0.0	3.087018544935806	0.0
29	5.094762584063583E-4	0.0	0.0	3.1808640717342573	0.0
30	5.094762584063583E-4	0.0	0.0	3.3242306908498063	0.0
31	5.094762584063583E-4	0.0	0.0	3.4274505808029345	0.0
32	5.094762584063583E-4	0.0	0.0	3.5287344609741185	0.0
33	6.113715100876299E-4	0.0	0.0	3.634196046464235	0.0
34	6.113715100876299E-4	0.0	0.0	3.7446504992867333	0.0
35	6.113715100876299E-4	0.0	0.0	3.884960260851844	0.0
36	6.113715100876299E-4	0.0	0.0	3.9948033421642553	0.0
37	6.113715100876299E-4	0.0	0.0	4.100774403912777	0.0
38	6.113715100876299E-4	0.0	0.0	4.249541471367435	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACA	20	7.855918E-4	44.000004	28
CGTTAGA	20	7.855918E-4	44.000004	25
ATCCGAT	20	7.855918E-4	44.000004	26
GTACTCA	20	7.855918E-4	44.000004	9
TACGTAG	65	0.0	44.000004	1
CTCGTAG	30	2.5273366E-6	44.0	1
TAGCGAT	35	1.4457873E-7	44.0	28
CGTTTTT	10300	0.0	43.038837	1
AACGGGA	110	0.0	42.0	4
CGGTCTA	170	0.0	41.411762	31
GCGATAT	70	0.0	40.857143	9
TCACGAC	180	0.0	40.333332	25
CTAACGG	60	3.6379788E-12	40.333332	2
TATGGGC	220	0.0	40.0	4
CCCTACA	265	0.0	39.01887	27
TCGTTGA	85	0.0	38.82353	24
TACGGGA	170	0.0	38.82353	4
TGTTACG	40	4.1237217E-7	38.500004	1
TGCGTAG	40	4.1237217E-7	38.500004	1
GTTTTTT	12010	0.0	38.39467	2
>>END_MODULE