FastQCFastQC Report
Thu 26 May 2016
SRR1546056_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546056_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences986993
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164001.6616125950234701No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCACG207.85594E-444.01
TCGTTTA351.4458055E-744.038
ACGTTAC207.85594E-444.020
GCGATAT502.7284841E-1144.09
ACGCTAG207.85594E-444.01
CGTTTTT96000.043.1520841
CGGTCTA1650.042.66666431
TGTACGG950.041.684212
TACGGGA1600.041.254
GTACCGG557.8216544E-1140.02
TCTACGG501.3478711E-939.62
TATGGGC2850.039.3684234
TGCGTAG404.123758E-738.51
CGTAAGG1550.038.322582
GTTTTTT113150.038.2642482
ATCTACC750.038.1333315
ACGGGAG3600.037.888895
CTCACGA2000.037.39999824
TAGACGG1000.037.3999982
AGGGTAC3250.036.5538446
Produced by FastQC (version 0.11.5)