FastQCFastQC Report
Thu 26 May 2016
SRR1546055_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546055_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences972141
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164491.6920385005878775No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA207.8558805E-444.00000438
CTAACGG408.310963E-944.0000042
TACGATC207.8558805E-444.00000411
GCGATAT1100.044.0000049
TAGTACG207.8558805E-444.0000041
CTATACG302.527313E-644.026
CGTTCGA254.442331E-544.014
CGTTCAC351.4457692E-744.020
AATACGG351.4457692E-744.02
TACGTTC254.442331E-544.041
CGTTTTT100650.042.6666681
TGTACGG1150.042.086962
CTACGGG1550.041.1612933
ATAACGC650.040.61538311
TCGTTGA603.6379788E-1240.33333224
TAACGGG1550.039.7419363
TACGGGA1500.039.64
ACAACGA950.039.3684213
AACGGGA1900.039.368424
TCACGAC2100.038.76190625
Produced by FastQC (version 0.11.5)