##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546055_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	972141
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.838888597435968	31.0	28.0	31.0	16.0	33.0
2	28.633917302119755	31.0	28.0	33.0	16.0	33.0
3	28.029634590044036	31.0	26.0	33.0	16.0	34.0
4	32.20445593797608	35.0	32.0	35.0	26.0	37.0
5	33.65569089257628	35.0	33.0	35.0	28.0	37.0
6	33.4836654353638	35.0	33.0	37.0	28.0	37.0
7	34.474962994051275	35.0	35.0	37.0	32.0	37.0
8	34.85795270439165	35.0	35.0	37.0	32.0	37.0
9	36.7150454512257	39.0	37.0	39.0	32.0	39.0
10	36.369310624693334	38.0	35.0	39.0	32.0	39.0
11	36.35257334069852	38.0	35.0	39.0	32.0	39.0
12	35.194402869542586	37.0	34.0	39.0	29.0	39.0
13	35.442056244927436	37.0	35.0	39.0	30.0	39.0
14	36.0650666930003	38.0	34.0	40.0	29.0	41.0
15	36.5461255105998	38.0	35.0	40.0	31.0	41.0
16	36.10039695887736	38.0	34.0	40.0	30.0	41.0
17	35.695474216188806	37.0	34.0	40.0	30.0	41.0
18	35.37802849586634	37.0	33.0	39.0	29.0	40.0
19	34.826237140497106	37.0	33.0	39.0	27.0	41.0
20	35.368155442471824	36.0	34.0	39.0	30.0	40.0
21	35.51877351124991	36.0	34.0	39.0	30.0	40.0
22	35.8926832630246	37.0	34.0	40.0	31.0	41.0
23	36.26178918490219	37.0	35.0	40.0	31.0	41.0
24	35.79337976692681	36.0	34.0	40.0	31.0	41.0
25	35.500867672487836	36.0	34.0	40.0	30.0	41.0
26	35.64337992122542	37.0	34.0	40.0	30.0	41.0
27	35.96940361531918	37.0	35.0	40.0	31.0	41.0
28	35.88071586323383	37.0	35.0	40.0	31.0	41.0
29	36.00075709182104	37.0	35.0	40.0	31.0	41.0
30	35.640950232528	37.0	35.0	40.0	30.0	41.0
31	34.93397562699238	36.0	34.0	40.0	28.0	41.0
32	34.963070171919505	36.0	34.0	40.0	27.0	41.0
33	34.74983978661532	37.0	34.0	40.0	25.0	41.0
34	34.427085165629265	37.0	34.0	40.0	23.0	41.0
35	34.19689942096877	37.0	34.0	40.0	22.0	41.0
36	33.87294127086503	36.0	33.0	40.0	21.0	41.0
37	33.804460464068484	36.0	33.0	40.0	20.0	41.0
38	33.63576065611881	36.0	33.0	40.0	19.0	41.0
39	33.363692098162716	36.0	33.0	40.0	18.0	41.0
40	33.18319050425813	35.0	33.0	40.0	18.0	40.0
41	32.85819958215938	35.0	32.0	39.0	17.0	40.0
42	33.056028909386605	35.0	33.0	39.0	18.0	40.0
43	32.85452830402174	35.0	32.0	39.0	17.0	40.0
44	32.71653186111891	35.0	32.0	39.0	17.0	40.0
45	32.67132545587523	35.0	32.0	39.0	18.0	40.0
46	32.502237844098744	35.0	32.0	39.0	18.0	40.0
47	32.4597501802722	35.0	32.0	39.0	18.0	40.0
48	32.44676955297637	35.0	32.0	38.0	18.0	40.0
49	32.49681373381022	35.0	32.0	39.0	18.0	40.0
50	32.585621838807334	35.0	32.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	4.0
12	9.0
13	20.0
14	53.0
15	113.0
16	270.0
17	580.0
18	1022.0
19	1846.0
20	2743.0
21	4088.0
22	5907.0
23	8131.0
24	11912.0
25	17517.0
26	24151.0
27	27818.0
28	27497.0
29	27637.0
30	30711.0
31	37905.0
32	50038.0
33	76560.0
34	116820.0
35	92140.0
36	99458.0
37	134578.0
38	142562.0
39	30047.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.784301865675864	22.813871650305874	27.651647240472315	20.750179243545947
2	38.05240186351568	20.10335949209014	29.066668312518452	12.777570331875726
3	29.65032850172969	19.553542130205393	37.7954432536021	13.00068611446282
4	26.198154382954737	25.11960713517895	33.955362442279466	14.72687603958685
5	21.231488024885277	29.177351845051287	35.421302053920165	14.169858076143276
6	19.60271195227853	37.39046084878634	32.88987914304612	10.116948055889011
7	78.78702780769457	4.384034826223767	14.403260432385837	2.4256769336958324
8	80.10340063838476	3.2371847293756773	14.212958819759686	2.446455812479877
9	76.7733281489002	4.460875531430111	15.3933431467246	3.3724531729450766
10	46.45365229940924	21.85135695336376	20.375645096750368	11.319345650476627
11	38.38249801211964	21.279320592383204	26.515083717279698	13.823097678217461
12	33.77874197261509	20.886270613007785	31.114005067166183	14.220982347210951
13	22.28092426921609	29.840630114355836	32.362486511730296	15.515959104697775
14	17.505176718192114	31.337120849753276	35.28778232787219	15.869920104182416
15	16.758680068014826	21.36264183899249	45.545965040050774	16.33271305294191
16	19.875409019884977	17.738373342961566	43.65642432527792	18.72979331187554
17	20.378319605900792	18.015287905766755	33.32397255130686	28.282419937025594
18	22.815003173407973	21.83747007892888	36.53688096685563	18.810645780807516
19	27.196466356217876	22.588081358568356	30.81106547301266	19.40438681220111
20	27.54086084220293	22.13125462252904	30.339323205172914	19.98856133009512
21	23.667348666500025	23.881000801324088	33.180474848813084	19.271175683362802
22	22.91786890996265	21.275205962921017	32.0821773796188	23.724747747497535
23	20.814778926102285	25.252303935334485	31.450067428490314	22.482849710072923
24	21.13489709826044	22.15151917263031	38.07996988091234	18.633613848196916
25	20.54064173818407	22.179807250182844	36.357483122304274	20.92206788932881
26	18.742240065998654	26.21440716933037	32.86251685712258	22.180835907548392
27	19.059580863269833	25.764163840430555	35.003975760717836	20.172279535581772
28	17.71913745022584	24.227452602040238	38.95113980379389	19.102270143940025
29	18.67393721692635	23.368009373125915	37.62952082053941	20.328532589408326
30	21.378174565212248	24.331141264487353	35.45267610356934	18.83800806673106
31	27.519156171789895	22.35725064573966	30.770536372810113	19.353056809660327
32	27.304989708283056	23.31338766701538	30.881117039606394	18.500505585095166
33	26.012687459946655	23.42726003738141	29.52421510871365	21.035837393958285
34	20.871560812680464	24.94072361931037	31.826864621490092	22.360850946519076
35	21.382597791884102	24.45694606029372	33.00323718472938	21.157218963092802
36	28.191692357384373	23.86629100099677	28.24250803124238	19.69950861037648
37	21.876764790292764	28.793765513438895	28.51911399683791	20.81035569943043
38	22.75647256930836	28.924816461809556	26.86667880482358	21.452032164058508
39	21.837572944665435	26.956583458572368	27.68250696143872	23.523336635323478
40	24.219531940325528	23.841603224223647	28.453279925442914	23.485584910007912
41	20.11745209799813	23.039147613360615	29.722643114527624	27.12075717411363
42	22.945642658832412	24.31962030199323	27.700097002389573	25.03464003678479
43	22.128374381905505	24.040031230037616	28.61765937245729	25.21393501559959
44	21.106300423498237	25.81436231986924	30.395487897331762	22.68384935930076
45	20.311251145667143	29.915104907621426	26.84466553720088	22.928978409510556
46	23.03842755320473	26.5756716361104	28.950327164475116	21.435573646209757
47	21.915956635920097	25.672201871950673	30.041938360793342	22.369903131335885
48	22.408992111226663	24.18826075641291	31.49841432467101	21.90433280768942
49	22.343672368514444	23.66950884696767	31.805365682550164	22.18145310196772
50	20.41751145152812	27.520081963418885	30.45360703848516	21.608799546567834
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	432.0
1	1096.0
2	1760.0
3	13950.0
4	26140.0
5	17937.0
6	9734.0
7	9725.0
8	9716.0
9	10027.0
10	10338.0
11	10262.5
12	10187.0
13	9695.5
14	9204.0
15	8564.5
16	7925.0
17	7242.0
18	6559.0
19	6228.0
20	5897.0
21	5615.5
22	5334.0
23	5354.5
24	5375.0
25	5396.0
26	5417.0
27	5911.5
28	6406.0
29	7293.5
30	8181.0
31	10285.0
32	12389.0
33	14827.5
34	17266.0
35	19297.0
36	21328.0
37	23539.5
38	25751.0
39	31260.5
40	36770.0
41	45775.5
42	54781.0
43	63348.5
44	71916.0
45	74399.5
46	76883.0
47	74822.5
48	72762.0
49	74353.5
50	75945.0
51	70398.0
52	64851.0
53	59387.0
54	53923.0
55	51692.0
56	49461.0
57	47766.0
58	46071.0
59	43303.5
60	40536.0
61	38144.0
62	35752.0
63	31469.0
64	27186.0
65	23367.0
66	19548.0
67	16644.5
68	13741.0
69	12142.0
70	10543.0
71	8517.0
72	6491.0
73	5405.0
74	4319.0
75	3426.5
76	2534.0
77	1967.0
78	1400.0
79	1052.5
80	705.0
81	534.5
82	364.0
83	253.0
84	142.0
85	113.5
86	85.0
87	64.0
88	43.0
89	33.5
90	24.0
91	19.5
92	15.0
93	11.0
94	7.0
95	4.0
96	1.0
97	2.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	972141.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.544025610725278
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.00502220637158	21.409452747916884
2	7.373624820693416	4.2094587145150495
3	3.4644722127988845	2.9666995070933226
4	2.1393738463762797	2.4426536744752156
5	1.5349011798009644	2.1906129293085583
6	1.161698772758888	1.9895735712946698
7	0.8918119808138447	1.7819132814211418
8	0.7096565233346745	1.6205163181506568
9	0.5952246181200567	1.5291096069377765
>10	6.2183873776546505	39.301169502586816
>50	0.834916728246213	15.581962569273545
>100	0.06800061577796854	2.8149853985350295
>500	0.0025454775959666102	0.4545288001542941
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	3.636396565666586E-4	1.7073633783370594
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16449	1.6920385005878775	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.025304971192450478	0.0
2	0.0	0.0	0.0	0.08434990397483492	0.0
3	0.0	0.0	0.0	0.12066150897863581	0.0
4	0.0	0.0	0.0	0.183923936959762	0.0
5	0.0	0.0	0.0	0.34614320350648725	0.0
6	0.0	0.0	0.0	0.5095968588918686	0.0
7	0.0	0.0	0.0	0.6247036180965518	0.0
8	0.0	0.0	0.0	0.9099503055626704	0.0
9	0.0	0.0	0.0	1.0433671658740862	0.0
10	0.0	0.0	0.0	1.2678202030363908	0.0
11	0.0	0.0	0.0	1.4700542411028852	0.0
12	0.0	0.0	0.0	1.649966414337015	0.0
13	0.0	0.0	0.0	1.7200179809307499	0.0
14	1.0286573655467675E-4	0.0	0.0	1.7460430122790829	0.0
15	1.0286573655467675E-4	0.0	0.0	1.7826632144925478	0.0
16	1.0286573655467675E-4	0.0	0.0	1.859298188225782	0.0
17	1.0286573655467675E-4	0.0	0.0	1.9561977120602876	0.0
18	1.0286573655467675E-4	0.0	0.0	2.100826937656163	0.0
19	3.085972096640302E-4	0.0	0.0	2.1649122915297268	0.0
20	3.085972096640302E-4	0.0	0.0	2.246896283563804	0.0
21	3.085972096640302E-4	0.0	0.0	2.341018432511333	0.0
22	3.085972096640302E-4	0.0	0.0	2.4357577758781903	0.0
23	3.085972096640302E-4	0.0	0.0	2.5410922901101793	0.0
24	3.085972096640302E-4	0.0	0.0	2.6212246988862726	0.0
25	3.085972096640302E-4	0.0	0.0	2.696522418044296	0.0
26	3.085972096640302E-4	0.0	0.0	2.766265387428367	0.0
27	4.11462946218707E-4	0.0	0.0	2.841871703796054	0.0
28	4.11462946218707E-4	0.0	0.0	2.921901246835593	0.0
29	4.11462946218707E-4	0.0	0.0	3.0149947384175753	0.0
30	4.11462946218707E-4	0.0	0.0	3.14707434415378	0.0
31	4.11462946218707E-4	0.0	0.0	3.2510716038105585	0.0
32	5.143286827733837E-4	0.0	0.0	3.352702951526579	0.0
33	5.143286827733837E-4	0.0	0.0	3.4555686880812555	0.0
34	5.143286827733837E-4	0.0	0.0	3.569749655656947	0.0
35	5.143286827733837E-4	0.0	0.0	3.704915233489792	0.0
36	6.171944193280604E-4	0.0	0.0	3.8079867015175783	0.0
37	6.171944193280604E-4	0.0	0.0	3.9105438408625908	0.0
38	6.171944193280604E-4	0.0	0.0	4.045195090012663	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	20	7.8558805E-4	44.000004	38
CTAACGG	40	8.310963E-9	44.000004	2
TACGATC	20	7.8558805E-4	44.000004	11
GCGATAT	110	0.0	44.000004	9
TAGTACG	20	7.8558805E-4	44.000004	1
CTATACG	30	2.527313E-6	44.0	26
CGTTCGA	25	4.442331E-5	44.0	14
CGTTCAC	35	1.4457692E-7	44.0	20
AATACGG	35	1.4457692E-7	44.0	2
TACGTTC	25	4.442331E-5	44.0	41
CGTTTTT	10065	0.0	42.666668	1
TGTACGG	115	0.0	42.08696	2
CTACGGG	155	0.0	41.161293	3
ATAACGC	65	0.0	40.615383	11
TCGTTGA	60	3.6379788E-12	40.333332	24
TAACGGG	155	0.0	39.741936	3
TACGGGA	150	0.0	39.6	4
ACAACGA	95	0.0	39.36842	13
AACGGGA	190	0.0	39.36842	4
TCACGAC	210	0.0	38.761906	25
>>END_MODULE