FastQCFastQC Report
Thu 26 May 2016
SRR1546054_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546054_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences832977
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83521.0026687411537174No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAT254.4417804E-544.031
TGTACGG1100.044.02
CGTTTTT81150.043.186691
CGTAAGG900.041.5555572
CCGTAGG750.041.0666662
ATGCGAG603.6379788E-1240.3333361
ACGATTG557.8216544E-1140.01
TGATTCG557.8216544E-1140.015
AATGCGG501.3478711E-939.6000022
AAATGCG501.3478711E-939.6000021
TGCACGG2150.038.883722
CGGGAAT9350.038.5882349
ACGCTAG404.1227213E-738.5000041
CGGTCTA1200.038.50000431
CCGCAAT404.1227213E-738.50000433
GTTTTTT95450.038.1917232
CGGAATT3650.037.9726039
CGTTTCG357.287792E-637.71428742
TGTAACG357.287792E-637.7142871
AACTTAA13050.037.4252944
Produced by FastQC (version 0.11.5)