##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546054_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 832977 Sequences flagged as poor quality 0 Sequence length 50 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.616934201064375 28.0 23.0 30.0 16.0 33.0 2 25.94324092982159 30.0 25.0 31.0 10.0 33.0 3 26.165799295778875 30.0 25.0 31.0 16.0 33.0 4 28.409519110371594 32.0 19.0 35.0 16.0 37.0 5 31.163580747127472 33.0 30.0 35.0 19.0 37.0 6 29.622406140865834 33.0 28.0 35.0 17.0 37.0 7 29.523482641177367 32.0 28.0 35.0 17.0 35.0 8 32.32277481851239 33.0 32.0 35.0 28.0 35.0 9 26.841705113106364 30.0 23.0 34.0 10.0 35.0 10 31.281708858707983 34.0 29.0 37.0 17.0 37.0 11 33.48921999046792 35.0 32.0 37.0 27.0 39.0 12 32.94985455780892 35.0 31.0 37.0 23.0 39.0 13 33.222034942141256 35.0 32.0 37.0 24.0 39.0 14 32.87841200897504 35.0 31.0 38.0 21.0 40.0 15 31.7187161230142 34.0 27.0 38.0 18.0 40.0 16 30.223405928374973 33.0 25.0 38.0 16.0 40.0 17 30.35492816728433 32.0 25.0 37.0 17.0 40.0 18 31.34723767883147 34.0 27.0 38.0 17.0 39.0 19 31.06200771449872 34.0 27.0 37.0 18.0 39.0 20 31.636399324351093 34.0 29.0 37.0 19.0 39.0 21 32.68315331635807 34.0 31.0 37.0 23.0 39.0 22 33.15432959133325 35.0 31.0 37.0 25.0 39.0 23 34.44603152307927 35.0 33.0 38.0 28.0 40.0 24 34.06056109592462 35.0 33.0 38.0 27.0 40.0 25 32.833678480918444 35.0 31.0 38.0 23.0 40.0 26 26.592216831917327 33.0 21.0 36.0 0.0 39.0 27 29.811068012682224 34.0 24.0 37.0 15.0 39.0 28 31.2667636681445 34.0 28.0 37.0 20.0 39.0 29 32.569010909064716 35.0 30.0 37.0 23.0 39.0 30 32.3487095081857 35.0 30.0 38.0 22.0 39.0 31 31.185749426454752 34.0 29.0 37.0 19.0 39.0 32 31.542749679763066 34.0 30.0 37.0 19.0 39.0 33 31.260309708431326 34.0 29.0 37.0 18.0 39.0 34 31.34236839672644 34.0 30.0 37.0 18.0 39.0 35 30.58505456933385 34.0 29.0 37.0 14.0 39.0 36 30.42678729424702 34.0 28.0 37.0 12.0 39.0 37 30.57732086240076 34.0 28.0 37.0 12.0 39.0 38 30.297239899781147 34.0 28.0 37.0 12.0 39.0 39 30.198039081511254 34.0 27.0 37.0 10.0 39.0 40 30.03374282843344 33.0 27.0 37.0 11.0 39.0 41 29.414969440932943 33.0 26.0 36.0 10.0 38.0 42 29.5795982362058 33.0 26.0 36.0 10.0 38.0 43 29.127361259674636 33.0 26.0 36.0 10.0 38.0 44 28.937708964353156 33.0 25.0 35.0 10.0 38.0 45 28.404120401883844 32.0 24.0 35.0 10.0 38.0 46 28.00990183402423 31.0 23.0 35.0 9.0 38.0 47 28.044033628779665 31.0 23.0 35.0 10.0 38.0 48 28.097132333785925 31.0 23.0 35.0 10.0 37.0 49 27.894600931358248 31.0 24.0 35.0 9.0 37.0 50 29.789958186120383 34.0 27.0 37.0 10.0 39.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 9.0 11 27.0 12 61.0 13 123.0 14 288.0 15 630.0 16 1233.0 17 2228.0 18 3679.0 19 5871.0 20 8449.0 21 11835.0 22 15947.0 23 20838.0 24 26717.0 25 33465.0 26 39863.0 27 44520.0 28 50752.0 29 59919.0 30 71415.0 31 83254.0 32 90931.0 33 90452.0 34 78697.0 35 58238.0 36 27985.0 37 5390.0 38 160.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.88213480084084 23.910864285568508 27.369063011343652 21.837937902247003 2 37.60668061663167 17.362544223910145 30.94119045303772 14.089584706420466 3 31.163645574847802 15.19069554141351 39.10288039165547 14.542778492083213 4 27.421405392946024 25.07536222488736 32.77389411712448 14.729338265042133 5 20.66467621554977 29.457235914076858 35.92091978530019 13.957168085073176 6 19.611826016804788 35.87470002172929 33.9437943664711 10.56967959499482 7 80.90355435984426 3.1096897033171382 14.243610567878825 1.7431453689597673 8 82.76747137075814 1.971603057467373 13.703619667769939 1.5573059040045523 9 70.36064621232039 2.836692969913935 13.821390026375276 12.981270791390397 10 47.28221787636394 21.735534114387313 20.356984646634903 10.625263362613854 11 39.28679903526748 20.952799417030725 26.56724015188895 13.193161395812849 12 34.3661349593086 20.84439306247351 31.323073746333936 13.466398231883952 13 21.712844412270687 30.694484961769653 32.528389139195916 15.06428148676374 14 16.851245592615403 31.707358066309155 35.79714685999733 15.64424948107811 15 15.32059108474784 19.892025830245014 45.448913955607416 19.338469129399734 16 17.910698614727657 16.009685741623116 43.70900997266431 22.370605670984915 17 19.6018617560869 17.183787787658 33.922905434363734 29.29144502189136 18 22.260158443750548 21.545612904077785 37.56154131506632 18.632687337105345 19 27.749385637298506 22.054390457359567 31.33772000907588 18.858503896266043 20 27.99453046122522 21.72893129102004 30.625455444748173 19.651082803006563 21 23.50244964746926 23.76620242815828 33.48988027280465 19.24146765156781 22 22.62607490963136 20.945716388327646 32.118653936423215 24.309554765617776 23 20.304762316366478 25.411265857280576 31.802078568796016 22.48189325755693 24 20.38447640210954 21.681631065443582 39.73098897088395 18.202903561562923 25 20.14161255352789 21.016786778026283 37.89324315077127 20.948357517674555 26 15.033548345272438 38.530715734047874 27.371343986688707 19.064391933990972 27 18.35344793433672 26.678167584459118 35.326905784913635 19.641478696290534 28 17.03852567357802 24.154208339485965 40.21623646271146 18.59102952422456 29 18.009861016570685 23.184793817836507 38.59758432705825 20.207760838534558 30 20.884010002677144 24.411358296807713 35.9526133374631 18.75201836305204 31 28.330794247620283 22.09772898891566 30.87528227069895 18.696194492765105 32 27.666310114204833 22.767255278357027 31.110943039243583 18.45549156819456 33 26.23037610882413 23.109641682783558 29.737915932852886 20.922066275539418 34 20.548706626953685 24.51544280334271 31.782750304030007 23.1531002656736 35 21.135997752639028 23.951561687777694 33.2346511368261 21.677789422757172 36 28.47533605369656 23.454669216557 28.046512688825743 20.023482040920698 37 21.28450125273567 29.319056828699953 28.133189751937927 21.26325216662645 38 22.23350704761356 29.6662452864845 26.66160050037396 21.43864716552798 39 21.46829984501373 27.06089123709298 27.421285341612073 24.049523576281217 40 24.333204878406008 23.274832318299303 28.376653857189332 24.015308946105353 41 19.512423512293857 22.50830455102602 29.7407971648677 28.238474771812427 42 22.564488575314805 23.796455364313783 26.791375992374338 26.847680067997075 43 21.621605398468386 23.443264339831714 28.646049050574025 26.289081211125875 44 20.582080897791897 25.407184111926263 30.418126791015837 23.592608199266007 45 19.739200482126158 30.75943273343682 26.355109444798597 23.146257339638428 46 22.38261080437995 26.572522410582767 28.392740735938688 22.652126049098595 47 21.357852617779365 25.28028985194069 29.433225647286783 23.928631882993166 48 21.778872645943405 23.74435308537931 31.997882294469115 22.47889197420817 49 21.80732481208965 23.713739995221957 31.558974617546465 22.919960575141932 50 20.06694062381074 28.030425810076387 30.24681353746862 21.65582002864425 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 260.0 1 651.0 2 1042.0 3 7965.0 4 14888.0 5 11712.0 6 8536.0 7 8267.5 8 7999.0 9 8526.5 10 9054.0 11 9315.0 12 9576.0 13 9439.5 14 9303.0 15 9015.5 16 8728.0 17 8236.0 18 7744.0 19 7243.0 20 6742.0 21 6438.5 22 6135.0 23 6068.5 24 6002.0 25 5889.5 26 5777.0 27 5965.0 28 6153.0 29 7115.0 30 8077.0 31 9686.0 32 11295.0 33 13058.5 34 14822.0 35 16297.5 36 17773.0 37 19764.0 38 21755.0 39 25639.0 40 29523.0 41 37092.0 42 44661.0 43 52055.5 44 59450.0 45 62099.0 46 64748.0 47 63460.5 48 62173.0 49 62326.5 50 62480.0 51 58786.0 52 55092.0 53 50890.5 54 46689.0 55 44888.0 56 43087.0 57 41359.5 58 39632.0 59 37615.5 60 35599.0 61 33174.0 62 30749.0 63 27321.0 64 23893.0 65 20632.5 66 17372.0 67 14785.5 68 12199.0 69 10718.5 70 9238.0 71 7543.0 72 5848.0 73 4832.5 74 3817.0 75 3068.0 76 2319.0 77 1837.0 78 1355.0 79 1017.0 80 679.0 81 530.0 82 381.0 83 260.0 84 139.0 85 120.5 86 102.0 87 76.5 88 51.0 89 34.5 90 18.0 91 13.0 92 8.0 93 9.0 94 10.0 95 5.5 96 1.0 97 1.5 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 832977.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.35576474130285 #Duplication Level Percentage of deduplicated Percentage of total 1 81.11490490651072 36.790285439528525 2 7.2996310004366 6.621606927082471 3 2.9222616019619942 3.9762422919339304 4 1.645906382793177 2.986053707367044 5 1.0771768025834718 2.4428088821382388 6 0.813787676977216 2.2145977455809978 7 0.5703990107093216 1.8109618336882713 8 0.49731594571693827 1.8044916028828795 9 0.4153050993262576 1.6952832342814634 >10 3.434217818817628 31.73016086558651 >50 0.19034743891459371 5.485579520351864 >100 0.01845791040204679 1.3494176520179002 >500 0.0 0.0 >1k 0.0 0.0 >5k 2.884048500309767E-4 1.0925102975599168 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8352 1.0026687411537174 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.017287392088857195 0.0 2 0.0 0.0 0.0 0.06086602631285137 0.0 3 0.0 0.0 0.0 0.09027860313069869 0.0 4 0.0 0.0 0.0 0.13589811003184962 0.0 5 0.0 0.0 0.0 0.2512674419581813 0.0 6 0.0 0.0 0.0 0.3505498951351598 0.0 7 0.0 0.0 0.0 0.42558197885415805 0.0 8 0.0 0.0 0.0 0.6039782611044483 0.0 9 0.0 0.0 0.0 0.7043411762869803 0.0 10 0.0 0.0 0.0 0.8564462164021336 0.0 11 0.0 0.0 0.0 0.9937849424413879 0.0 12 0.0 0.0 0.0 1.1111951470448764 0.0 13 0.0 0.0 0.0 1.1562143972762753 0.0 14 0.0 0.0 0.0 1.1766231240478429 0.0 15 0.0 0.0 0.0 1.19991308283422 0.0 16 0.0 0.0 0.0 1.247093257076726 0.0 17 0.0 0.0 0.0 1.3173232874377083 0.0 18 0.0 0.0 0.0 1.4125239952603734 0.0 19 0.0 0.0 0.0 1.4497399087849965 0.0 20 0.0 0.0 0.0 1.5054437277379809 0.0 21 0.0 0.0 0.0 1.5597069306835603 0.0 22 0.0 0.0 0.0 1.6169714169778997 0.0 23 0.0 0.0 0.0 1.6791580079642054 0.0 24 0.0 0.0 0.0 1.728619157551769 0.0 25 0.0 0.0 0.0 1.770036867764656 0.0 26 0.0 0.0 0.0 1.8088134486306344 0.0 27 0.0 0.0 0.0 1.8659578835910235 0.0 28 0.0 0.0 0.0 1.9231023185514127 0.0 29 0.0 0.0 0.0 1.9910513735673374 0.0 30 0.0 0.0 0.0 2.0933351100930757 0.0 31 0.0 0.0 0.0 2.176890838522552 0.0 32 0.0 0.0 0.0 2.2465206122137826 0.0 33 0.0 0.0 0.0 2.3246740306154914 0.0 34 0.0 0.0 0.0 2.402347243681398 0.0 35 0.0 0.0 0.0 2.5036705695355335 0.0 36 0.0 0.0 0.0 2.5841049632822997 0.0 37 0.0 0.0 0.0 2.6668203323741233 0.0 38 0.0 0.0 0.0 2.7667030422208536 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTAT 25 4.4417804E-5 44.0 31 TGTACGG 110 0.0 44.0 2 CGTTTTT 8115 0.0 43.18669 1 CGTAAGG 90 0.0 41.555557 2 CCGTAGG 75 0.0 41.066666 2 ATGCGAG 60 3.6379788E-12 40.333336 1 ACGATTG 55 7.8216544E-11 40.0 1 TGATTCG 55 7.8216544E-11 40.0 15 AATGCGG 50 1.3478711E-9 39.600002 2 AAATGCG 50 1.3478711E-9 39.600002 1 TGCACGG 215 0.0 38.88372 2 CGGGAAT 935 0.0 38.588234 9 ACGCTAG 40 4.1227213E-7 38.500004 1 CGGTCTA 120 0.0 38.500004 31 CCGCAAT 40 4.1227213E-7 38.500004 33 GTTTTTT 9545 0.0 38.191723 2 CGGAATT 365 0.0 37.972603 9 CGTTTCG 35 7.287792E-6 37.714287 42 TGTAACG 35 7.287792E-6 37.714287 1 AACTTAA 1305 0.0 37.42529 44 >>END_MODULE