FastQCFastQC Report
Thu 26 May 2016
SRR1546053_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546053_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences390663
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70071.7936175168879571No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCC6400.16382406319513237No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT5090.13029132525987872No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA4360.11160514305168392No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGG207.8500924E-444.0000042
ATTTACG207.8500924E-444.0000041
CGGAAAT207.8500924E-444.00000439
TCGTTGA800.044.00000424
GACCGTC207.8500924E-444.00000410
CGTTGAT800.044.00000425
CGATTCT207.8500924E-444.00000410
CCGTCGG207.8500924E-444.00000412
ATCGTAG207.8500924E-444.0000041
CACTCGC207.8500924E-444.00000410
GCTTAAG207.8500924E-444.0000041
CGGGATA207.8500924E-444.0000046
ACTCGCT207.8500924E-444.00000411
CTACAAC207.8500924E-444.00000440
AACTCGT207.8500924E-444.00000444
GACGAGG408.289135E-944.0000042
AGAATGC207.8500924E-444.00000421
TATCCAG207.8500924E-444.0000041
AGGTATG302.5234112E-644.01
GTCGATG351.4428042E-744.01
Produced by FastQC (version 0.11.5)