##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546053_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	390663
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.121943977289888	31.0	28.0	33.0	25.0	33.0
2	28.8022182802057	31.0	28.0	33.0	16.0	33.0
3	28.269969257390642	31.0	27.0	33.0	16.0	34.0
4	32.14405254656827	35.0	32.0	35.0	25.0	37.0
5	33.62641714214041	35.0	33.0	35.0	28.0	37.0
6	33.44206131627515	35.0	33.0	37.0	28.0	37.0
7	34.27992668873172	35.0	33.0	37.0	31.0	37.0
8	34.68835287703212	35.0	35.0	37.0	32.0	37.0
9	36.41757217857847	39.0	37.0	39.0	32.0	39.0
10	36.38010766312653	39.0	35.0	39.0	32.0	39.0
11	36.36398123190576	38.0	35.0	39.0	32.0	39.0
12	34.96015747588075	37.0	33.0	39.0	29.0	39.0
13	34.585125798962274	37.0	33.0	39.0	25.0	39.0
14	35.38058889631217	37.0	33.0	40.0	26.0	41.0
15	36.12718634731213	38.0	34.0	40.0	30.0	41.0
16	35.76005918144283	36.0	34.0	40.0	30.0	41.0
17	35.325211243450234	36.0	33.0	40.0	29.0	41.0
18	34.98048701822287	36.0	33.0	39.0	29.0	40.0
19	34.35448455574242	36.0	32.0	38.0	27.0	40.0
20	34.776669405600224	35.0	34.0	38.0	29.0	40.0
21	34.966359752523275	35.0	34.0	39.0	30.0	40.0
22	35.349219147961286	35.0	34.0	39.0	30.0	40.0
23	35.71701440883831	35.0	34.0	39.0	31.0	41.0
24	35.42251761748617	35.0	34.0	39.0	31.0	41.0
25	35.06461835392653	35.0	34.0	39.0	30.0	41.0
26	35.091214166685866	35.0	34.0	39.0	30.0	41.0
27	35.33899038301554	35.0	34.0	40.0	31.0	41.0
28	35.29926304769072	35.0	34.0	40.0	30.0	41.0
29	35.45157079119343	36.0	35.0	40.0	31.0	41.0
30	35.087156961370795	36.0	34.0	40.0	30.0	41.0
31	34.342684103690395	35.0	34.0	39.0	27.0	40.0
32	34.186598167730246	35.0	34.0	39.0	25.0	40.0
33	33.81947612136291	35.0	33.0	39.0	23.0	41.0
34	33.50411479971228	35.0	33.0	40.0	21.0	41.0
35	33.22801749845775	35.0	33.0	40.0	18.0	41.0
36	32.808167141500476	35.0	33.0	39.0	16.0	41.0
37	32.69597581547267	35.0	33.0	39.0	15.0	40.0
38	32.58118890194362	35.0	32.0	39.0	15.0	40.0
39	32.42726339581686	35.0	32.0	39.0	15.0	40.0
40	32.245738654543686	35.0	31.0	39.0	15.0	40.0
41	31.949117269871987	35.0	31.0	38.0	12.0	40.0
42	32.12928278337083	35.0	32.0	39.0	12.0	40.0
43	31.882136265784066	35.0	31.0	38.0	10.0	40.0
44	31.704215653901187	35.0	31.0	38.0	10.0	40.0
45	31.671128312637748	35.0	31.0	38.0	10.0	40.0
46	31.496494421022724	35.0	31.0	38.0	10.0	40.0
47	31.475944740095684	35.0	31.0	38.0	10.0	40.0
48	31.570565935345808	35.0	31.0	38.0	10.0	40.0
49	31.54438992174841	35.0	31.0	38.0	10.0	40.0
50	31.619933292889268	35.0	31.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	5.0
12	5.0
13	3.0
14	26.0
15	64.0
16	115.0
17	213.0
18	562.0
19	932.0
20	1411.0
21	1953.0
22	2816.0
23	3900.0
24	5689.0
25	8663.0
26	11949.0
27	13949.0
28	12957.0
29	12248.0
30	13410.0
31	16327.0
32	22185.0
33	35193.0
34	57945.0
35	37167.0
36	31850.0
37	42740.0
38	46021.0
39	10363.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.806385554813225	21.55796684098571	27.72671074557867	24.908936858622393
2	37.47961798276263	20.589101092245745	29.53415091779872	12.3971300071929
3	27.12235353744788	18.945229008122087	41.25269093822553	12.679726516204504
4	25.18564594036292	23.57428269377955	37.614772834898616	13.625298530958908
5	19.885937496000388	28.79361495713697	37.711531422223246	13.608916124639395
6	17.444702979294174	36.070731039284496	35.56722802005821	10.917337961363119
7	73.11007185221021	5.5820489782753935	18.556914783329876	2.750964386184512
8	74.77621376992445	3.9801568103454894	17.834553054678842	3.4090763650512077
9	71.1518623468309	4.921889198618758	19.46895406014903	4.457294394401313
10	50.03391670058337	17.556564097444603	22.67760192288494	9.73191727908709
11	45.420221520850454	15.7644824311491	27.7190314926164	11.096264555384053
12	39.55583200866219	18.41177690234294	30.471019779195878	11.561371309798982
13	18.64625009279097	38.55624924807315	31.15293744224562	11.64456321689026
14	12.407880961340082	39.25224554155371	34.85075371867825	13.489119778427956
15	11.007953146317927	19.242928047959495	56.706675574600105	13.042443231122478
16	12.20258893214868	14.438787394762237	54.38242167801916	18.976201995069918
17	12.758822821715905	16.011242426336768	36.821250028797195	34.40868472315014
18	18.429439184156166	19.617163642320875	42.82744974568874	19.12594742783422
19	25.225834030865478	20.948234155781325	33.66123743482234	20.164694378530857
20	27.59846722110873	18.921167348840303	33.33563710922201	20.14472832082895
21	17.974827408789672	26.045210321939884	35.85903963262454	20.120922636645908
22	19.593869908335318	21.14866265809662	33.141607984375284	26.11585944919278
23	15.95825558089709	26.574310851040412	33.17590864760676	24.291524920455736
24	16.700839342348775	19.34710991314765	47.599849486641936	16.352201257861633
25	14.711912825120372	21.261035726444533	43.96807478568485	20.058976662750247
26	14.624625316449217	30.482538658639285	35.488899639843034	19.40393638506846
27	15.13606356373652	30.278014554744114	36.191295310792164	18.394626570727198
28	12.832287675054971	24.44280620381249	46.83960344337702	15.885302677755508
29	13.10182945403071	20.555568354310495	45.83157350452948	20.511028687129315
30	16.284623831793642	27.03941760545534	39.01444467482204	17.661513887928983
31	26.134033681203494	23.683072110745066	31.763438052746228	18.419456155305213
32	27.14590324653218	23.196463448035775	32.93657193028262	16.721061375149425
33	24.645794457115212	24.351423093561458	31.815912947988416	19.18686950133491
34	17.032071120121433	24.92480731474442	33.91772448376222	24.125397081371926
35	18.365445409470567	23.116343242129407	36.40298671745212	22.115224630947903
36	27.538825023101754	22.768729058037234	30.902337820576815	18.7901080982842
37	18.515958767531092	29.274336192575184	32.82035923545358	19.389345804440143
38	19.12492352743925	30.41291343178136	28.472622183314005	21.989540857465386
39	19.334055183111786	28.555814090405285	31.182886528798477	20.92724419768445
40	21.91479612863261	24.345791641389127	29.2116222933833	24.527789936594967
41	16.44895984518626	22.336643091360074	31.07998453910404	30.134412524349628
42	20.635944535315602	23.958757292090628	27.220136025167474	28.185162147426297
43	21.18501112211804	24.79323611399083	28.838410599416886	25.183342164474237
44	18.01219977320606	28.84173827570054	30.495081438477666	22.65098051261573
45	15.739652846571087	34.99281989848028	26.966720677412503	22.300806577536136
46	21.802679035383438	30.13518044964586	28.880902465808127	19.181238049162577
47	20.580397938888506	24.849550635714156	30.640731269662087	23.92932015573525
48	21.676227336604693	21.57102157102157	33.45517748033471	23.29757361203902
49	19.604364887383756	21.44584974773654	35.995474360254235	22.95431100462547
50	17.954349400890283	30.068626923972836	30.518887122660708	21.458136552476176
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	196.0
1	349.5
2	503.0
3	5443.5
4	10384.0
5	7464.5
6	4545.0
7	4842.0
8	5139.0
9	5487.5
10	5836.0
11	6044.0
12	6252.0
13	5861.0
14	5470.0
15	5079.0
16	4688.0
17	4230.5
18	3773.0
19	3444.5
20	3116.0
21	2965.0
22	2814.0
23	2595.5
24	2377.0
25	2320.5
26	2264.0
27	2336.0
28	2408.0
29	2622.5
30	2837.0
31	3811.5
32	4786.0
33	5456.0
34	6126.0
35	7163.5
36	8201.0
37	8267.0
38	8333.0
39	12033.0
40	15733.0
41	23530.5
42	31328.0
43	37971.0
44	44614.0
45	43264.5
46	41915.0
47	38249.0
48	34583.0
49	31729.5
50	28876.0
51	26920.5
52	24965.0
53	22087.5
54	19210.0
55	16699.5
56	14189.0
57	12649.5
58	11110.0
59	10282.5
60	9455.0
61	8568.5
62	7682.0
63	6526.5
64	5371.0
65	4655.0
66	3939.0
67	3283.5
68	2628.0
69	2243.5
70	1859.0
71	1641.5
72	1424.0
73	1057.5
74	691.0
75	604.0
76	517.0
77	372.0
78	227.0
79	178.0
80	129.0
81	100.0
82	71.0
83	53.5
84	36.0
85	38.0
86	40.0
87	28.5
88	17.0
89	11.0
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	390663.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.84289121859548
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.00231986957881	23.74974175316913
2	7.999733656612144	4.934698298972508
3	3.5179617587695486	3.2551233551072407
4	2.0398823657587806	2.516634796233171
5	1.3468453575256825	2.077030242521748
6	0.8745951412074867	1.61850256803448
7	0.63451009823081	1.3699088155783243
8	0.49165412660651514	1.2131227795278654
9	0.3870101475635707	1.0742860693616219
>10	4.719692203971806	34.29592207352757
>50	0.9044069533078269	18.49235406735932
>100	0.0788968416567893	3.313531487593985
>500	0.001660986140142933	0.2943141372329537
>1k	0.0	0.0
>5k	8.304930700714665E-4	1.7948295557800753
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7007	1.7936175168879571	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCC	640	0.16382406319513237	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT	509	0.13029132525987872	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	436	0.11160514305168392	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0588742727107507	0.0
2	0.0	0.0	0.0	0.27210152996316517	0.0
3	0.0	0.0	0.0	0.3519657607707922	0.0
4	0.0	0.0	0.0	0.5459948856175272	0.0
5	2.5597509874239434E-4	0.0	0.0	1.012637490624912	0.0
6	2.5597509874239434E-4	0.0	0.0	1.3149440822396796	0.0
7	2.5597509874239434E-4	0.0	0.0	1.5842298861166786	0.0
8	2.5597509874239434E-4	0.0	0.0	2.241573939687147	0.0
9	2.5597509874239434E-4	0.0	0.0	2.493965387047148	0.0
10	2.5597509874239434E-4	0.0	0.0	3.057366579379158	0.0
11	2.5597509874239434E-4	0.0	0.0	3.3496901421429723	0.0
12	2.5597509874239434E-4	0.0	0.0	3.741076068120093	0.0
13	2.5597509874239434E-4	0.0	0.0	3.8782787210460166	0.0
14	2.5597509874239434E-4	0.0	0.0	3.9202586372397694	0.0
15	2.5597509874239434E-4	0.0	0.0	3.9978190921587147	0.0
16	2.5597509874239434E-4	0.0	0.0	4.136557595677092	0.0
17	2.5597509874239434E-4	0.0	0.0	4.307292986538269	0.0
18	2.5597509874239434E-4	0.0	0.0	4.575810865119041	0.0
19	2.5597509874239434E-4	0.0	0.0	4.673849327937378	0.0
20	2.5597509874239434E-4	0.0	0.0	4.778031193125533	0.0
21	2.5597509874239434E-4	0.0	0.0	4.901411190719367	0.0
22	5.119501974847887E-4	0.0	0.0	5.0183918108446415	0.0
23	5.119501974847887E-4	0.0	0.0	5.168137243608942	0.0
24	5.119501974847887E-4	0.0	0.0	5.276414710376975	0.0
25	5.119501974847887E-4	0.0	0.0	5.350903464111012	0.0
26	7.67925296227183E-4	0.0	0.0	5.430511719819896	0.0
27	7.67925296227183E-4	0.0	0.0	5.513703626911174	0.0
28	0.0010239003949695773	0.0	0.0	5.602271011076042	0.0
29	0.0010239003949695773	0.0	0.0	5.684183042673609	0.0
30	0.0010239003949695773	0.0	0.0	5.799883787305171	0.0
31	0.0012798754937119717	0.0	0.0	5.913280756048052	0.0
32	0.0012798754937119717	0.0	0.0	6.015414820446267	0.0
33	0.0012798754937119717	0.0	0.0	6.114733158758316	0.0
34	0.0012798754937119717	0.0	0.0	6.210467845687972	0.0
35	0.0012798754937119717	0.0	0.0	6.357653527464848	0.0
36	0.0012798754937119717	0.0	0.0	6.449036637715883	0.0
37	0.0012798754937119717	0.0	0.0	6.54809900092919	0.0
38	0.0012798754937119717	0.0	0.0	6.643321737661361	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGG	20	7.8500924E-4	44.000004	2
ATTTACG	20	7.8500924E-4	44.000004	1
CGGAAAT	20	7.8500924E-4	44.000004	39
TCGTTGA	80	0.0	44.000004	24
GACCGTC	20	7.8500924E-4	44.000004	10
CGTTGAT	80	0.0	44.000004	25
CGATTCT	20	7.8500924E-4	44.000004	10
CCGTCGG	20	7.8500924E-4	44.000004	12
ATCGTAG	20	7.8500924E-4	44.000004	1
CACTCGC	20	7.8500924E-4	44.000004	10
GCTTAAG	20	7.8500924E-4	44.000004	1
CGGGATA	20	7.8500924E-4	44.000004	6
ACTCGCT	20	7.8500924E-4	44.000004	11
CTACAAC	20	7.8500924E-4	44.000004	40
AACTCGT	20	7.8500924E-4	44.000004	44
GACGAGG	40	8.289135E-9	44.000004	2
AGAATGC	20	7.8500924E-4	44.000004	21
TATCCAG	20	7.8500924E-4	44.000004	1
AGGTATG	30	2.5234112E-6	44.0	1
GTCGATG	35	1.4428042E-7	44.0	1
>>END_MODULE