FastQCFastQC Report
Thu 26 May 2016
SRR1546052_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546052_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences393491
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71751.8234216284489353No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCC5570.14155342815972918No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT5260.13367523018315539No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA4720.11995191757880105No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC1100.044.0000049
TCACGAC351.4428406E-744.025
GTGCATG408.289135E-944.01
AAATCGT207.850162E-444.010
CGTATGA207.850162E-444.015
GTATTAG302.5234567E-644.01
GGCACGA207.850162E-444.033
ATAATGC207.850162E-444.035
GACGTAG207.850162E-444.01
GCAAATG207.850162E-444.01
GAATCAT207.850162E-444.027
CCGTGAC207.850162E-444.031
GTTAGAG454.783942E-1044.01
AGGGTCC351.4428406E-744.06
TACGGGC254.437486E-544.04
CGACACC254.437486E-544.019
ACGGGTA302.5234567E-644.05
TCGATAG302.5234567E-644.01
ATCGTAT207.850162E-444.013
ATCGTAG351.4428406E-744.01
Produced by FastQC (version 0.11.5)