##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546052_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	393491
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.10701896612629	31.0	28.0	33.0	25.0	33.0
2	28.77052588242171	31.0	28.0	33.0	16.0	33.0
3	28.24618352135119	31.0	27.0	33.0	16.0	34.0
4	32.11993921080787	35.0	32.0	35.0	25.0	37.0
5	33.66002526106061	35.0	33.0	35.0	28.0	37.0
6	33.52904640766879	35.0	33.0	37.0	28.0	37.0
7	34.32664279487968	35.0	35.0	37.0	31.0	37.0
8	34.72282720570483	35.0	35.0	37.0	32.0	37.0
9	36.523069142623335	39.0	37.0	39.0	32.0	39.0
10	36.398888411679046	39.0	35.0	39.0	32.0	39.0
11	36.4257657735501	39.0	35.0	39.0	32.0	39.0
12	34.91962204980546	35.0	33.0	39.0	29.0	39.0
13	34.61706366854642	37.0	33.0	39.0	25.0	39.0
14	35.40121375075923	37.0	33.0	40.0	26.0	41.0
15	36.16701779710336	38.0	34.0	40.0	31.0	41.0
16	35.7903738586143	36.0	34.0	40.0	30.0	41.0
17	35.361505091603114	36.0	33.0	40.0	29.0	41.0
18	35.05887555242687	36.0	33.0	39.0	29.0	40.0
19	34.42013921538231	36.0	32.0	38.0	27.0	40.0
20	34.75368178687695	35.0	34.0	39.0	29.0	40.0
21	34.88436076047483	35.0	34.0	39.0	29.0	40.0
22	35.316401137510134	35.0	34.0	39.0	30.0	40.0
23	35.71725147462077	35.0	34.0	39.0	31.0	41.0
24	35.28140923172322	35.0	34.0	39.0	31.0	41.0
25	34.94553115573164	35.0	34.0	39.0	30.0	40.0
26	35.04644070639481	35.0	34.0	39.0	30.0	40.0
27	35.35828265449528	35.0	34.0	39.0	31.0	41.0
28	35.31884083752868	35.0	34.0	40.0	31.0	41.0
29	35.46795733574593	36.0	35.0	40.0	31.0	41.0
30	35.09994383607249	36.0	34.0	40.0	30.0	41.0
31	34.35648591708578	35.0	34.0	39.0	27.0	40.0
32	34.22999763654061	35.0	34.0	39.0	25.0	40.0
33	33.87931871376982	35.0	33.0	39.0	23.0	41.0
34	33.49566826178998	35.0	33.0	40.0	21.0	41.0
35	33.249609266793904	35.0	33.0	40.0	18.0	41.0
36	32.81670482933536	35.0	33.0	39.0	16.0	41.0
37	32.71120051030392	35.0	33.0	39.0	15.0	40.0
38	32.626415851950874	35.0	32.0	39.0	15.0	40.0
39	32.42476956270919	35.0	32.0	39.0	15.0	40.0
40	32.20745074220249	35.0	31.0	39.0	15.0	40.0
41	31.91527125143904	35.0	31.0	38.0	12.0	40.0
42	32.092093592991965	35.0	32.0	39.0	12.0	40.0
43	31.854024615556646	35.0	31.0	38.0	10.0	40.0
44	31.66651079694326	35.0	31.0	38.0	10.0	40.0
45	31.609005034422644	35.0	31.0	38.0	10.0	40.0
46	31.450610052072346	35.0	31.0	38.0	10.0	40.0
47	31.435750754146856	35.0	31.0	38.0	10.0	40.0
48	31.535636139073066	35.0	31.0	38.0	10.0	40.0
49	31.57014264621046	35.0	31.0	38.0	10.0	40.0
50	31.662711472435202	35.0	31.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	2.0
14	21.0
15	46.0
16	111.0
17	239.0
18	480.0
19	941.0
20	1325.0
21	1980.0
22	2782.0
23	3909.0
24	5809.0
25	8679.0
26	12198.0
27	13894.0
28	12921.0
29	12715.0
30	13097.0
31	16385.0
32	22114.0
33	35583.0
34	59153.0
35	37343.0
36	32401.0
37	43979.0
38	45995.0
39	9386.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.64963366379409	21.58168801827742	27.676617762540946	25.092060555387548
2	37.65727805718555	20.570737323089983	29.38669499429466	12.385289625429806
3	27.019423570043532	18.90259243540513	41.33055139761773	12.747432596933603
4	25.0145492527148	23.613500689977663	37.752324703741635	13.619625353565901
5	19.890670942918643	28.706882749541922	37.80543900622886	13.597007301310576
6	17.52009575822573	36.036148221941545	35.67095562541456	10.77280039441817
7	72.86011624154047	5.594282969623193	18.761801413501196	2.783799375335141
8	74.60348521313067	4.01076517633186	18.026079376656647	3.3596702338808258
9	71.05931266534685	4.952336902241729	19.61823777418035	4.370112658231065
10	49.949045848570876	17.30890922536983	22.81119517346013	9.93084975259917
11	45.42492712666872	15.751313244775611	27.724903492074787	11.098856136480885
12	39.61539145749204	18.14577715881685	30.441611116899754	11.797220266791362
13	18.694455527572423	38.48016854260962	31.22307752909215	11.602298400725811
14	12.2971046351759	39.42758538314726	34.89914635912893	13.37616362254791
15	10.905713218345529	19.179091770841012	56.80917733823645	13.106017672577009
16	12.210444457433587	14.443024109827164	54.47570592465901	18.870825508080237
17	12.746670190677806	15.92565014193463	36.96221768731686	34.3654619800707
18	18.345273462417182	19.633231763877703	42.89882106579312	19.122673707911996
19	25.175416972688065	21.020302878591888	33.69861064166652	20.105669507053527
20	27.6006312723798	18.83423000780196	33.42871882711422	20.136419892704026
21	18.095966616771413	25.89029990520749	36.05292115956909	19.96081231845201
22	19.53945579441461	21.071384097730316	33.269121784233946	26.120038323621124
23	15.939373454538986	26.57544899375081	33.35527369113906	24.129903860571144
24	16.558447334246527	19.23906772963041	47.72205717538648	16.480427760736585
25	14.587373027591482	21.360336068677555	44.121974835510855	19.93031606822011
26	14.689535465868342	30.407048699970268	35.4795916552094	19.423824178951996
27	15.13859275053305	30.13791929167376	36.41658894358448	18.30689901420871
28	12.758614555351965	24.45214757135487	46.8719742001723	15.91726367312086
29	12.953028150580318	20.618006510949424	45.99774836019121	20.431216978279046
30	16.19376300855674	26.96275137169592	39.15693116233916	17.686554457408175
31	26.15460074055061	23.76953983699754	31.75371228312719	18.32214713932466
32	27.175208581644817	23.07600427964045	33.04776983463408	16.701017304080654
33	24.55812204091072	24.355067841449994	31.886625107054545	19.20018501058474
34	16.93609256628487	24.905525158135763	33.94054756017291	24.217834715406454
35	18.30689901420871	23.040679456455166	36.60337847625485	22.049043053081267
36	27.597327511938012	22.68158610997456	30.887364641122666	18.83372173696476
37	18.558493078621872	29.111974606788976	33.02972621991354	19.29980609467561
38	19.055836092820417	30.435003596016173	28.474602976942293	22.034557334221113
39	19.254061719327762	28.49391726875583	31.188769247581266	21.063251764335146
40	21.924262562549078	24.34515656012463	29.221761107623806	24.508819769702484
41	16.53913304243299	22.285897263215677	31.014686485840848	30.160283208510485
42	20.506186926765796	24.11846776673418	27.343446228757458	28.031899077742565
43	21.107979598008594	24.818102574137654	28.877915886259153	25.1960019415946
44	18.02684178291244	28.780836156354273	30.595617180570837	22.596704880162445
45	15.604168837406702	34.99343059942921	27.055510799484612	22.346889763679474
46	21.601764716346754	30.409335918737657	28.822768500423134	19.16613086449245
47	20.482552332836075	24.950252991809215	30.749369108823327	23.817825566531383
48	21.648525633368997	21.649796310461994	33.44930379602074	23.25237426014826
49	19.661440795342205	21.414975183676376	36.030557242732364	22.893026778249055
50	18.041327501772596	30.145543354231734	30.46677052334107	21.346358620654602
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	189.0
1	342.0
2	495.0
3	5648.0
4	10801.0
5	7778.5
6	4756.0
7	5003.0
8	5250.0
9	5733.0
10	6216.0
11	6196.0
12	6176.0
13	5840.0
14	5504.0
15	5085.0
16	4666.0
17	4245.0
18	3824.0
19	3400.5
20	2977.0
21	2931.5
22	2886.0
23	2602.0
24	2318.0
25	2297.0
26	2276.0
27	2396.0
28	2516.0
29	2662.5
30	2809.0
31	3768.5
32	4728.0
33	5438.0
34	6148.0
35	7131.5
36	8115.0
37	8351.5
38	8588.0
39	12256.0
40	15924.0
41	23651.5
42	31379.0
43	38412.5
44	45446.0
45	43693.5
46	41941.0
47	38134.5
48	34328.0
49	31879.0
50	29430.0
51	27040.5
52	24651.0
53	21851.5
54	19052.0
55	16579.0
56	14106.0
57	12510.0
58	10914.0
59	10320.5
60	9727.0
61	8793.5
62	7860.0
63	6731.0
64	5602.0
65	4822.0
66	4042.0
67	3345.0
68	2648.0
69	2312.0
70	1976.0
71	1744.5
72	1513.0
73	1100.5
74	688.0
75	598.0
76	508.0
77	362.0
78	216.0
79	173.0
80	130.0
81	111.5
82	93.0
83	57.5
84	22.0
85	28.0
86	34.0
87	25.0
88	16.0
89	10.0
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	393491.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.732151902094486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.78740679028905	23.598422496470846
2	7.991138263996741	4.911697499995748
3	3.5475798330791357	3.2707428694498497
4	2.1197438301725273	2.6057715752955866
5	1.3244693557681129	2.0351896715567435
6	0.9461590925606463	1.7446502973672995
7	0.6602675906122168	1.4204010723507206
8	0.48877134527059946	1.2016796182597704
9	0.38521656052783176	1.065468046800933
>10	4.764253827345525	34.40095939152194
>50	0.9054650183706594	18.3413055841869
>100	0.07704322663172125	3.301292433721323
>500	0.0016568435834778763	0.2757229664317261
>1k	0.0	0.0
>5k	8.284217917389382E-4	1.8266964765906142
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7175	1.8234216284489353	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCC	557	0.14155342815972918	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT	526	0.13367523018315539	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	472	0.11995191757880105	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06683761509157769	0.0
2	0.0	0.0	0.0	0.2518481998317624	0.0
3	0.0	0.0	0.0	0.326818148318513	0.0
4	0.0	0.0	0.0	0.5047129413379213	0.0
5	0.0	0.0	0.0	0.9583446635374125	0.0
6	0.0	0.0	0.0	1.2503462595078414	0.0
7	0.0	0.0	0.0	1.5070230322929876	0.0
8	0.0	0.0	0.0	2.145157068395465	0.0
9	0.0	0.0	0.0	2.397767674483025	0.0
10	0.0	0.0	0.0	2.938313709843427	0.0
11	0.0	0.0	0.0	3.222182972418683	0.0
12	0.0	0.0	0.0	3.6079605378522	0.0
13	0.0	0.0	0.0	3.747480882663136	0.0
14	0.0	0.0	0.0	3.7876342788018023	0.0
15	0.0	0.0	0.0	3.864637310637346	0.0
16	0.0	0.0	0.0	4.012289988843455	0.0
17	0.0	0.0	0.0	4.170870490049328	0.0
18	0.0	0.0	0.0	4.4468615546480095	0.0
19	0.0	0.0	0.0	4.551311211692262	0.0
20	0.0	0.0	0.0	4.658048087503907	0.0
21	0.0	0.0	0.0	4.7868947447336785	0.0
22	2.5413541859915475E-4	0.0	0.0	4.901001547684699	0.0
23	2.5413541859915475E-4	0.0	0.0	5.03010234033307	0.0
24	2.5413541859915475E-4	0.0	0.0	5.1376016224005125	0.0
25	2.5413541859915475E-4	0.0	0.0	5.217908414677845	0.0
26	2.5413541859915475E-4	0.0	0.0	5.310667842466537	0.0
27	2.5413541859915475E-4	0.0	0.0	5.385891926371887	0.0
28	2.5413541859915475E-4	0.0	0.0	5.469502479091008	0.0
29	2.5413541859915475E-4	0.0	0.0	5.550571677624139	0.0
30	5.082708371983095E-4	0.0	0.0	5.674081491063328	0.0
31	5.082708371983095E-4	0.0	0.0	5.785646939828357	0.0
32	5.082708371983095E-4	0.0	0.0	5.876373284268255	0.0
33	7.624062557974642E-4	0.0	0.0	5.957950753638584	0.0
34	7.624062557974642E-4	0.0	0.0	6.064941764868828	0.0
35	7.624062557974642E-4	0.0	0.0	6.207257599284355	0.0
36	7.624062557974642E-4	0.0	0.0	6.306370412538025	0.0
37	7.624062557974642E-4	0.0	0.0	6.404212548698699	0.0
38	7.624062557974642E-4	0.0	0.0	6.495955434812995	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAC	110	0.0	44.000004	9
TCACGAC	35	1.4428406E-7	44.0	25
GTGCATG	40	8.289135E-9	44.0	1
AAATCGT	20	7.850162E-4	44.0	10
CGTATGA	20	7.850162E-4	44.0	15
GTATTAG	30	2.5234567E-6	44.0	1
GGCACGA	20	7.850162E-4	44.0	33
ATAATGC	20	7.850162E-4	44.0	35
GACGTAG	20	7.850162E-4	44.0	1
GCAAATG	20	7.850162E-4	44.0	1
GAATCAT	20	7.850162E-4	44.0	27
CCGTGAC	20	7.850162E-4	44.0	31
GTTAGAG	45	4.783942E-10	44.0	1
AGGGTCC	35	1.4428406E-7	44.0	6
TACGGGC	25	4.437486E-5	44.0	4
CGACACC	25	4.437486E-5	44.0	19
ACGGGTA	30	2.5234567E-6	44.0	5
TCGATAG	30	2.5234567E-6	44.0	1
ATCGTAT	20	7.850162E-4	44.0	13
ATCGTAG	35	1.4428406E-7	44.0	1
>>END_MODULE