FastQCFastQC Report
Thu 26 May 2016
SRR1546051_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546051_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences387881
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71751.8497941378928073No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCC5660.14592104279405282No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT5660.14592104279405282No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA4530.11678839644117656No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATAGA207.850023E-444.00000440
TACCAGG207.850023E-444.0000042
ACGCAGC207.850023E-444.00000437
CCGTGTT207.850023E-444.00000435
GATCATA207.850023E-444.0000049
CGACACT207.850023E-444.00000417
ACTTAGT207.850023E-444.00000412
CCTCATG408.287316E-944.00000438
CTCATGT207.850023E-444.00000439
CGGGATA207.850023E-444.0000046
ACGCTTC207.850023E-444.00000419
AGCGAGT207.850023E-444.00000429
GCGATAT207.850023E-444.0000049
TCCAAGG207.850023E-444.0000042
TACTACG207.850023E-444.00000444
GACGATT207.850023E-444.00000413
CTTAGTA207.850023E-444.00000413
TTACACG408.287316E-944.00000434
GCGTAAG207.850023E-444.0000041
TGCGTAG207.850023E-444.0000041
Produced by FastQC (version 0.11.5)