FastQCFastQC Report
Thu 26 May 2016
SRR1546050_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546050_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences335058
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37671.1242829599651405No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTT6950.20742677387198633No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATT207.8484876E-444.00000438
GATACAA207.8484876E-444.0000049
GTCGATG207.8484876E-444.0000041
CCCTATG207.8484876E-444.00000433
CGTGCGG408.281859E-944.0000042
CGTGCCC207.8484876E-444.00000424
TAGACAG207.8484876E-444.0000041
GCGAAAG207.8484876E-444.0000041
GGGTATA408.281859E-944.0000047
CATGAAC207.8484876E-444.00000432
TAGCGAG207.8484876E-444.0000041
GTAGGTT207.8484876E-444.00000417
CTAAGCG207.8484876E-444.0000041
TCGATAC207.8484876E-444.00000425
TCTAGAG207.8484876E-444.0000041
CGTTATG207.8484876E-444.00000441
AATATAG207.8484876E-444.0000041
GTTAACG207.8484876E-444.0000041
TACGATG207.8484876E-444.0000041
ACGTGCC207.8484876E-444.00000423
Produced by FastQC (version 0.11.5)