##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546050_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	335058
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.01662697204663	28.0	25.0	30.0	16.0	33.0
2	26.08625073867808	30.0	25.0	31.0	10.0	33.0
3	26.34912462916868	30.0	25.0	31.0	16.0	33.0
4	27.873529359096036	32.0	19.0	35.0	10.0	35.0
5	31.17873323424601	33.0	30.0	35.0	19.0	37.0
6	29.777560899903897	33.0	28.0	35.0	17.0	36.0
7	29.723719475434105	32.0	28.0	35.0	17.0	35.0
8	32.3990891129297	33.0	32.0	35.0	28.0	35.0
9	26.947050958341542	30.0	23.0	34.0	10.0	35.0
10	31.752398092270592	34.0	30.0	37.0	17.0	38.0
11	33.659954395955324	35.0	32.0	37.0	26.0	39.0
12	32.94209957678969	35.0	31.0	37.0	23.0	39.0
13	32.349775262790324	35.0	30.0	37.0	21.0	39.0
14	32.14491819326803	35.0	29.0	38.0	20.0	40.0
15	31.478063499453825	33.0	27.0	38.0	17.0	40.0
16	30.22884097678611	33.0	26.0	37.0	17.0	40.0
17	30.08500617803485	32.0	25.0	37.0	17.0	40.0
18	31.072474019423503	33.0	27.0	37.0	17.0	39.0
19	30.581538121758022	34.0	27.0	37.0	18.0	39.0
20	31.270120397065583	34.0	29.0	36.0	18.0	39.0
21	32.12105068376221	34.0	30.0	36.0	23.0	39.0
22	32.52071880092402	34.0	30.0	37.0	23.0	39.0
23	33.899041360003345	35.0	33.0	38.0	28.0	39.0
24	33.59709960663527	35.0	33.0	38.0	26.0	40.0
25	32.381820460935124	34.0	31.0	37.0	23.0	39.0
26	26.0807501984731	33.0	19.0	35.0	0.0	38.0
27	29.19393955673346	33.0	23.0	36.0	11.0	39.0
28	30.73195088611524	33.0	27.0	36.0	18.0	39.0
29	32.045875639441526	34.0	30.0	36.0	23.0	39.0
30	31.718368163123998	34.0	30.0	37.0	21.0	39.0
31	30.57710605328033	33.0	28.0	35.0	18.0	38.0
32	30.737290260193756	34.0	29.0	36.0	18.0	38.0
33	30.438688227112916	33.0	29.0	36.0	16.0	38.0
34	30.47407314554495	34.0	29.0	36.0	15.0	39.0
35	29.698168675274133	33.0	27.0	36.0	10.0	39.0
36	29.328883357508253	33.0	25.0	36.0	10.0	39.0
37	29.3235827826824	33.0	25.0	36.0	10.0	39.0
38	29.14451527795188	33.0	25.0	36.0	10.0	38.0
39	29.04436246858753	33.0	24.0	36.0	10.0	38.0
40	29.033379295524952	33.0	25.0	35.0	10.0	38.0
41	28.415653409260486	32.0	23.0	35.0	9.0	38.0
42	28.62276978911114	33.0	23.0	35.0	8.0	38.0
43	28.157157865205427	32.0	23.0	35.0	8.0	38.0
44	27.9003724728256	32.0	23.0	35.0	8.0	38.0
45	27.384751296790405	31.0	23.0	34.0	8.0	37.0
46	26.96040685493258	31.0	23.0	34.0	7.0	37.0
47	27.177237970739395	31.0	23.0	34.0	8.0	37.0
48	27.409782783876224	31.0	23.0	34.0	8.0	37.0
49	27.223531448286565	31.0	23.0	34.0	8.0	37.0
50	28.841872153477905	33.0	24.0	35.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	4.0
12	16.0
13	65.0
14	125.0
15	317.0
16	606.0
17	1135.0
18	1876.0
19	2816.0
20	4184.0
21	5756.0
22	7854.0
23	9936.0
24	12764.0
25	15896.0
26	18344.0
27	19796.0
28	22022.0
29	25268.0
30	29831.0
31	33839.0
32	34881.0
33	32441.0
34	25382.0
35	18656.0
36	9189.0
37	2005.0
38	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.04867217019143	21.97709053357926	27.495538085943327	26.47869921028598
2	37.77913077735795	17.79602337505745	31.19698679034675	13.22785905723785
3	28.473577708933977	14.771770857582867	43.00837466946022	13.746276764022946
4	26.353347778593555	23.12883142620084	36.98374609769055	13.534074697515058
5	19.12295781625868	28.746664756549613	39.015334658477045	13.115042768714671
6	17.077640289143968	34.60535190922169	37.2998107790293	11.01719702260504
7	75.69256666010064	3.850975055065093	18.796745638068632	1.6597126467656345
8	78.25958490768762	2.3237767789457346	17.49995523163154	1.9166830817351026
9	66.05990604611739	2.770266640402557	17.712754209718913	13.457073103761138
10	52.28318679154057	16.501322159148565	22.791576383790268	8.423914665520597
11	47.887828375982664	14.621946051131445	27.655808845035786	9.834416727850105
12	41.06721821296611	17.979275229960187	30.768702732064302	10.184803825009402
13	18.163720908021894	39.871305863462446	31.234890675644216	10.730082552871444
14	11.3986235218977	39.798781106554685	35.94362767043318	12.858967701114436
15	9.325848062126557	17.21433304084666	57.84819344710468	15.611625449922103
16	10.351043699896733	12.036423544580341	55.52113365447177	22.091399101051163
17	11.739161577995452	14.505846748921083	38.05132245760435	35.70366921547911
18	17.926448555175522	18.819129822299423	44.52482853714879	18.729593085376266
19	25.988933259316298	19.98489813703896	34.561777363919084	19.46439123972566
20	28.381951781482613	18.03807102053973	34.195870565693106	19.38410663228456
21	17.53487455903157	25.496182750449176	36.92196574921357	20.046976941305683
22	19.02983960985859	20.417659032167563	33.688197267338786	26.864304090635056
23	14.999791080947178	26.367076744921775	34.30480692894962	24.328325245181432
24	15.52716246142459	18.420094431411872	50.6279509816211	15.424792125542444
25	13.58361835861254	19.969378435972278	46.56417694846862	19.882826256946558
26	11.406084916641298	42.05182386333112	29.890347342848106	16.651743877179474
27	13.795820425120429	31.37247879471614	37.26698064215748	17.564720138005956
28	11.702451515856955	24.35458935467889	48.827068746306615	15.115890383157543
29	11.846008750723755	20.21261990461353	47.805454578013354	20.135916766649355
30	15.589241265691314	27.026664040255717	40.174536945842206	17.209557748210756
31	27.27796381522005	23.221352721021436	32.23979131971181	17.260892144046704
32	28.142590238108028	22.019769711512634	33.55210142721559	16.28553862316375
33	25.3296444197721	23.72514609410908	32.016247933193654	18.928961552925166
34	16.77590148571292	24.192826316637717	33.883088898041535	25.14818329960783
35	18.172376125924465	22.541470431984912	36.82735526386477	22.45879817822586
36	28.51953990055453	22.013800595717754	30.523670528684587	18.942988975043125
37	17.899587534098576	30.347880068525452	32.31261453240931	19.439917864966663
38	18.51530182834017	31.246231995654483	28.197207647631156	22.04125852837419
39	19.079681726745818	28.872613099821525	30.886592769013127	21.161112404419534
40	22.106918802117843	23.497722782324253	29.236430707519297	25.158927708038608
41	15.953954240758318	21.36794226671203	30.925690477469576	31.75241301506008
42	19.93714521067994	23.014522858728938	26.32678521330635	30.72154671728477
43	20.682389317670374	23.732309033062933	28.84903509243176	26.736266556834938
44	16.951393490082314	28.533865778462236	30.86749159846952	23.64724913298593
45	14.692083161721254	36.48114654776188	26.2100889995165	22.616681291000365
46	20.813709865157673	30.186117030484272	28.537447247939163	20.46272585641889
47	19.860740528505513	24.41845889368408	30.165523580991948	25.55527699681846
48	21.053668320111743	20.89817285365519	34.25496481206239	23.79319401417068
49	19.282034752192157	21.23900936554268	35.673226724925236	23.805729157339922
50	17.37967754836476	31.27070537041348	30.09001426618675	21.25960281503501
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	121.0
1	214.0
2	307.0
3	3338.0
4	6369.0
5	5052.5
6	3736.0
7	3937.5
8	4139.0
9	4543.5
10	4948.0
11	5238.5
12	5529.0
13	5539.0
14	5549.0
15	5392.5
16	5236.0
17	4890.5
18	4545.0
19	4231.0
20	3917.0
21	3663.5
22	3410.0
23	3102.0
24	2794.0
25	2667.5
26	2541.0
27	2535.5
28	2530.0
29	2597.5
30	2665.0
31	3480.5
32	4296.0
33	4882.5
34	5469.0
35	5992.5
36	6516.0
37	6667.0
38	6818.0
39	9478.0
40	12138.0
41	18511.0
42	24884.0
43	30377.5
44	35871.0
45	35412.5
46	34954.0
47	32018.5
48	29083.0
49	26985.0
50	24887.0
51	23257.0
52	21627.0
53	19149.5
54	16672.0
55	14505.5
56	12339.0
57	11099.5
58	9860.0
59	9162.5
60	8465.0
61	7691.5
62	6918.0
63	5998.5
64	5079.0
65	4282.5
66	3486.0
67	2996.0
68	2506.0
69	2129.5
70	1753.0
71	1543.5
72	1334.0
73	1023.5
74	713.0
75	593.0
76	473.0
77	361.0
78	249.0
79	202.0
80	155.0
81	116.0
82	77.0
83	63.0
84	49.0
85	44.0
86	39.0
87	22.5
88	6.0
89	4.0
90	2.0
91	1.0
92	0.0
93	2.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	335058.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.81066879407623
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.68175384918865	39.6400953233586
2	5.884880709822957	5.509504036003412
3	2.308456199027705	3.2418113567495404
4	1.2738680093415553	2.38522453890627
5	0.7817567011538518	1.8297277007631292
6	0.5734849098509669	1.6107127304060567
7	0.4593301078918816	1.5051084683371918
8	0.3192411228019046	1.1955112371943177
9	0.30894760056665416	1.301583942436518
>10	3.1355105061326696	31.592048667443578
>50	0.2482209586413012	7.315746126303766
>100	0.02318547415880286	1.448593067508792
>500	6.819257105530253E-4	0.22185372012309615
>1k	6.819257105530253E-4	1.20247908446576
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3767	1.1242829599651405	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTT	695	0.20742677387198633	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.045962191620555246	0.0
2	0.0	0.0	0.0	0.18623641280017192	0.0
3	0.0	0.0	0.0	0.24712139390792043	0.0
4	0.0	0.0	0.0	0.3548639340054558	0.0
5	0.0	0.0	0.0	0.6458583290057244	0.0
6	0.0	0.0	0.0	0.8234395239033242	0.0
7	0.0	0.0	0.0	0.9562523503393442	0.0
8	0.0	0.0	0.0	1.3388726727909794	0.0
9	0.0	0.0	0.0	1.5537608414065625	0.0
10	0.0	0.0	0.0	1.9086247754120182	0.0
11	0.0	0.0	0.0	2.110380889278871	0.0
12	0.0	0.0	0.0	2.3685451474073145	0.0
13	0.0	0.0	0.0	2.460767986438169	0.0
14	0.0	0.0	0.0	2.4840475380381903	0.0
15	0.0	0.0	0.0	2.5264282601818193	0.0
16	0.0	0.0	0.0	2.615964997104979	0.0
17	0.0	0.0	0.0	2.7317658435255985	0.0
18	0.0	0.0	0.0	2.899497997361651	0.0
19	0.0	0.0	0.0	2.9687397405822273	0.0
20	0.0	0.0	0.0	3.0335046469566462	0.0
21	0.0	0.0	0.0	3.1084170501823567	0.0
22	0.0	0.0	0.0	3.17586805866447	0.0
23	0.0	0.0	0.0	3.249288182941461	0.0
24	0.0	0.0	0.0	3.3071886061517706	0.0
25	0.0	0.0	0.0	3.3513600630338627	0.0
26	0.0	0.0	0.0	3.4053805609775023	0.0
27	0.0	0.0	0.0	3.465967086295507	0.0
28	0.0	0.0	0.0	3.528344346351975	0.0
29	0.0	0.0	0.0	3.5963922664135763	0.0
30	0.0	0.0	0.0	3.6835413570187847	0.0
31	0.0	0.0	0.0	3.7635275086701405	0.0
32	0.0	0.0	0.0	3.836649177157388	0.0
33	0.0	0.0	0.0	3.9052940087984767	0.0
34	0.0	0.0	0.0	3.9873693509780397	0.0
35	0.0	0.0	0.0	4.084069026855052	0.0
36	0.0	0.0	0.0	4.157190695342299	0.0
37	0.0	0.0	0.0	4.2341922890962165	0.0
38	0.0	0.0	0.0	4.30015101862961	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAATT	20	7.8484876E-4	44.000004	38
GATACAA	20	7.8484876E-4	44.000004	9
GTCGATG	20	7.8484876E-4	44.000004	1
CCCTATG	20	7.8484876E-4	44.000004	33
CGTGCGG	40	8.281859E-9	44.000004	2
CGTGCCC	20	7.8484876E-4	44.000004	24
TAGACAG	20	7.8484876E-4	44.000004	1
GCGAAAG	20	7.8484876E-4	44.000004	1
GGGTATA	40	8.281859E-9	44.000004	7
CATGAAC	20	7.8484876E-4	44.000004	32
TAGCGAG	20	7.8484876E-4	44.000004	1
GTAGGTT	20	7.8484876E-4	44.000004	17
CTAAGCG	20	7.8484876E-4	44.000004	1
TCGATAC	20	7.8484876E-4	44.000004	25
TCTAGAG	20	7.8484876E-4	44.000004	1
CGTTATG	20	7.8484876E-4	44.000004	41
AATATAG	20	7.8484876E-4	44.000004	1
GTTAACG	20	7.8484876E-4	44.000004	1
TACGATG	20	7.8484876E-4	44.000004	1
ACGTGCC	20	7.8484876E-4	44.000004	23
>>END_MODULE